Generation of a ST3GAL3 null mutant induced pluripotent stem cell (iPSC) line (UKWMPi002-A-3) by CRISPR/Cas9 genome editing

D. Diouf*, M.R. Vitale, J.E.M. Zoller, A.M. Pineau, E. Klopocki, C. Hamann, G.C. Ziegler, T. Vanmierlo, D. Van den Hove, K.P. Lesch*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Fibroblasts isolated from a skin biopsy of a healthy individual were infected with Sendai virus containing the Yamanaka factors to produce transgene-free human induced pluripotent stem cells (iPSCs). CRISPR/Cas9 was used to generate an isogenic cell line carrying an inactivation of ST3GAL3, a risk gene associated with neurodevelopmental and psychiatric disorders. This ST3GAL3 null mutant (ST3GAL3-/-) iPSC line, which displays the expression of pluripotency-associated markers, the ability to differentiate into cells of the three germ layers in vitro, and a normal karyotype, is a powerful tool to investigate the impact of deficient sialylation of glycoproteins in neural development and plasticity.
Original languageEnglish
Article number103038
Number of pages6
JournalStem Cell Research
Volume67
Issue number1
DOIs
Publication statusPublished - 1 Mar 2023

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