TY - UNPB
T1 - High-resolution bladder morphology in the intact mouse bladder
T2 - an intravital two-photon study
AU - Schueth, Anna
AU - Theelen, Thomas L.
AU - Foulqier, Sebastien
AU - van Koeveringe, Gommert A.
AU - van Zandvoort, Marc A. M. J.
N1 - Authors retain copyright and choose from several distribution/reuse options under which to make the article available (CC BY, CC BY-NC, CC BY-ND, CC BY-NC-ND, CC0, or no reuse).
PY - 2018/12/31
Y1 - 2018/12/31
N2 - We developed a platform for intravital bladder two-photon microscopy (TPM) in healthy wild-type mice. It was our aim to make intravital bladder TPM simple and reproducible for researchers with access to TPM. In this study, the intact murine bladder was examined by means of autofluorescence (AF), second-harmonic generation (SHG), and different i.v. injected fluorescent dyes. All bladder layers with containing structures, such as cells, nerves, and vessels were detected in different colour-coded spectral channels, and shown in high-resolution images and real-time movies. The presented method opens up avenues for many future studies and applications, such as to reveal mechanisms and physiology in the natural state of the bladder. Researcher can use intravital bladder TPM to investigate events, such as migration and metabolism of (inflammatory) cells, nanoparticle uptake, or micro contractions in response to stimuli.
AB - We developed a platform for intravital bladder two-photon microscopy (TPM) in healthy wild-type mice. It was our aim to make intravital bladder TPM simple and reproducible for researchers with access to TPM. In this study, the intact murine bladder was examined by means of autofluorescence (AF), second-harmonic generation (SHG), and different i.v. injected fluorescent dyes. All bladder layers with containing structures, such as cells, nerves, and vessels were detected in different colour-coded spectral channels, and shown in high-resolution images and real-time movies. The presented method opens up avenues for many future studies and applications, such as to reveal mechanisms and physiology in the natural state of the bladder. Researcher can use intravital bladder TPM to investigate events, such as migration and metabolism of (inflammatory) cells, nanoparticle uptake, or micro contractions in response to stimuli.
KW - cell-biology
U2 - 10.1101/508879
DO - 10.1101/508879
M3 - Preprint
BT - High-resolution bladder morphology in the intact mouse bladder
PB - Cold Spring Harbor Laboratory - bioRxiv
ER -