High-throughput measurement of human platelet aggregation under flow: application in hemostasis and beyond

Sanne L. N. Brouns*, Johanna P. van Geffen, Johan W. M. Heemskerk

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

19 Citations (Web of Science)

Abstract

In recent years, considerable progress has been made in understanding the mechanisms involved in platelet activation during hemostasis and thrombosis. Parallel-plate flow chambers and other microfluidic devices have markedly contributed to this insight. Conversely, such flow devices are now increasingly used to monitor the combined processes of platelet aggregation, thrombus formation, and coagulation in human blood. Currently, by combining microspotting and multi-color fluorescence microscopy, this technology offers the capability of high-throughput measurement of platelet activation processes, even in small blood samples. Here we review the potential of flow chamber devices for complex (multiparameter) platelet and coagulation phenotyping, focusing on patients with (genetic) platelet- or coagulation-based bleeding disorders as well as monitoring of antithrombotic medication. Animal studies are not discussed.
Original languageEnglish
Pages (from-to)662-669
Number of pages8
JournalPlatelets
Volume29
Issue number7
DOIs
Publication statusPublished - 1 Jan 2018

Keywords

  • Adhesion
  • fibrin procoagulant
  • thrombus
  • EXPERIMENTAL ARTERIAL THROMBOGENESIS
  • DEPENDENT THROMBUS FORMATION
  • VON-WILLEBRAND-DISEASE
  • SHEAR-RATE
  • GLYCOPROTEIN-VI
  • PROCOAGULANT ACTIVITY
  • SCOTT SYNDROME
  • WHOLE-BLOOD
  • EX-VIVO
  • ATHEROSCLEROTIC PLAQUES

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