High-resolution magnetic resonance imaging quantitatively detects individual pancreatic islets

S. Lamprianou, R. Immonen, C.I. Nabuurs, A. Gjinovci, L. Vinet, X.C. Montet, R. Gruetter, P. Meda

Research output: Contribution to journalArticleAcademicpeer-review

23 Citations (Scopus)

Abstract

OBJECTIVE: We studied whether manganese-enhanced high-field magnetic resonance (MR) imaging (MEHFMRI) could quantitatively detect individual islets in situ and in vivo and evaluate changes in a model of experimental diabetes. RESEARCH DESIGN AND METHODS: Whole pancreata from untreated (n = 3), MnCl(2) and glucose-injected mice (n = 6), and mice injected with either streptozotocin (STZ; n = 4) or citrate buffer (n = 4) were imaged ex vivo for unambiguous evaluation of islets. Exteriorized pancreata of MnCl(2) and glucose-injected mice (n = 6) were imaged in vivo to directly visualize the gland and minimize movements. In all cases, MR images were acquired in a 14.1 Tesla scanner and correlated with the corresponding (immuno)histological sections. RESULTS: In ex vivo experiments, MEHFMRI distinguished different pancreatic tissues and evaluated the relative abundance of islets in the pancreata of normoglycemic mice. MEHFMRI also detected a significant decrease in the numerical and volume density of islets in STZ-injected mice. However, in the latter measurements the loss of beta-cells was undervalued under the conditions tested. The experiments on the externalized pancreata confirmed that MEHFMRI could visualize native individual islets in living, anesthetized mice. CONCLUSIONS: Data show that MEHFMRI quantitatively visualizes individual islets in the intact mouse pancreas, both ex vivo and in vivo.
Original languageEnglish
Pages (from-to)2853-2860
Number of pages8
JournalDiabetes
Volume60
Issue number11
DOIs
Publication statusPublished - Nov 2011

Keywords

  • BETA-CELL MASS
  • IN-VIVO
  • TRANSPLANTATION
  • STREPTOZOTOCIN
  • MOUSE
  • MICE
  • NANOPARTICLES
  • MOLECULES
  • HYPERGLYCEMIA
  • INFLAMMATION

Cite this

Lamprianou, S., Immonen, R., Nabuurs, C. I., Gjinovci, A., Vinet, L., Montet, X. C., ... Meda, P. (2011). High-resolution magnetic resonance imaging quantitatively detects individual pancreatic islets. Diabetes, 60(11), 2853-2860. https://doi.org/10.2337/db11-0726
Lamprianou, S. ; Immonen, R. ; Nabuurs, C.I. ; Gjinovci, A. ; Vinet, L. ; Montet, X.C. ; Gruetter, R. ; Meda, P. / High-resolution magnetic resonance imaging quantitatively detects individual pancreatic islets. In: Diabetes. 2011 ; Vol. 60, No. 11. pp. 2853-2860.
@article{bc87174fd7714723815ad6e18e821244,
title = "High-resolution magnetic resonance imaging quantitatively detects individual pancreatic islets",
abstract = "OBJECTIVE: We studied whether manganese-enhanced high-field magnetic resonance (MR) imaging (MEHFMRI) could quantitatively detect individual islets in situ and in vivo and evaluate changes in a model of experimental diabetes. RESEARCH DESIGN AND METHODS: Whole pancreata from untreated (n = 3), MnCl(2) and glucose-injected mice (n = 6), and mice injected with either streptozotocin (STZ; n = 4) or citrate buffer (n = 4) were imaged ex vivo for unambiguous evaluation of islets. Exteriorized pancreata of MnCl(2) and glucose-injected mice (n = 6) were imaged in vivo to directly visualize the gland and minimize movements. In all cases, MR images were acquired in a 14.1 Tesla scanner and correlated with the corresponding (immuno)histological sections. RESULTS: In ex vivo experiments, MEHFMRI distinguished different pancreatic tissues and evaluated the relative abundance of islets in the pancreata of normoglycemic mice. MEHFMRI also detected a significant decrease in the numerical and volume density of islets in STZ-injected mice. However, in the latter measurements the loss of beta-cells was undervalued under the conditions tested. The experiments on the externalized pancreata confirmed that MEHFMRI could visualize native individual islets in living, anesthetized mice. CONCLUSIONS: Data show that MEHFMRI quantitatively visualizes individual islets in the intact mouse pancreas, both ex vivo and in vivo.",
keywords = "BETA-CELL MASS, IN-VIVO, TRANSPLANTATION, STREPTOZOTOCIN, MOUSE, MICE, NANOPARTICLES, MOLECULES, HYPERGLYCEMIA, INFLAMMATION",
author = "S. Lamprianou and R. Immonen and C.I. Nabuurs and A. Gjinovci and L. Vinet and X.C. Montet and R. Gruetter and P. Meda",
year = "2011",
month = "11",
doi = "10.2337/db11-0726",
language = "English",
volume = "60",
pages = "2853--2860",
journal = "Diabetes",
issn = "0012-1797",
publisher = "American Diabetes Association",
number = "11",

}

Lamprianou, S, Immonen, R, Nabuurs, CI, Gjinovci, A, Vinet, L, Montet, XC, Gruetter, R & Meda, P 2011, 'High-resolution magnetic resonance imaging quantitatively detects individual pancreatic islets', Diabetes, vol. 60, no. 11, pp. 2853-2860. https://doi.org/10.2337/db11-0726

High-resolution magnetic resonance imaging quantitatively detects individual pancreatic islets. / Lamprianou, S.; Immonen, R.; Nabuurs, C.I.; Gjinovci, A.; Vinet, L.; Montet, X.C.; Gruetter, R.; Meda, P.

In: Diabetes, Vol. 60, No. 11, 11.2011, p. 2853-2860.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - High-resolution magnetic resonance imaging quantitatively detects individual pancreatic islets

AU - Lamprianou, S.

AU - Immonen, R.

AU - Nabuurs, C.I.

AU - Gjinovci, A.

AU - Vinet, L.

AU - Montet, X.C.

AU - Gruetter, R.

AU - Meda, P.

PY - 2011/11

Y1 - 2011/11

N2 - OBJECTIVE: We studied whether manganese-enhanced high-field magnetic resonance (MR) imaging (MEHFMRI) could quantitatively detect individual islets in situ and in vivo and evaluate changes in a model of experimental diabetes. RESEARCH DESIGN AND METHODS: Whole pancreata from untreated (n = 3), MnCl(2) and glucose-injected mice (n = 6), and mice injected with either streptozotocin (STZ; n = 4) or citrate buffer (n = 4) were imaged ex vivo for unambiguous evaluation of islets. Exteriorized pancreata of MnCl(2) and glucose-injected mice (n = 6) were imaged in vivo to directly visualize the gland and minimize movements. In all cases, MR images were acquired in a 14.1 Tesla scanner and correlated with the corresponding (immuno)histological sections. RESULTS: In ex vivo experiments, MEHFMRI distinguished different pancreatic tissues and evaluated the relative abundance of islets in the pancreata of normoglycemic mice. MEHFMRI also detected a significant decrease in the numerical and volume density of islets in STZ-injected mice. However, in the latter measurements the loss of beta-cells was undervalued under the conditions tested. The experiments on the externalized pancreata confirmed that MEHFMRI could visualize native individual islets in living, anesthetized mice. CONCLUSIONS: Data show that MEHFMRI quantitatively visualizes individual islets in the intact mouse pancreas, both ex vivo and in vivo.

AB - OBJECTIVE: We studied whether manganese-enhanced high-field magnetic resonance (MR) imaging (MEHFMRI) could quantitatively detect individual islets in situ and in vivo and evaluate changes in a model of experimental diabetes. RESEARCH DESIGN AND METHODS: Whole pancreata from untreated (n = 3), MnCl(2) and glucose-injected mice (n = 6), and mice injected with either streptozotocin (STZ; n = 4) or citrate buffer (n = 4) were imaged ex vivo for unambiguous evaluation of islets. Exteriorized pancreata of MnCl(2) and glucose-injected mice (n = 6) were imaged in vivo to directly visualize the gland and minimize movements. In all cases, MR images were acquired in a 14.1 Tesla scanner and correlated with the corresponding (immuno)histological sections. RESULTS: In ex vivo experiments, MEHFMRI distinguished different pancreatic tissues and evaluated the relative abundance of islets in the pancreata of normoglycemic mice. MEHFMRI also detected a significant decrease in the numerical and volume density of islets in STZ-injected mice. However, in the latter measurements the loss of beta-cells was undervalued under the conditions tested. The experiments on the externalized pancreata confirmed that MEHFMRI could visualize native individual islets in living, anesthetized mice. CONCLUSIONS: Data show that MEHFMRI quantitatively visualizes individual islets in the intact mouse pancreas, both ex vivo and in vivo.

KW - BETA-CELL MASS

KW - IN-VIVO

KW - TRANSPLANTATION

KW - STREPTOZOTOCIN

KW - MOUSE

KW - MICE

KW - NANOPARTICLES

KW - MOLECULES

KW - HYPERGLYCEMIA

KW - INFLAMMATION

U2 - 10.2337/db11-0726

DO - 10.2337/db11-0726

M3 - Article

VL - 60

SP - 2853

EP - 2860

JO - Diabetes

JF - Diabetes

SN - 0012-1797

IS - 11

ER -

Lamprianou S, Immonen R, Nabuurs CI, Gjinovci A, Vinet L, Montet XC et al. High-resolution magnetic resonance imaging quantitatively detects individual pancreatic islets. Diabetes. 2011 Nov;60(11):2853-2860. https://doi.org/10.2337/db11-0726