Genetic Markers of Brown Adipose Tissue Identity and In Vitro Brown Adipose Tissue Activity in Humans

Emmani B. M. Nascimento; Lauren M. Sparks; Adeline Divoux; Marike W. van Gisbergen; Evie P. M. Broeders; Johanna A. Jorgensen; Gert Schaart; Nicole D. Bouvy; Wouter D. van Marken Lichtenbelt; Patrick Schrauwen

doi:10.1002/oby.22062

Genetic Markers of Brown Adipose Tissue Identity and In Vitro Brown Adipose Tissue Activity in Humans

Emmani B. M. Nascimento^*, Lauren M. Sparks, Adeline Divoux, Marike W. van Gisbergen, Evie P. M. Broeders, Johanna A. Jorgensen, Gert Schaart, Nicole D. Bouvy, Wouter D. van Marken Lichtenbelt, Patrick Schrauwen

^*Corresponding author for this work

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Abstract

ObjectiveHuman brown adipose tissue (BAT) activity decreases with age and obesity. In addition to uncoupling protein 1 (UCP1), several genetic markers of BAT in humans have been published. However, the link between human BAT activity and genetic markers has been inadequately explored. MethodsWhite adipose tissue (WAT) and BAT biopsies were obtained from 16 patients undergoing deep neck surgery. In vitro differentiated adipocytes were used to measure norepinephrine-stimulated mitochondrial uncoupling as a measure of in vitro BAT activity. Gene expression was determined in adipose tissue biopsies. ResultsNorepinephrine increased in vitro BAT activity in adipocytes derived from human BAT, and this increase was abolished by propranolol. Furthermore, in vitro BAT activity showed a negative correlation to age and BMI. UCP1 messenger RNA (mRNA) expression showed a positive correlation to in vitro BAT activity, while zinc finger protein of cerebellum 1 (ZIC1) mRNA showed a negative correlation to in vitro BAT activity. In human BAT biopsies, UCP1 mRNA showed negative correlations to age and BMI, while ZIC1 mRNA showed positive correlations to age and BMI. ConclusionsDifferentiated adipocytes derived from human BAT maintain intrinsic characteristics of the donor. High ZIC1 mRNA does not necessarily reflect high BAT activity.

Original language	English
Pages (from-to)	135-140
Number of pages	6
Journal	Obesity
Volume	26
Issue number	1
DOIs	https://doi.org/10.1002/oby.22062
Publication status	Published - 1 Jan 2018

Keywords

ADULT HUMANS
FUNCTIONAL-CHARACTERIZATION
SKELETAL-MUSCLE
ADIPOCYTES
FAT
CELLS
IDENTIFICATION
DISTINCT
NECK

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10.1002/oby.22062

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Cite this

@article{019cf45050a64e649cce52b9a3a24946,

title = "Genetic Markers of Brown Adipose Tissue Identity and In Vitro Brown Adipose Tissue Activity in Humans",

abstract = "ObjectiveHuman brown adipose tissue (BAT) activity decreases with age and obesity. In addition to uncoupling protein 1 (UCP1), several genetic markers of BAT in humans have been published. However, the link between human BAT activity and genetic markers has been inadequately explored. MethodsWhite adipose tissue (WAT) and BAT biopsies were obtained from 16 patients undergoing deep neck surgery. In vitro differentiated adipocytes were used to measure norepinephrine-stimulated mitochondrial uncoupling as a measure of in vitro BAT activity. Gene expression was determined in adipose tissue biopsies. ResultsNorepinephrine increased in vitro BAT activity in adipocytes derived from human BAT, and this increase was abolished by propranolol. Furthermore, in vitro BAT activity showed a negative correlation to age and BMI. UCP1 messenger RNA (mRNA) expression showed a positive correlation to in vitro BAT activity, while zinc finger protein of cerebellum 1 (ZIC1) mRNA showed a negative correlation to in vitro BAT activity. In human BAT biopsies, UCP1 mRNA showed negative correlations to age and BMI, while ZIC1 mRNA showed positive correlations to age and BMI. ConclusionsDifferentiated adipocytes derived from human BAT maintain intrinsic characteristics of the donor. High ZIC1 mRNA does not necessarily reflect high BAT activity.",

keywords = "ADULT HUMANS, FUNCTIONAL-CHARACTERIZATION, SKELETAL-MUSCLE, ADIPOCYTES, FAT, CELLS, IDENTIFICATION, DISTINCT, NECK",

author = "Nascimento, {Emmani B. M.} and Sparks, {Lauren M.} and Adeline Divoux and {van Gisbergen}, {Marike W.} and Broeders, {Evie P. M.} and Jorgensen, {Johanna A.} and Gert Schaart and Bouvy, {Nicole D.} and {van Marken Lichtenbelt}, {Wouter D.} and Patrick Schrauwen",

year = "2018",

month = jan,

day = "1",

doi = "10.1002/oby.22062",

language = "English",

volume = "26",

pages = "135--140",

journal = "Obesity",

issn = "1930-7381",

publisher = "Wiley",

number = "1",

}

TY - JOUR

T1 - Genetic Markers of Brown Adipose Tissue Identity and In Vitro Brown Adipose Tissue Activity in Humans

AU - Nascimento, Emmani B. M.

AU - Sparks, Lauren M.

AU - Divoux, Adeline

AU - van Gisbergen, Marike W.

AU - Broeders, Evie P. M.

AU - Jorgensen, Johanna A.

AU - Schaart, Gert

AU - Bouvy, Nicole D.

AU - van Marken Lichtenbelt, Wouter D.

AU - Schrauwen, Patrick

PY - 2018/1/1

Y1 - 2018/1/1

N2 - ObjectiveHuman brown adipose tissue (BAT) activity decreases with age and obesity. In addition to uncoupling protein 1 (UCP1), several genetic markers of BAT in humans have been published. However, the link between human BAT activity and genetic markers has been inadequately explored. MethodsWhite adipose tissue (WAT) and BAT biopsies were obtained from 16 patients undergoing deep neck surgery. In vitro differentiated adipocytes were used to measure norepinephrine-stimulated mitochondrial uncoupling as a measure of in vitro BAT activity. Gene expression was determined in adipose tissue biopsies. ResultsNorepinephrine increased in vitro BAT activity in adipocytes derived from human BAT, and this increase was abolished by propranolol. Furthermore, in vitro BAT activity showed a negative correlation to age and BMI. UCP1 messenger RNA (mRNA) expression showed a positive correlation to in vitro BAT activity, while zinc finger protein of cerebellum 1 (ZIC1) mRNA showed a negative correlation to in vitro BAT activity. In human BAT biopsies, UCP1 mRNA showed negative correlations to age and BMI, while ZIC1 mRNA showed positive correlations to age and BMI. ConclusionsDifferentiated adipocytes derived from human BAT maintain intrinsic characteristics of the donor. High ZIC1 mRNA does not necessarily reflect high BAT activity.

AB - ObjectiveHuman brown adipose tissue (BAT) activity decreases with age and obesity. In addition to uncoupling protein 1 (UCP1), several genetic markers of BAT in humans have been published. However, the link between human BAT activity and genetic markers has been inadequately explored. MethodsWhite adipose tissue (WAT) and BAT biopsies were obtained from 16 patients undergoing deep neck surgery. In vitro differentiated adipocytes were used to measure norepinephrine-stimulated mitochondrial uncoupling as a measure of in vitro BAT activity. Gene expression was determined in adipose tissue biopsies. ResultsNorepinephrine increased in vitro BAT activity in adipocytes derived from human BAT, and this increase was abolished by propranolol. Furthermore, in vitro BAT activity showed a negative correlation to age and BMI. UCP1 messenger RNA (mRNA) expression showed a positive correlation to in vitro BAT activity, while zinc finger protein of cerebellum 1 (ZIC1) mRNA showed a negative correlation to in vitro BAT activity. In human BAT biopsies, UCP1 mRNA showed negative correlations to age and BMI, while ZIC1 mRNA showed positive correlations to age and BMI. ConclusionsDifferentiated adipocytes derived from human BAT maintain intrinsic characteristics of the donor. High ZIC1 mRNA does not necessarily reflect high BAT activity.

KW - ADULT HUMANS

KW - FUNCTIONAL-CHARACTERIZATION

KW - SKELETAL-MUSCLE

KW - ADIPOCYTES

KW - FAT

KW - CELLS

KW - IDENTIFICATION

KW - DISTINCT

KW - NECK

U2 - 10.1002/oby.22062

DO - 10.1002/oby.22062

M3 - Article

VL - 26

SP - 135

EP - 140

JO - Obesity

JF - Obesity

SN - 1930-7381

IS - 1

ER -