Genome-wide microRNA expression analysis in human placenta reveals sex-specific patterns: an ENVIRONAGE birth cohort study

Maria Tsamou; Karen Vrijens; Congrong Wang; Ellen Winckelmans; Kristof Y. Neven; Narjes Madhloum; Theo M. de Kok; Tim S. Nawrot

doi:10.1080/15592294.2020.1803467

Genome-wide microRNA expression analysis in human placenta reveals sex-specific patterns: an ENVIRONAGE birth cohort study

Maria Tsamou, Karen Vrijens, Congrong Wang, Ellen Winckelmans, Kristof Y. Neven, Narjes Madhloum, Theo M. de Kok, Tim S. Nawrot^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

There is an increasing interest in microRNAs (miRNAs) as they are of utmost importance in gene regulation at the posttranscriptional level. Sex-related susceptibility for non-communicable diseases later in life could originate in early life. Until now, no data on sex-specific miRNA expression are available for the placenta. Therefore, we investigated the difference by sex of newborn's miRNA expression in human placental tissue. Within the ENVIRONAGE birth cohort, miRNA and mRNA expression profiling was performed in 60 placentae (50% boys) using Agilent (8 x 60 K) microarrays. The distribution of chromosome locations was studied and pathway analysis of the identified sex-specific miRNAs in the placenta was carried out. Of the total 2558 miRNAs on the array, 597 miRNAs were expressed in over 70% of the samples and were included for further analyses. A total of 142 miRNAs were significantly (FDR

Original language	English
Pages (from-to)	373-388
Number of pages	16
Journal	Epigenetics
Volume	16
Issue number	4
Early online date	6 Sept 2020
DOIs	https://doi.org/10.1080/15592294.2020.1803467
Publication status	Published - 3 Apr 2021

Keywords

Placenta
m(icro)RNA array
sex-related differences
MATERNAL ASTHMA
FETAL SEX
GENE-EXPRESSION
METHYLATION
GENDER
BLOOD
METABOLISM
DIMORPHISM
CARCINOMA
HORMONE

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title = "Genome-wide microRNA expression analysis in human placenta reveals sex-specific patterns: an ENVIRONAGE birth cohort study",

abstract = "There is an increasing interest in microRNAs (miRNAs) as they are of utmost importance in gene regulation at the posttranscriptional level. Sex-related susceptibility for non-communicable diseases later in life could originate in early life. Until now, no data on sex-specific miRNA expression are available for the placenta. Therefore, we investigated the difference by sex of newborn's miRNA expression in human placental tissue. Within the ENVIRONAGE birth cohort, miRNA and mRNA expression profiling was performed in 60 placentae (50% boys) using Agilent (8 x 60 K) microarrays. The distribution of chromosome locations was studied and pathway analysis of the identified sex-specific miRNAs in the placenta was carried out. Of the total 2558 miRNAs on the array, 597 miRNAs were expressed in over 70% of the samples and were included for further analyses. A total of 142 miRNAs were significantly (FDR",

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author = "Maria Tsamou and Karen Vrijens and Congrong Wang and Ellen Winckelmans and Neven, {Kristof Y.} and Narjes Madhloum and {de Kok}, {Theo M.} and Nawrot, {Tim S.}",

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AU - Vrijens, Karen

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AU - Winckelmans, Ellen

AU - Neven, Kristof Y.

AU - Madhloum, Narjes

AU - de Kok, Theo M.

AU - Nawrot, Tim S.

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AB - There is an increasing interest in microRNAs (miRNAs) as they are of utmost importance in gene regulation at the posttranscriptional level. Sex-related susceptibility for non-communicable diseases later in life could originate in early life. Until now, no data on sex-specific miRNA expression are available for the placenta. Therefore, we investigated the difference by sex of newborn's miRNA expression in human placental tissue. Within the ENVIRONAGE birth cohort, miRNA and mRNA expression profiling was performed in 60 placentae (50% boys) using Agilent (8 x 60 K) microarrays. The distribution of chromosome locations was studied and pathway analysis of the identified sex-specific miRNAs in the placenta was carried out. Of the total 2558 miRNAs on the array, 597 miRNAs were expressed in over 70% of the samples and were included for further analyses. A total of 142 miRNAs were significantly (FDR

KW - Placenta

KW - m(icro)RNA array

KW - sex-related differences

KW - MATERNAL ASTHMA

KW - FETAL SEX

KW - GENE-EXPRESSION

KW - METHYLATION

KW - GENDER

KW - BLOOD

KW - METABOLISM

KW - DIMORPHISM

KW - CARCINOMA

KW - HORMONE

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JO - Epigenetics

JF - Epigenetics

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ER -

Genome-wide microRNA expression analysis in human placenta reveals sex-specific patterns: an ENVIRONAGE birth cohort study

Abstract

Keywords

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