Generation of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9

Maria Rosaria Vitale; Johanna Eva Maria Zoeller; Charline Jansch; Anna Janz; Frank Edenhofer; Eva Klopocki; Daniel van den Hove; Tim Vanmierlo; Olga Rivero; Nael Nadif Kasri; Georg Christoph Ziegler; Klaus-Peter Lesch

doi:10.1016/j.scr.2021.102169

Generation of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9

Maria Rosaria Vitale^*
, Johanna Eva Maria Zoeller
, Charline Jansch
, Anna Janz
, Frank Edenhofer
, Eva Klopocki
, Daniel van den Hove
, Tim Vanmierlo
, Olga Rivero
, Nael Nadif Kasri
, Georg Christoph Ziegler
, Klaus-Peter Lesch^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Fibroblasts isolated from a skin biopsy of a healthy 46-year-old female were infected with Sendai virus containing the Yamanaka factors to produce transgene-free human induced pluripotent stem cells (iPSCs). CRISPR/Cas9 was used to generate isogenic cell lines with a gene dose-dependent deficiency of CDH13, a risk gene associated with neurodevelopmental and psychiatric disorders. Thereby, a heterozygous CDH13 knockout (CDH13(+/-)) and a CDH13 null mutant (CDH13(-/-)) iPSC line was obtained. All three lines showed expression of pluripotency-associated markers, the ability to differentiate into cells of the three germ layers in vitro, and a normal female karyotype.

Original language	English
Article number	102169
Pages (from-to)	1-5
Number of pages	5
Journal	Stem Cell Research
Volume	51
DOIs	https://doi.org/10.1016/j.scr.2021.102169
Publication status	Published - Mar 2021

Access to Document

10.1016/j.scr.2021.102169Licence: CC BY

Fingerprint

Dive into the research topics of 'Generation of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9'. Together they form a unique fingerprint.

Cite this

Vitale, M. R., Zoeller, J. E. M., Jansch, C., Janz, A., Edenhofer, F., Klopocki, E., van den Hove, D., Vanmierlo, T., Rivero, O., Kasri, N. N., Ziegler, G. C., & Lesch, K.-P. (2021). Generation of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9. Stem Cell Research, 51, 1-5. Article 102169. https://doi.org/10.1016/j.scr.2021.102169

@article{4b01598370eb4b5f9842dfd1a6c754df,

title = "Generation of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9",

abstract = "Fibroblasts isolated from a skin biopsy of a healthy 46-year-old female were infected with Sendai virus containing the Yamanaka factors to produce transgene-free human induced pluripotent stem cells (iPSCs). CRISPR/Cas9 was used to generate isogenic cell lines with a gene dose-dependent deficiency of CDH13, a risk gene associated with neurodevelopmental and psychiatric disorders. Thereby, a heterozygous CDH13 knockout (CDH13(+/-)) and a CDH13 null mutant (CDH13(-/-)) iPSC line was obtained. All three lines showed expression of pluripotency-associated markers, the ability to differentiate into cells of the three germ layers in vitro, and a normal female karyotype.",

author = "Vitale, \{Maria Rosaria\} and Zoeller, \{Johanna Eva Maria\} and Charline Jansch and Anna Janz and Frank Edenhofer and Eva Klopocki and \{van den Hove\}, Daniel and Tim Vanmierlo and Olga Rivero and Kasri, \{Nael Nadif\} and Ziegler, \{Georg Christoph\} and Klaus-Peter Lesch",

note = "Publisher Copyright: {\textcopyright} 2021 The Authors",

year = "2021",

month = mar,

doi = "10.1016/j.scr.2021.102169",

language = "English",

volume = "51",

pages = "1--5",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier B.V.",

}

Vitale, MR, Zoeller, JEM, Jansch, C, Janz, A, Edenhofer, F, Klopocki, E, van den Hove, D , Vanmierlo, T, Rivero, O, Kasri, NN, Ziegler, GC & Lesch, K-P 2021, 'Generation of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9', Stem Cell Research, vol. 51, 102169, pp. 1-5. https://doi.org/10.1016/j.scr.2021.102169

Generation of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9. / Vitale, Maria Rosaria; Zoeller, Johanna Eva Maria; Jansch, Charline et al.
In: Stem Cell Research, Vol. 51, 102169, 03.2021, p. 1-5.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Generation of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9

AU - Vitale, Maria Rosaria

AU - Zoeller, Johanna Eva Maria

AU - Jansch, Charline

AU - Janz, Anna

AU - Edenhofer, Frank

AU - Klopocki, Eva

AU - van den Hove, Daniel

AU - Vanmierlo, Tim

AU - Rivero, Olga

AU - Kasri, Nael Nadif

AU - Ziegler, Georg Christoph

AU - Lesch, Klaus-Peter

PY - 2021/3

Y1 - 2021/3

N2 - Fibroblasts isolated from a skin biopsy of a healthy 46-year-old female were infected with Sendai virus containing the Yamanaka factors to produce transgene-free human induced pluripotent stem cells (iPSCs). CRISPR/Cas9 was used to generate isogenic cell lines with a gene dose-dependent deficiency of CDH13, a risk gene associated with neurodevelopmental and psychiatric disorders. Thereby, a heterozygous CDH13 knockout (CDH13(+/-)) and a CDH13 null mutant (CDH13(-/-)) iPSC line was obtained. All three lines showed expression of pluripotency-associated markers, the ability to differentiate into cells of the three germ layers in vitro, and a normal female karyotype.

AB - Fibroblasts isolated from a skin biopsy of a healthy 46-year-old female were infected with Sendai virus containing the Yamanaka factors to produce transgene-free human induced pluripotent stem cells (iPSCs). CRISPR/Cas9 was used to generate isogenic cell lines with a gene dose-dependent deficiency of CDH13, a risk gene associated with neurodevelopmental and psychiatric disorders. Thereby, a heterozygous CDH13 knockout (CDH13(+/-)) and a CDH13 null mutant (CDH13(-/-)) iPSC line was obtained. All three lines showed expression of pluripotency-associated markers, the ability to differentiate into cells of the three germ layers in vitro, and a normal female karyotype.

U2 - 10.1016/j.scr.2021.102169

DO - 10.1016/j.scr.2021.102169

M3 - Article

C2 - 33486346

SN - 1873-5061

VL - 51

SP - 1

EP - 5

JO - Stem Cell Research

JF - Stem Cell Research

M1 - 102169

ER -