Abstract
Aim: Homocysteine (Hcy) is a sensitive marker of one-carbon metabolism. Higher Hcy levels have been associated with global DNA hypomethylation. We investigated the association between plasma Hcy and epigenome-wide DNA methylation in leukocytes. Methods: Methylation was measured using Illumina 450 k arrays in 2035 individuals from six cohorts. Hcy-associated differentially methylated positions and regions were identified using meta-analysis. Results: Three differentially methylated positions cg21607669 (SLC27A1), cg26382848 (AJUBA) and cg10701000 (KCNMA1) at chromosome 19, 14 and 10, respectively, were significantly associated with Hcy. In addition, we identified 68 Hcy-associated differentially methylated regions, the most significant of which was a 1.8-kb spanning domain (TNXB/ATF6B) at chromosome 6. Conclusion: We identified novel epigenetic loci associated with Hcy levels, of which specific role needs to be further validated.
Original language | English |
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Pages (from-to) | 1403-1422 |
Number of pages | 20 |
Journal | Epigenomics |
Volume | 9 |
Issue number | 11 |
DOIs | |
Publication status | Published - Nov 2017 |
Keywords
- 450 k array
- association
- DMP
- DMR
- DNA methylation
- epigenome-wide analysis
- homocysteine
- meta-analysis
- RANDOMIZED CONTROLLED-TRIAL
- ESTROGEN-RECEPTOR-ALPHA
- SMOOTH-MUSCLE-CELLS
- PLASMA HOMOCYSTEINE
- S-ADENOSYLHOMOCYSTEINE
- GENOME-WIDE
- FOLIC-ACID
- VASCULAR-DISEASE
- LIM PROTEIN
- MYOCARDIAL-INFARCTION