Genome Editing in Agriculture: Technical and Practical Considerations

Julia Jansing, Andreas Schiermeyer, Stefan Schillberg, Rainer Fischer, Luisa Bortesi*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

22 Citations (Web of Science)

Abstract

The advent of precise genome-editing tools has revolutionized the way we create new plant varieties. Three groups of tools are now available, classified according to their mechanism of action: Programmable sequence-specific nucleases, base-editing enzymes, and oligonucleotides. The corresponding techniques not only lead to different outcomes, but also have implications for the public acceptance and regulatory approval of genome-edited plants. Despite the high efficiency and precision of the tools, there are still major bottlenecks in the generation of new and improved varieties, including the efficient delivery of the genome-editing reagents, the selection of desired events, and the regeneration of intact plants. In this review, we evaluate current delivery and regeneration methods, discuss their suitability for important crop species, and consider the practical aspects of applying the different genome-editing techniques in agriculture.

Original languageEnglish
Article number2888
Number of pages33
JournalInternational journal of molecular sciences
Volume20
Issue number12
DOIs
Publication statusPublished - 2 Jun 2019

Keywords

  • base editors
  • oligonucleotide-directed mutagenesis
  • precision breeding
  • programmable nucleases
  • sequence-specific nucleases
  • AGROBACTERIUM-MEDIATED TRANSFORMATION
  • EFFICIENT TARGETED MUTAGENESIS
  • GENETICALLY-MODIFIED PLANTS
  • HOMOLOGOUS RECOMBINATION
  • SELECTABLE MARKER
  • DIRECTED MUTAGENESIS
  • ENGINEERED NUCLEASES
  • TETRAPLOID POTATO
  • CROP IMPROVEMENT
  • DNA REPLICONS

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