TY - JOUR
T1 - hFRUIT
T2 - An optimized agent for optical clearing of DiI-stained adult human brain tissue
AU - Hildebrand, Sven
AU - Schueth, Anna
AU - Wangenheim, Klaus von
AU - Mattheyer, Christian
AU - Pampaloni, Francesco
AU - Bratzke, Hansjürgen
AU - Roebroeck, Alard F.
AU - Galuske, Ralf A.W.
PY - 2020/6/19
Y1 - 2020/6/19
N2 - Here, we describe a new immersion-based clearing method suitable for optical clearing of thick adult human brain samples while preserving its lipids and lipophilic labels such as 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI). This clearing procedure is simple, easy to implement, and allowed for clearing of 5 mm thick human brain tissue samples within 12 days. Furthermore, we show for the first time the advantageous effect of the Periodate-Lysine-Paraformaldehyde (PLP) fixation as compared to the more commonly used 4% paraformaldehyde (PFA) on clearing performance.
AB - Here, we describe a new immersion-based clearing method suitable for optical clearing of thick adult human brain samples while preserving its lipids and lipophilic labels such as 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI). This clearing procedure is simple, easy to implement, and allowed for clearing of 5 mm thick human brain tissue samples within 12 days. Furthermore, we show for the first time the advantageous effect of the Periodate-Lysine-Paraformaldehyde (PLP) fixation as compared to the more commonly used 4% paraformaldehyde (PFA) on clearing performance.
KW - MICROSCOPY
U2 - 10.1038/s41598-020-66999-3
DO - 10.1038/s41598-020-66999-3
M3 - Article
C2 - 32561795
VL - 10
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
IS - 1
M1 - 9950
ER -