TY - JOUR
T1 - Hypertension Is a Conditional Factor for the Development of Cardiac Hypertrophy in Type 2 Diabetic Mice
AU - van Bilsen, Marc
AU - Daniels, Anneleen
AU - Brouwers, Olaf
AU - Janssen, Ben J. A.
AU - Derks, Wouter J. A.
AU - Brouns, Agnieszka E.
AU - Munts, Chantal
AU - Schalkwijk, Casper G.
AU - van der Vusse, Ger J.
AU - van Nieuwenhoven, Frans A.
PY - 2014/1/9
Y1 - 2014/1/9
N2 - Background: Type 2 diabetes is frequently associated with co-morbidities, including hypertension. Here we investigated if hypertension is a critical factor in myocardial remodeling and the development of cardiac dysfunction in type 2 diabetic db/db mice. Methods: Thereto, 14-wks-old male db/db mice and non-diabetic db/+ mice received vehicle or angiotensin II (AngII) for 4 wks to induce mild hypertension (n = 9-10 per group). Left ventricular (LV) function was assessed by serial echocardiography and during a dobutamine stress test. LV tissue was subjected to molecular and (immuno) histochemical analysis to assess effects on hypertrophy, fibrosis and inflammation. Results: Vehicle-treated diabetic mice neither displayed marked myocardial structural remodeling nor cardiac dysfunction. AngII-treatment did not affect body weight and fasting glucose levels, and induced a comparable increase in blood pressure in diabetic and control mice. Nonetheless, AngII-induced LV hypertrophy was significantly more pronounced in diabetic than in control mice as assessed by LV mass (increase +51% and +34%, respectively, p
AB - Background: Type 2 diabetes is frequently associated with co-morbidities, including hypertension. Here we investigated if hypertension is a critical factor in myocardial remodeling and the development of cardiac dysfunction in type 2 diabetic db/db mice. Methods: Thereto, 14-wks-old male db/db mice and non-diabetic db/+ mice received vehicle or angiotensin II (AngII) for 4 wks to induce mild hypertension (n = 9-10 per group). Left ventricular (LV) function was assessed by serial echocardiography and during a dobutamine stress test. LV tissue was subjected to molecular and (immuno) histochemical analysis to assess effects on hypertrophy, fibrosis and inflammation. Results: Vehicle-treated diabetic mice neither displayed marked myocardial structural remodeling nor cardiac dysfunction. AngII-treatment did not affect body weight and fasting glucose levels, and induced a comparable increase in blood pressure in diabetic and control mice. Nonetheless, AngII-induced LV hypertrophy was significantly more pronounced in diabetic than in control mice as assessed by LV mass (increase +51% and +34%, respectively, p
U2 - 10.1371/journal.pone.0085078
DO - 10.1371/journal.pone.0085078
M3 - Article
SN - 1932-6203
VL - 9
JO - PLOS ONE
JF - PLOS ONE
IS - 1
M1 - e85078
ER -