hFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue

Sven Hildebrand*, Anna Schueth, Klaus von Wangenheim, Christian Mattheyer, Francesco Pampaloni, Hansjürgen Bratzke, Alard F. Roebroeck, Ralf A.W. Galuske

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Web of Science)

Abstract

Here, we describe a new immersion-based clearing method suitable for optical clearing of thick adult human brain samples while preserving its lipids and lipophilic labels such as 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI). This clearing procedure is simple, easy to implement, and allowed for clearing of 5 mm thick human brain tissue samples within 12 days. Furthermore, we show for the first time the advantageous effect of the Periodate-Lysine-Paraformaldehyde (PLP) fixation as compared to the more commonly used 4% paraformaldehyde (PFA) on clearing performance.

Original languageEnglish
Article number9950
Number of pages10
JournalScientific Reports
Volume10
Issue number1
DOIs
Publication statusPublished - 19 Jun 2020

Keywords

  • MICROSCOPY

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