Human intestinal microbiota composition is associated with local and systemic inflammation in obesity.

F.J. Verdam, S. Fuentes, C. de Jonge, E.G. Zoetendal, R. Erbil, J.W. Greve, W.A. Buurman, W.M. de Vos, S.S.M. Rensen*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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OBJECTIVE: Intestinal microbiota have been suggested to contribute to development of obesity, but the mechanism remains elusive. We relationship between microbiota composition, intestinal permeability, inflammation in non-obese and obese subjects. DESIGN AND METHODS: Fecal microbiota composition of 28 subjects (BMI 18.6-60.3kg/m2 ) was analyzed phylogenetic profiling microarray. Fecal calprotectin and plasma C- protein levels were determined to evaluate intestinal and systemic Furthermore, HbA1c , and plasma levels of transaminases and lipids were Gastroduodenal, small intestinal, and colonic permeability were assessed multi-saccharide test. RESULTS: Based on microbiota composition, the population segregated into two clusters with predominantly obese (15/19) exclusively non-obese (9/9) subjects. Whereas intestinal permeability differ between clusters, the obese cluster showed reduced bacterial decreased Bacteroidetes/Firmicutes ratio, and an increased abundance of pro-inflammatory Proteobacteria. Interestingly, fecal calprotectin was detectable in subjects within the obese microbiota cluster (n=8/19, Plasma C-reactive protein was also increased in these subjects correlated with the Bacteroidetes/Firmicutes ratio (rs =-0.41, p=0.03). CONCLUSIONS: Intestinal microbiota alterations in obese subjects are with local and systemic inflammation, suggesting that the obesity- microbiota composition has a pro-inflammatory effect.
Original languageEnglish
Pages (from-to)E607-E615
Issue number12
Publication statusPublished - 1 Jan 2013


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