Gold Sputtered Fiducial Markers for Combined Secondary Ion Mass Spectrometry and MALDI Imaging of Tissue Samples

Nina Ogrinc Potocnik; Karolina Skraskova; Bryn Flinders; Primoz Pelicon; Ron M. A. Heeren

doi:10.1021/ac500308s

Gold Sputtered Fiducial Markers for Combined Secondary Ion Mass Spectrometry and MALDI Imaging of Tissue Samples

Nina Ogrinc Potocnik, Karolina Skraskova, Bryn Flinders, Primoz Pelicon, Ron M. A. Heeren^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Mass spectrometry imaging (MSI) is a label free technique capable of providing simultaneous identification and localization of biomolecules. A multimodal approach is required that allows for the study of the complexity of biological tissue samples to overcome the limitations of a single MSI technique. Secondary ion mass spectrometry (SIMS) allows for high spatial resolution imaging while matrix-assisted laser desorption (MALDI) offers a significantly wider mass range. The combination of coregistered SIMS and MALDI images results in detailed and unique biomolecular information. In this Technical Note, we describe how gold sputtered/implanted fiducial markers (FM) are created and can be used to ensure a proper overlay and coregistration of the two-dimensional images provided by the two MSI modalities.

Original language	English
Pages (from-to)	6781-6785
Journal	Analytical Chemistry
Volume	86
Issue number	14
DOIs	https://doi.org/10.1021/ac500308s
Publication status	Published - 15 Jul 2014

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10.1021/ac500308s

Cite this

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title = "Gold Sputtered Fiducial Markers for Combined Secondary Ion Mass Spectrometry and MALDI Imaging of Tissue Samples",

abstract = "Mass spectrometry imaging (MSI) is a label free technique capable of providing simultaneous identification and localization of biomolecules. A multimodal approach is required that allows for the study of the complexity of biological tissue samples to overcome the limitations of a single MSI technique. Secondary ion mass spectrometry (SIMS) allows for high spatial resolution imaging while matrix-assisted laser desorption (MALDI) offers a significantly wider mass range. The combination of coregistered SIMS and MALDI images results in detailed and unique biomolecular information. In this Technical Note, we describe how gold sputtered/implanted fiducial markers (FM) are created and can be used to ensure a proper overlay and coregistration of the two-dimensional images provided by the two MSI modalities.",

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AU - Flinders, Bryn

AU - Pelicon, Primoz

AU - Heeren, Ron M. A.

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AB - Mass spectrometry imaging (MSI) is a label free technique capable of providing simultaneous identification and localization of biomolecules. A multimodal approach is required that allows for the study of the complexity of biological tissue samples to overcome the limitations of a single MSI technique. Secondary ion mass spectrometry (SIMS) allows for high spatial resolution imaging while matrix-assisted laser desorption (MALDI) offers a significantly wider mass range. The combination of coregistered SIMS and MALDI images results in detailed and unique biomolecular information. In this Technical Note, we describe how gold sputtered/implanted fiducial markers (FM) are created and can be used to ensure a proper overlay and coregistration of the two-dimensional images provided by the two MSI modalities.

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