Hearts of surviving MLP-KO mice show transient changes of intracellular calcium handling

Peter Kemecsei, Zsuzsanna Miklos, Tamas Biro, Rita Marincsak, Balazs I. Toth, Edina Komlodi-Pasztor, Eniko Barnucz, Eva Mirk, Ger J. van der Vusse, Laszlo Ligeti, Tamas Ivanics*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Web of Science)


The muscle Lim protein knock-out (MLP-KO) mouse model is extensively used for studying the pathophysiology of dilated cardiomyopathy. However, explanation is lacking for the observed long survival of the diseased mice which develop until adulthood despite the gene defect, which theoretically predestines them to early death due to heart failure. We hypothesized that adaptive changes of cardiac intracellular calcium (Ca (i) (2+) ) handling might explain the phenomenon. In order to study the progression of changes in cardiac function and Ca (i) (2+) cycling, myocardial Ca (i) (2+) -transients recorded by Indo-1 surface fluorometry were assessed with concomitant measurement of hemodynamic performance in isolated Langendorff-perfused hearts of 3- and 9-month old MLP-KO animals. Hearts were challenged with beta-agonist isoproterenol and the sarcoplasmic reticular Ca2+-ATPase (SERCA2a) inhibitor cyclopiazonic acid (CPA). Cardiac mRNA content and levels of key Ca2+ handling proteins were also measured. A decline in lusitropic function was observed in 3-month old, but not in 9-month old MLP-KO mice under unchallenged conditions. beta-adrenergic responses to isoproterenol were similar in all the studied groups. The CPA induced an increase in end-diastolic Ca (i) (2+) -level and a decrease in Ca2+-sequestration capacity in 3-month old MLP-KO mice compared to age-matched controls. This unfavorable condition was absent at 9 months of age. SERCA2a expression was lower in 3-month old MLP-KO than in the corresponding controls and in 9-month old MLP-KO hearts. Our results show time-related recovery of hemodynamic function and an age-dependent compensatory upregulation of Ca (i) (2+) handling in hearts of MLP-KO mice, which most likely involve the normalization of the expression of SERCA2a in the affected hearts.
Original languageEnglish
Pages (from-to)251-260
JournalMolecular and Cellular Biochemistry
Issue number1-2
Publication statusPublished - Sept 2010


  • Ca(i)(2+) handling
  • Isolated hearts
  • MLP-KO

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