In recent years, the importance of the mucus layer in the colon has increasingly clear. Disturbance of the mucus layer has been implicated variety of intestinal diseases. We have recently investigated the the mucus layer in colon ischemia-reperfusion (IR). Using a newly and rat colon IR model, we showed that colon ischemia leads to mucus breakdown. This allowed intraluminal bacteria to interact with the epithelium, which was associated with an inflammatory response. found goblet cells to respond immediately by expelling their mucin the gut lumen, which flushed bacteria from the colonic crypts and rapid restoration of the mucus layer during reperfusion. Our study might why ischemic colitis tends to have favorable outcomes and can often be conservatively.