Generation of a ST3GAL3 null mutant induced pluripotent stem cell (iPSC) line (UKWMPi002-A-3) by CRISPR/Cas9 genome editing

D. Diouf*, M.R. Vitale, J.E.M. Zoller, A.M. Pineau, E. Klopocki, C. Hamann, G.C. Ziegler, T. Vanmierlo, D. Van den Hove, K.P. Lesch*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


Fibroblasts isolated from a skin biopsy of a healthy individual were infected with Sendai virus containing the Yamanaka factors to produce transgene-free human induced pluripotent stem cells (iPSCs). CRISPR/Cas9 was used to generate an isogenic cell line carrying an inactivation of ST3GAL3, a risk gene associated with neurodevelopmental and psychiatric disorders. This ST3GAL3 null mutant (ST3GAL3-/-) iPSC line, which displays the expression of pluripotency-associated markers, the ability to differentiate into cells of the three germ layers in vitro, and a normal karyotype, is a powerful tool to investigate the impact of deficient sialylation of glycoproteins in neural development and plasticity.
Original languageEnglish
Article number103038
Number of pages6
JournalStem Cell Research
Publication statusPublished - 1 Mar 2023

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