hFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue

Sven Hildebrand; Anna Schueth; Klaus von Wangenheim; Christian Mattheyer; Francesco Pampaloni; Hansjürgen Bratzke; Alard F. Roebroeck; Ralf A.W. Galuske

doi:10.1038/s41598-020-66999-3

hFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue

Sven Hildebrand^*, Anna Schueth, Klaus von Wangenheim, Christian Mattheyer, Francesco Pampaloni, Hansjürgen Bratzke, Alard F. Roebroeck, Ralf A.W. Galuske

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Here, we describe a new immersion-based clearing method suitable for optical clearing of thick adult human brain samples while preserving its lipids and lipophilic labels such as 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI). This clearing procedure is simple, easy to implement, and allowed for clearing of 5 mm thick human brain tissue samples within 12 days. Furthermore, we show for the first time the advantageous effect of the Periodate-Lysine-Paraformaldehyde (PLP) fixation as compared to the more commonly used 4% paraformaldehyde (PFA) on clearing performance.

Original language	English
Article number	9950
Number of pages	10
Journal	Scientific Reports
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41598-020-66999-3
Publication status	Published - 19 Jun 2020

Keywords

MICROSCOPY

Access to Document

10.1038/s41598-020-66999-3Licence: CC BY

Cite this

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title = "hFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue",

abstract = "Here, we describe a new immersion-based clearing method suitable for optical clearing of thick adult human brain samples while preserving its lipids and lipophilic labels such as 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI). This clearing procedure is simple, easy to implement, and allowed for clearing of 5 mm thick human brain tissue samples within 12 days. Furthermore, we show for the first time the advantageous effect of the Periodate-Lysine-Paraformaldehyde (PLP) fixation as compared to the more commonly used 4% paraformaldehyde (PFA) on clearing performance.",

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doi = "10.1038/s41598-020-66999-3",

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T2 - An optimized agent for optical clearing of DiI-stained adult human brain tissue

AU - Hildebrand, Sven

AU - Schueth, Anna

AU - Wangenheim, Klaus von

AU - Mattheyer, Christian

AU - Pampaloni, Francesco

AU - Bratzke, Hansjürgen

AU - Roebroeck, Alard F.

AU - Galuske, Ralf A.W.

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AB - Here, we describe a new immersion-based clearing method suitable for optical clearing of thick adult human brain samples while preserving its lipids and lipophilic labels such as 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI). This clearing procedure is simple, easy to implement, and allowed for clearing of 5 mm thick human brain tissue samples within 12 days. Furthermore, we show for the first time the advantageous effect of the Periodate-Lysine-Paraformaldehyde (PLP) fixation as compared to the more commonly used 4% paraformaldehyde (PFA) on clearing performance.

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