Image-guided, targeted and triggered drug delivery to tumors using polymer-based microbubbles

Stanley Fokong, Benjamin Theek, Zhuojun Wu, Patrick Koczera, Lia Appold, Samuel Jorge, Ute Resch-Genger, Marc van Zandvoort, Gert Storm, Fabian Kiessling*, Twan Lammers

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

96 Citations (Web of Science)

Abstract

Microbubbles (MB) are routinely used contrast agents for functional and molecular ultrasound (US) imaging. In addition, they have been attracting more and more attention for drug delivery purposes, enabling e. g. US-mediated drug delivery across biological barriers and US-induced triggered drug release from the MB shell. The vast majority of efforts in this regard have thus far focused on phospholipid-based soft-shell MB, which are suboptimal for stably incorporating large amounts of drug molecules because of their relatively thin shell. Using poly(butyl cyanoacrylate) (PBCA)-based hard-shell MB, we show here that both hydrophilic (Rhodamine-B) and hydrophobic (Coumarin-6) model drugs can be efficiently and stably entrapped within the similar to 50 nm shell of PBCA MB. In addition, we demonstrate that model drug loading does not negatively affect the acoustic properties of the MB, and that functionalizing the surface of fluorophore-loaded MB with anti-VEGFR2 antibodies enables image-guided and targeted model drug delivery to tumor blood vessels. Finally, we show both in vitro and in vivo that disintegrating VEGFR2-targeted MB with high-mechanical index US pulses leads to high levels of model drug release. Consequently, these findings indicate that polymer-based MB are highly suitable systems for image-guided, targeted and triggered drug delivery to tumors and tumor blood vessels.
Original languageEnglish
Pages (from-to)75-81
JournalJournal of Controlled Release
Volume163
Issue number1
DOIs
Publication statusPublished - 10 Oct 2012

Keywords

  • Ultrasound
  • Microbubbles
  • Fluorescent reporter
  • Drug delivery
  • Tumor targeting
  • Theranostics

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