High-throughput data integration of RNA-miRNA-circRNA reveals novel insights into mechanisms of benzo[a]pyrene-induced carcinogenicity

Florian Caiment*, Stan Gaj, Sandra Claessen, Jos Kleinjans

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

99 Citations (Web of Science)

Abstract

The chain of events leading from a toxic compound exposure to carcinogenicity is still barely understood. With the emergence of high-throughput sequencing, it is now possible to discover many different biological components simultaneously. Using two different RNA libraries, we sequenced the complete transcriptome of human HepG2 liver cells exposed to benzo[a]pyrene, a potent human carcinogen, across six time points. Data were integrated in order to reveal novel complex chemical-gene interactions. Notably, we hypothesized that the inhibition of MGMT, a DNA damage response enzyme, by the over-expressed miR-181a-1 3p induced by BaP, may lead to liver cancer over time.
Original languageEnglish
Pages (from-to)2525-2534
JournalNucleic Acids Research
Volume43
Issue number5
DOIs
Publication statusPublished - 11 Mar 2015

Cite this