Hypoxia-mimetic effects in the secretome of human preadipocytes and adipocytes

A.R. Anja Rosenow, J.P. Noben, F.G. Bouwman, E.C. Mariman, J. Renes

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

White adipose tissue (WAT) regulates energy metabolism by secretion of proteins with endocrine and paracrine effects. Dysregulation of the secretome of obesity-associated enlarged WAT may lead to obesity-related disorders. This can be caused by hypoxia as a result of poorly vascularized WAT. The effect of hypoxia on the secretome of human (pre)adipocytes is largely unknown. Therefore, we investigated the effect of CoCl2, a hypoxia mimetic, on the secretome of human SGBS (pre)adipocytes by a proteomics approach combined with bioinformatic analysis. In addition, regulation of protein secretion was examined by protein turnover experiments. As such, secretome changes were particularly associated with protein down-regulation and extracellular matrix protein dysregulation. The observed up-regulation of collagens in adipocytes may be essential for cell survival while down-regulation of collagens in preadipocytes may indicate a disturbed differentiation process. These CoCl2-induced changes reflect WAT dysfunction that ultimately may lead to obesity-associated complications. In addition, 9 novel adipocyte secreted proteins were identified from which 6 were regulated by CoCl2. Mass spectrometry data have been deposited to the ProteomeXchange with identifier PXD000162.
Original languageEnglish
Pages (from-to)2761-2771
Number of pages11
JournalBiochimica et Biophysica Acta-Proteins and Proteomics
Volume1834
Issue number12
DOIs
Publication statusPublished - Dec 2013

Keywords

  • Human (pre)adipocyte
  • Hypoxia-mimetic
  • Secretome
  • Turnover
  • INDUCIBLE FACTOR 1-ALPHA
  • WHITE ADIPOSE-TISSUE
  • EXTRACELLULAR-MATRIX
  • ADIPOKINE EXPRESSION
  • PROTEOMICS DATA
  • DIFFERENTIATION
  • PROTEIN
  • OBESITY
  • CELLS
  • METABOLISM

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