Generation of a ST3GAL3 null mutant induced pluripotent stem cell (iPSC) line (UKWMPi002-A-3) by CRISPR/Cas9 genome editing

D. Diouf; M.R. Vitale; J.E.M. Zoller; A.M. Pineau; E. Klopocki; C. Hamann; G.C. Ziegler; T. Vanmierlo; D. Van den Hove; K.P. Lesch

doi:10.1016/j.scr.2023.103038

Generation of a ST3GAL3 null mutant induced pluripotent stem cell (iPSC) line (UKWMPi002-A-3) by CRISPR/Cas9 genome editing

D. Diouf^*, M.R. Vitale, J.E.M. Zoller, A.M. Pineau, E. Klopocki, C. Hamann, G.C. Ziegler, T. Vanmierlo, D. Van den Hove, K.P. Lesch^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Fibroblasts isolated from a skin biopsy of a healthy individual were infected with Sendai virus containing the Yamanaka factors to produce transgene-free human induced pluripotent stem cells (iPSCs). CRISPR/Cas9 was used to generate an isogenic cell line carrying an inactivation of ST3GAL3, a risk gene associated with neurodevelopmental and psychiatric disorders. This ST3GAL3 null mutant (ST3GAL3-/-) iPSC line, which displays the expression of pluripotency-associated markers, the ability to differentiate into cells of the three germ layers in vitro, and a normal karyotype, is a powerful tool to investigate the impact of deficient sialylation of glycoproteins in neural development and plasticity.

Original language	English
Article number	103038
Number of pages	6
Journal	Stem Cell Research
Volume	67
DOIs	https://doi.org/10.1016/j.scr.2023.103038
Publication status	Published - 1 Mar 2023

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10.1016/j.scr.2023.103038Licence: CC BY

Cite this

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title = "Generation of a ST3GAL3 null mutant induced pluripotent stem cell (iPSC) line (UKWMPi002-A-3) by CRISPR/Cas9 genome editing",

abstract = "Fibroblasts isolated from a skin biopsy of a healthy individual were infected with Sendai virus containing the Yamanaka factors to produce transgene-free human induced pluripotent stem cells (iPSCs). CRISPR/Cas9 was used to generate an isogenic cell line carrying an inactivation of ST3GAL3, a risk gene associated with neurodevelopmental and psychiatric disorders. This ST3GAL3 null mutant (ST3GAL3-/-) iPSC line, which displays the expression of pluripotency-associated markers, the ability to differentiate into cells of the three germ layers in vitro, and a normal karyotype, is a powerful tool to investigate the impact of deficient sialylation of glycoproteins in neural development and plasticity.",

author = "D. Diouf and M.R. Vitale and J.E.M. Zoller and A.M. Pineau and E. Klopocki and C. Hamann and G.C. Ziegler and T. Vanmierlo and {Van den Hove}, D. and K.P. Lesch",

year = "2023",

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doi = "10.1016/j.scr.2023.103038",

language = "English",

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T1 - Generation of a ST3GAL3 null mutant induced pluripotent stem cell (iPSC) line (UKWMPi002-A-3) by CRISPR/Cas9 genome editing

AU - Diouf, D.

AU - Vitale, M.R.

AU - Zoller, J.E.M.

AU - Pineau, A.M.

AU - Klopocki, E.

AU - Hamann, C.

AU - Ziegler, G.C.

AU - Vanmierlo, T.

AU - Van den Hove, D.

AU - Lesch, K.P.

PY - 2023/3/1

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AB - Fibroblasts isolated from a skin biopsy of a healthy individual were infected with Sendai virus containing the Yamanaka factors to produce transgene-free human induced pluripotent stem cells (iPSCs). CRISPR/Cas9 was used to generate an isogenic cell line carrying an inactivation of ST3GAL3, a risk gene associated with neurodevelopmental and psychiatric disorders. This ST3GAL3 null mutant (ST3GAL3-/-) iPSC line, which displays the expression of pluripotency-associated markers, the ability to differentiate into cells of the three germ layers in vitro, and a normal karyotype, is a powerful tool to investigate the impact of deficient sialylation of glycoproteins in neural development and plasticity.

U2 - 10.1016/j.scr.2023.103038

DO - 10.1016/j.scr.2023.103038

M3 - Article

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