TY - UNPB
T1 - Hippocampal ?CaMKII dopaminylation promotes synaptic-to-nuclear signaling and memory formation
AU - Stewart, Andrew F
AU - Fulton, Sasha L
AU - Durand-de Cuttoli, Romain
AU - Thompson, Robert E
AU - Chen, Peng-Jen
AU - Brindley, Elizabeth
AU - Cetin, Bulent
AU - Farrelly, Lorna A
AU - Futamura, Rita
AU - Claypool, Sarah
AU - Bastle, Ryan M
AU - Di Salvo, Giuseppina
AU - Peralta, Christopher
AU - Molina, Henrik
AU - Baljinnyam, Erdene
AU - Marro, Samuele G
AU - Russo, Scott J
AU - DeVita, Robert J
AU - Muir, Tom W
AU - Maze, Ian
PY - 2024/9/24
Y1 - 2024/9/24
N2 - Protein monoaminylation is a class of posttranslational modification (PTM) that contributes to transcription, physiology and behavior. While recent analyses have focused on histones as critical substrates of monoaminylation, the broader repertoire of monoaminylated proteins in brain remains unclear. Here, we report the development/implementation of a chemical probe for the bioorthogonal labeling, enrichment and proteomics-based detection of dopaminylated proteins in brain. We identified 1,557 dopaminylated proteins - many synaptic - including ?CaMKII, which mediates Ca -dependent cellular signaling and hippocampal-dependent memory. We found that ?CaMKII dopaminylation is largely synaptic and mediates synaptic-to-nuclear signaling, neuronal gene expression and intrinsic excitability, and contextual memory. These results indicate a critical role for synaptic dopaminylation in adaptive brain plasticity, and may suggest roles for these phenomena in pathologies associated with altered monoaminergic signaling.
AB - Protein monoaminylation is a class of posttranslational modification (PTM) that contributes to transcription, physiology and behavior. While recent analyses have focused on histones as critical substrates of monoaminylation, the broader repertoire of monoaminylated proteins in brain remains unclear. Here, we report the development/implementation of a chemical probe for the bioorthogonal labeling, enrichment and proteomics-based detection of dopaminylated proteins in brain. We identified 1,557 dopaminylated proteins - many synaptic - including ?CaMKII, which mediates Ca -dependent cellular signaling and hippocampal-dependent memory. We found that ?CaMKII dopaminylation is largely synaptic and mediates synaptic-to-nuclear signaling, neuronal gene expression and intrinsic excitability, and contextual memory. These results indicate a critical role for synaptic dopaminylation in adaptive brain plasticity, and may suggest roles for these phenomena in pathologies associated with altered monoaminergic signaling.
U2 - 10.1101/2024.09.19.613951
DO - 10.1101/2024.09.19.613951
M3 - Preprint
BT - Hippocampal ?CaMKII dopaminylation promotes synaptic-to-nuclear signaling and memory formation
ER -