Impact of volunteer-related and methodology-related factors on the reproducibility of brachial artery flow-mediated vasodilation: analysis of 672 individual repeated measurements

Anke van Mil, Arno Greyling, Peter L. Zock, Johanna M. Geleijnse, Maria T. Hopman, Ronald P. Mensink, Koen D. Reesink, Daniel J. Green, Lorenzo Ghiadoni, Dick H. Thijssen*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

20 Citations (Web of Science)


Objectives: Brachial artery flow-mediated dilation (FMD) is a popular technique to examine endothelial function in humans. Identifying volunteer and methodological factors related to variation in FMD is important to improve measurement accuracy and applicability. Methods: Volunteer-related and methodology-related parameters were collected in 672 volunteers from eight affiliated centres worldwide who underwent repeated measures of FMD. All centres adopted contemporary expert-consensus guidelines for FMD assessment. After calculating the coefficient of variation (%) of the FMD for each individual, we constructed quartiles (n =168 per quartile). Based on two regression models (volunteer-related factors and methodology-related factors), statistically significant components of these two models were added to a final regression model (calculated as(3-coefficient and R-2). This allowed us to identify factors that independently contributed to the variation in FMD%. Results: Median coefficient of variation was 17.5%, with healthy volunteers demonstrating a coefficient of variation 9.3%. Regression models revealed age ((3=0.248, P<0.001), hypertension ((3=0,104, P
Original languageEnglish
Pages (from-to)1738-1745
JournalJournal of Hypertension
Issue number9
Publication statusPublished - Sep 2016


  • Doppler
  • endothelial function
  • flow-mediated dilation
  • reproducibility
  • ultrasonography

Cite this