TY - JOUR
T1 - Glucose ingestion during exercise blunts exercise induced gene expression of skeletal muscle fat oxidative genes
AU - Civitarese, A.E.
AU - Hesselink, M.K.
AU - Russell, A.P.
AU - Ravussin, E.
AU - Schrauwen, P.
PY - 2005/1/1
Y1 - 2005/1/1
N2 - Ingestion of carbohydrate during exercise may blunt the stimulation of fat oxidative pathways by raising plasma insulin and glucose concentrations and lowering plasma free fatty acids (FFA) levels, thereby causing a marked shift in substrate oxidation. We investigated the effects of a single 2 hour bout of moderate-intensity exercise on the expression of key genes involved in fat and carbohydrate metabolism with or without glucose ingestion in seven healthy untrained men [22.7 +/- 0.6 y; BMI: 23.8 +/- 1.0 kg/m(2); VO2 max: 3.85 +/- 0.21 l/min]. Plasma FFA concentration increased during exercise (p<0.01) in the fasted state but remained unchanged after glucose ingestion whereas fat oxidation (indirect calorimetry) was higher in the fasted state vs. glucose feeding (p<0.05). Except for a significant decrease in the expression of pyruvate dehydrogenase kinase 4 (PDK4; p<0.05), glucose ingestion during exercise produced minimal effects on the expression of genes involved in carbohydrate utilization. However, glucose ingestion resulted in a decrease in the expression of genes involved in FA transport and oxidation (CD36, CPT-1, UCP3 and AMPK-alpha2; p<0.05). In conclusion, glucose ingestion during exercise decreases the expression of genes involved in lipid metabolism rather than increasing genes involved in carbohydrate metabolism.
AB - Ingestion of carbohydrate during exercise may blunt the stimulation of fat oxidative pathways by raising plasma insulin and glucose concentrations and lowering plasma free fatty acids (FFA) levels, thereby causing a marked shift in substrate oxidation. We investigated the effects of a single 2 hour bout of moderate-intensity exercise on the expression of key genes involved in fat and carbohydrate metabolism with or without glucose ingestion in seven healthy untrained men [22.7 +/- 0.6 y; BMI: 23.8 +/- 1.0 kg/m(2); VO2 max: 3.85 +/- 0.21 l/min]. Plasma FFA concentration increased during exercise (p<0.01) in the fasted state but remained unchanged after glucose ingestion whereas fat oxidation (indirect calorimetry) was higher in the fasted state vs. glucose feeding (p<0.05). Except for a significant decrease in the expression of pyruvate dehydrogenase kinase 4 (PDK4; p<0.05), glucose ingestion during exercise produced minimal effects on the expression of genes involved in carbohydrate utilization. However, glucose ingestion resulted in a decrease in the expression of genes involved in FA transport and oxidation (CD36, CPT-1, UCP3 and AMPK-alpha2; p<0.05). In conclusion, glucose ingestion during exercise decreases the expression of genes involved in lipid metabolism rather than increasing genes involved in carbohydrate metabolism.
U2 - 10.1152/ajpendo.00193.2005
DO - 10.1152/ajpendo.00193.2005
M3 - Article
SN - 0193-1849
VL - 289
SP - E1023-E1029
JO - American Journal of Physiology : Endocrinology and Metabolism
JF - American Journal of Physiology : Endocrinology and Metabolism
IS - 6
ER -