@article{330ab359599c4f638e5bc27b34423188,
title = "Impact of Pre-Blood Collection Factors on Plasma Metabolomic Profiles",
abstract = "Demographic, lifestyle and biospecimen-related factors at the time of blood collection can influence metabolite levels in epidemiological studies. Identifying the major influences on metabolite concentrations is critical to designing appropriate sample collection protocols and considering covariate adjustment in metabolomics analyses. We examined the association of age, sex, and other short-term pre-blood collection factors (time of day, season, fasting duration, physical activity, NSAID use, smoking and alcohol consumption in the days prior to collection) with 133 targeted plasma metabolites (acylcarnitines, amino acids, biogenic amines, sphingolipids, glycerophospholipids, and hexoses) among 108 individuals that reported exposures within 48 h before collection. The differences in mean metabolite concentrations were assessed between groups based on pre-collection factors using two-sided t-tests and ANOVA with FDR correction. Percent differences in metabolite concentrations were negligible across season, time of day of collection, fasting status or lifestyle behaviors at the time of collection, including physical activity or the use of tobacco, alcohol or NSAIDs. The metabolites differed in concentration between the age and sex categories for 21.8% and 14.3% metabolites, respectively. In conclusion, extrinsic factors in the short period prior to collection were not meaningfully associated with concentrations of selected endogenous metabolites in a cross-sectional sample, though metabolite concentrations differed by age and sex. Larger studies with more coverage of the human metabolome are warranted.",
keywords = "metabolites, metabolomics, blood collection, sample handling, processing, age, sex, smoking, alcohol, NSAID, physical activity, confounding, EXERCISE, SAMPLE, IDENTIFICATION, IMPROVEMENTS, VARIABILITY, BIOMARKERS, SERUM, MEN",
author = "Sheetal Hardikar and Albrechtsen, {Richard D.} and David Achaintre and Tengda Lin and Svenja Pauleck and Mary Playdon and Holowatyj, {Andreana N.} and Biljana Gigic and Petra Schrotz-King and Juergen Boehm and Nina Habermann and Stefanie Brezina and Andrea Gsur and {van Roekel}, {Eline H.} and Weijenberg, {Matty P.} and Pekka Keski-Rahkonen and Augustin Scalbert and Jennifer Ose and Ulrich, {Cornelia M.}",
note = "Funding Information: Funding: This research was supported by the National Cancer Institute projects R01 CA189184, R01 CA207371, and U01 CA206110 to C. M. Ulrich., ERANET TRANSCAN Institut National du Cancer 2014-007, and Bundesministerium f{\"u}r Bildung und Forschung grant 01KT1512. The PR{\"A}VENT Study was funded by the National Center for Tumor Diseases (NCT) and German Cancer Research Center (DKFZ). This work was also supported by the National Cancer Institute of the National Institutes of Health under award number P30 CA042014, the European Research Area Network, joint transnational call (JTC) 2012 call on Translational Cancer Research, the German Consortium of Translational Cancer Research and the German Cancer Research Center, Matthias Lackas Foundation, Stiftung LebensBlicke, and Claussen-Simon Stiftung (Germany), and the Huntsman Cancer Foundation. Additionally, S. Hardikar is supported by the National Cancer Institute K07 CA222060. C.M. Ulrich is supported by National Cancer Institute P30 CA042014. M. C. Playdon is funded by the National Cancer Institute R00CA218694, and the Huntsman Cancer Institute Cancer Center Support Grant P30 CA040214. A. N. Holowatyj was supported by the Ruth L. Kirschstein National Research Service Award T32 HG008962 from the National Human Genome Research Institute, E.H. van Roekel is funded by the Wereld Kanker Onderzoek Fonds (WKOF), as part of the World Cancer Research Fund International grant programme (grant number 2016/1620). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Where authors are identified as personnel of the International Agency for Research on Cancer/World Health Organization, the authors alone are responsible for the views expressed in this article, and they do not necessarily represent the decisions, policy, or views of the International Agency for Research on Cancer/World Health Organization. Funding Information: This research was supported by the National Cancer Institute projects R01 CA189184, R01 CA207371, and U01 CA206110 to C. M. Ulrich., ERANET TRANSCAN Institut National du Cancer 2014-007, and Bundesministerium f?r Bildung und Forschung grant 01KT1512. The PR?VENT Study was funded by the National Center for Tumor Diseases (NCT) and German Cancer Research Center (DKFZ). This work was also supported by the National Cancer Institute of the National Institutes of Health under award number P30 CA042014, the European Research Area Network, joint transnational call (JTC) 2012 call on Translational Cancer Research, the German Consortium of Translational Cancer Research and the German Cancer Research Center, Matthias Lackas Foundation, Stiftung LebensBlicke, and Claussen-Simon Stiftung (Germany), and the Huntsman Cancer Foundation. Additionally, S. Hardikar is supported by the National Cancer Institute K07 CA222060. C.M. Ulrich is supported by National Cancer Institute P30 CA042014. M. C. Playdon is funded by the National Cancer Institute R00CA218694, and the Huntsman Cancer Institute Cancer Center Support Grant P30 CA040214. A. N. Holowatyj was supported by the Ruth L. Kirschstein National Research Service Award T32 HG008962 from the National Human Genome Research Institute, E.H. van Roekel is funded by the Wereld Kanker Onderzoek Fonds (WKOF), as part of the World Cancer Research Fund International grant programmeThe authors thank all PR?VENT study participants and the entire PR?VENT study, data management and laboratory team, who recruited the participants, collected, documented and processed the data and the bio samples for scientific assessment. The authors also thank Huntsman Cancer Institute Shared Resources: Cancer Biostatistics, Biospecimen and Molecular Pathology, and Research Informatics for their support of this study. (grant number 2016/1620). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Where authors are identified as personnel of the International Agency for Research on Cancer/World Health Organization, the authors alone are responsible for the views expressed in this article, and they do not necessarily represent the decisions, policy, or views of the International Agency for Research on Cancer/World Health Organization. Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2020",
month = may,
doi = "10.3390/metabo10050213",
language = "English",
volume = "10",
journal = "Metabolites",
issn = "2218-1989",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "5",
}