Gold Sputtered Fiducial Markers for Combined Secondary Ion Mass Spectrometry and MALDI Imaging of Tissue Samples

Nina Ogrinc Potocnik, Karolina Skraskova, Bryn Flinders, Primoz Pelicon, Ron M. A. Heeren*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


Mass spectrometry imaging (MSI) is a label free technique capable of providing simultaneous identification and localization of biomolecules. A multimodal approach is required that allows for the study of the complexity of biological tissue samples to overcome the limitations of a single MSI technique. Secondary ion mass spectrometry (SIMS) allows for high spatial resolution imaging while matrix-assisted laser desorption (MALDI) offers a significantly wider mass range. The combination of coregistered SIMS and MALDI images results in detailed and unique biomolecular information. In this Technical Note, we describe how gold sputtered/implanted fiducial markers (FM) are created and can be used to ensure a proper overlay and coregistration of the two-dimensional images provided by the two MSI modalities.
Original languageEnglish
Pages (from-to)6781-6785
JournalAnalytical Chemistry
Issue number14
Publication statusPublished - 15 Jul 2014

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