Hemolysis compromises nitric oxide-dependent vasodilatory responses in patients undergoing major cardiovascular surgery.

S.J.P. Hanssen, M.C.G. van de Poll, A.J. Houben, I.C. Vermeulen Windsant, M.G. Snoeijs, O. Bekers, W.A. Buurman, M.J.H.M. Jacobs

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

Background The hemolytic products cell-free oxyhemoglobin (FHb) and arginase-1 reduce nitric oxide (NO) bioavailability by scavenging NO and by degrading the NO precursor arginine to ornithine, respectively. In this study we evaluated the relevance of hemolysis to NO-dependent blood flow in patients undergoing cardiovascular surgery.Methods Plasma FHb, arginase-1, and amino acid concentrations were measured perioperatively. Forearm blood flow (FBF) responses to the intra-arterial administered NO-donor sodium nitroprusside (SNP) and the endothelium-dependent vasodilator acetylcholine (ACh) were measured by venous occlusion plethysmography.Results When peak values plasma FHb and arginase-1 were found, vascular dilatation to SNP, but not ACh, was significantly reduced compared with 1 day postoperatively, when FHb had returned to baseline levels (p < 0.05). Interestingly, plasma FHb concentration was inversely correlated to FBF responses to SNP (r -0.93, p < 0.001). In contrast, the increase in arginase-1 was not biologically relevant as the ratio of plasma arginine to ornithine remained constant.Conclusion We conclude that hemolysis with concomitant release of FHb during cardiovascular surgery is associated with impaired NO-dependent forearm blood flow.
Original languageEnglish
Pages (from-to)255-261
Number of pages7
JournalThoracic and Cardiovascular Surgeon
Volume60
Issue number4
DOIs
Publication statusPublished - Jun 2012

Keywords

  • cardiopulmonary bypass
  • nitric oxide
  • vascular science (biochemistry pharmacology pathology)
  • SICKLE-CELL-DISEASE
  • AORTIC-SURGERY
  • HEMOGLOBIN
  • INJURY
  • BIOAVAILABILITY
  • HUMANS
  • PLASMA
  • SYSTEM
  • REPAIR

Cite this

@article{72c97b5f54ec4c4286d30984ae352e80,
title = "Hemolysis compromises nitric oxide-dependent vasodilatory responses in patients undergoing major cardiovascular surgery.",
abstract = "Background The hemolytic products cell-free oxyhemoglobin (FHb) and arginase-1 reduce nitric oxide (NO) bioavailability by scavenging NO and by degrading the NO precursor arginine to ornithine, respectively. In this study we evaluated the relevance of hemolysis to NO-dependent blood flow in patients undergoing cardiovascular surgery.Methods Plasma FHb, arginase-1, and amino acid concentrations were measured perioperatively. Forearm blood flow (FBF) responses to the intra-arterial administered NO-donor sodium nitroprusside (SNP) and the endothelium-dependent vasodilator acetylcholine (ACh) were measured by venous occlusion plethysmography.Results When peak values plasma FHb and arginase-1 were found, vascular dilatation to SNP, but not ACh, was significantly reduced compared with 1 day postoperatively, when FHb had returned to baseline levels (p < 0.05). Interestingly, plasma FHb concentration was inversely correlated to FBF responses to SNP (r -0.93, p < 0.001). In contrast, the increase in arginase-1 was not biologically relevant as the ratio of plasma arginine to ornithine remained constant.Conclusion We conclude that hemolysis with concomitant release of FHb during cardiovascular surgery is associated with impaired NO-dependent forearm blood flow.",
keywords = "cardiopulmonary bypass, nitric oxide, vascular science (biochemistry pharmacology pathology), SICKLE-CELL-DISEASE, AORTIC-SURGERY, HEMOGLOBIN, INJURY, BIOAVAILABILITY, HUMANS, PLASMA, SYSTEM, REPAIR",
author = "S.J.P. Hanssen and {van de Poll}, M.C.G. and A.J. Houben and {Vermeulen Windsant}, I.C. and M.G. Snoeijs and O. Bekers and W.A. Buurman and M.J.H.M. Jacobs",
year = "2012",
month = "6",
doi = "10.1055/s-0031-1299571",
language = "English",
volume = "60",
pages = "255--261",
journal = "Thoracic and Cardiovascular Surgeon",
issn = "0171-6425",
publisher = "Georg Thieme Verlag",
number = "4",

}

Hemolysis compromises nitric oxide-dependent vasodilatory responses in patients undergoing major cardiovascular surgery. / Hanssen, S.J.P.; van de Poll, M.C.G.; Houben, A.J.; Vermeulen Windsant, I.C.; Snoeijs, M.G.; Bekers, O.; Buurman, W.A.; Jacobs, M.J.H.M.

In: Thoracic and Cardiovascular Surgeon, Vol. 60, No. 4, 06.2012, p. 255-261.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Hemolysis compromises nitric oxide-dependent vasodilatory responses in patients undergoing major cardiovascular surgery.

AU - Hanssen, S.J.P.

AU - van de Poll, M.C.G.

AU - Houben, A.J.

AU - Vermeulen Windsant, I.C.

AU - Snoeijs, M.G.

AU - Bekers, O.

AU - Buurman, W.A.

AU - Jacobs, M.J.H.M.

PY - 2012/6

Y1 - 2012/6

N2 - Background The hemolytic products cell-free oxyhemoglobin (FHb) and arginase-1 reduce nitric oxide (NO) bioavailability by scavenging NO and by degrading the NO precursor arginine to ornithine, respectively. In this study we evaluated the relevance of hemolysis to NO-dependent blood flow in patients undergoing cardiovascular surgery.Methods Plasma FHb, arginase-1, and amino acid concentrations were measured perioperatively. Forearm blood flow (FBF) responses to the intra-arterial administered NO-donor sodium nitroprusside (SNP) and the endothelium-dependent vasodilator acetylcholine (ACh) were measured by venous occlusion plethysmography.Results When peak values plasma FHb and arginase-1 were found, vascular dilatation to SNP, but not ACh, was significantly reduced compared with 1 day postoperatively, when FHb had returned to baseline levels (p < 0.05). Interestingly, plasma FHb concentration was inversely correlated to FBF responses to SNP (r -0.93, p < 0.001). In contrast, the increase in arginase-1 was not biologically relevant as the ratio of plasma arginine to ornithine remained constant.Conclusion We conclude that hemolysis with concomitant release of FHb during cardiovascular surgery is associated with impaired NO-dependent forearm blood flow.

AB - Background The hemolytic products cell-free oxyhemoglobin (FHb) and arginase-1 reduce nitric oxide (NO) bioavailability by scavenging NO and by degrading the NO precursor arginine to ornithine, respectively. In this study we evaluated the relevance of hemolysis to NO-dependent blood flow in patients undergoing cardiovascular surgery.Methods Plasma FHb, arginase-1, and amino acid concentrations were measured perioperatively. Forearm blood flow (FBF) responses to the intra-arterial administered NO-donor sodium nitroprusside (SNP) and the endothelium-dependent vasodilator acetylcholine (ACh) were measured by venous occlusion plethysmography.Results When peak values plasma FHb and arginase-1 were found, vascular dilatation to SNP, but not ACh, was significantly reduced compared with 1 day postoperatively, when FHb had returned to baseline levels (p < 0.05). Interestingly, plasma FHb concentration was inversely correlated to FBF responses to SNP (r -0.93, p < 0.001). In contrast, the increase in arginase-1 was not biologically relevant as the ratio of plasma arginine to ornithine remained constant.Conclusion We conclude that hemolysis with concomitant release of FHb during cardiovascular surgery is associated with impaired NO-dependent forearm blood flow.

KW - cardiopulmonary bypass

KW - nitric oxide

KW - vascular science (biochemistry pharmacology pathology)

KW - SICKLE-CELL-DISEASE

KW - AORTIC-SURGERY

KW - HEMOGLOBIN

KW - INJURY

KW - BIOAVAILABILITY

KW - HUMANS

KW - PLASMA

KW - SYSTEM

KW - REPAIR

U2 - 10.1055/s-0031-1299571

DO - 10.1055/s-0031-1299571

M3 - Article

VL - 60

SP - 255

EP - 261

JO - Thoracic and Cardiovascular Surgeon

JF - Thoracic and Cardiovascular Surgeon

SN - 0171-6425

IS - 4

ER -