Generation of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9

Maria Rosaria Vitale; Johanna Eva Maria Zoeller; Charline Jansch; Anna Janz; Frank Edenhofer; Eva Klopocki; Daniel van den Hove; Tim Vanmierlo; Olga Rivero; Nael Nadif Kasri; Georg Christoph Ziegler; Klaus-Peter Lesch

doi:10.1016/j.scr.2021.102169

Generation of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9

Maria Rosaria Vitale^*, Johanna Eva Maria Zoeller, Charline Jansch, Anna Janz, Frank Edenhofer, Eva Klopocki, Daniel van den Hove, Tim Vanmierlo, Olga Rivero, Nael Nadif Kasri, Georg Christoph Ziegler, Klaus-Peter Lesch^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Fibroblasts isolated from a skin biopsy of a healthy 46-year-old female were infected with Sendai virus containing the Yamanaka factors to produce transgene-free human induced pluripotent stem cells (iPSCs). CRISPR/Cas9 was used to generate isogenic cell lines with a gene dose-dependent deficiency of CDH13, a risk gene associated with neurodevelopmental and psychiatric disorders. Thereby, a heterozygous CDH13 knockout (CDH13(+/-)) and a CDH13 null mutant (CDH13(-/-)) iPSC line was obtained. All three lines showed expression of pluripotency-associated markers, the ability to differentiate into cells of the three germ layers in vitro, and a normal female karyotype.

Original language	English
Article number	102169
Pages (from-to)	1-5
Number of pages	5
Journal	Stem Cell Research
Volume	51
DOIs	https://doi.org/10.1016/j.scr.2021.102169
Publication status	Published - Mar 2021

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Vitale, M. R., Zoeller, J. E. M., Jansch, C., Janz, A., Edenhofer, F., Klopocki, E., van den Hove, D., Vanmierlo, T., Rivero, O., Kasri, N. N., Ziegler, G. C., & Lesch, K.-P. (2021). Generation of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9. Stem Cell Research, 51, 1-5. Article 102169. https://doi.org/10.1016/j.scr.2021.102169

@article{4b01598370eb4b5f9842dfd1a6c754df,

title = "Generation of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9",

abstract = "Fibroblasts isolated from a skin biopsy of a healthy 46-year-old female were infected with Sendai virus containing the Yamanaka factors to produce transgene-free human induced pluripotent stem cells (iPSCs). CRISPR/Cas9 was used to generate isogenic cell lines with a gene dose-dependent deficiency of CDH13, a risk gene associated with neurodevelopmental and psychiatric disorders. Thereby, a heterozygous CDH13 knockout (CDH13(+/-)) and a CDH13 null mutant (CDH13(-/-)) iPSC line was obtained. All three lines showed expression of pluripotency-associated markers, the ability to differentiate into cells of the three germ layers in vitro, and a normal female karyotype.",

author = "Vitale, {Maria Rosaria} and Zoeller, {Johanna Eva Maria} and Charline Jansch and Anna Janz and Frank Edenhofer and Eva Klopocki and {van den Hove}, Daniel and Tim Vanmierlo and Olga Rivero and Kasri, {Nael Nadif} and Ziegler, {Georg Christoph} and Klaus-Peter Lesch",

note = "Publisher Copyright: {\textcopyright} 2021 The Authors",

year = "2021",

month = mar,

doi = "10.1016/j.scr.2021.102169",

language = "English",

volume = "51",

pages = "1--5",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier",

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Vitale, MR, Zoeller, JEM, Jansch, C, Janz, A, Edenhofer, F, Klopocki, E, van den Hove, D , Vanmierlo, T, Rivero, O, Kasri, NN, Ziegler, GC & Lesch, K-P 2021, 'Generation of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9', Stem Cell Research, vol. 51, 102169, pp. 1-5. https://doi.org/10.1016/j.scr.2021.102169

Generation of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9. / Vitale, Maria Rosaria; Zoeller, Johanna Eva Maria; Jansch, Charline et al.
In: Stem Cell Research, Vol. 51, 102169, 03.2021, p. 1-5.

Research output: Contribution to journal › Article › Academic › peer-review

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AU - Zoeller, Johanna Eva Maria

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AU - van den Hove, Daniel

AU - Vanmierlo, Tim

AU - Rivero, Olga

AU - Kasri, Nael Nadif

AU - Ziegler, Georg Christoph

AU - Lesch, Klaus-Peter

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AB - Fibroblasts isolated from a skin biopsy of a healthy 46-year-old female were infected with Sendai virus containing the Yamanaka factors to produce transgene-free human induced pluripotent stem cells (iPSCs). CRISPR/Cas9 was used to generate isogenic cell lines with a gene dose-dependent deficiency of CDH13, a risk gene associated with neurodevelopmental and psychiatric disorders. Thereby, a heterozygous CDH13 knockout (CDH13(+/-)) and a CDH13 null mutant (CDH13(-/-)) iPSC line was obtained. All three lines showed expression of pluripotency-associated markers, the ability to differentiate into cells of the three germ layers in vitro, and a normal female karyotype.

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