Generation of a human induced pluripotent stem cell (iPSC) line from a 51-year-old female with attention-deficit/hyperactivity disorder (ADHD) carrying a duplication of SLC2A3

Charline Jansch; Katharina Gunther; Jonas Waider; Georg C. Ziegler; Andrea Forero; Sina Kollert; Evgeniy Svirin; Dirk Puhringer; Chee Keong Kwok; Reinhard Ullmann; Anna Maierhofer; Julia Flunkert; Thomas Haaf; Frank Edenhofer; Klaus-Peter Lesch

doi:10.1016/j.scr.2018.02.005

Generation of a human induced pluripotent stem cell (iPSC) line from a 51-year-old female with attention-deficit/hyperactivity disorder (ADHD) carrying a duplication of SLC2A3

Charline Jansch
, Katharina Gunther
, Jonas Waider
, Georg C. Ziegler
, Andrea Forero
, Sina Kollert
, Evgeniy Svirin
, Dirk Puhringer
, Chee Keong Kwok
, Reinhard Ullmann
, Anna Maierhofer
, Julia Flunkert
, Thomas Haaf
, Frank Edenhofer^*
, Klaus-Peter Lesch^*

^*Corresponding author for this work

Research output: Contribution to journal › Editorial › Academic › peer-review

Abstract

Fibroblasts were isolated from a skin biopsy of a clinically diagnosed 51-year-old female attention-deficit/hyperactivity disorder (ADHD) patient carrying a duplication of SLC2A3, a gene encoding neuronal glucose transporter-3 (GLUT3). Patient fibroblasts were infected with Sendai virus, a single-stranded RNA virus, to generate transgene-free human induced pluripotent stem cells (iPSCs). SLC2A3-D2-iPSCs showed expression of pluripotency-associated markers, were able to differentiate into cells of the three germ layers in vitro and had a normal female karyotype. This in vitro cellular model can be used to study the role of risk genes in the pathogenesis of ADHD, in a patient-specific manner. (c) 2018 Published by Elsevier B.V.

Original language	English
Pages (from-to)	136-140
Number of pages	5
Journal	Stem Cell Research
Volume	28
DOIs	https://doi.org/10.1016/j.scr.2018.02.005
Publication status	Published - 1 Apr 2018

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https://doi.org/10.1016/j.scr.2018.02.005

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Jansch, C., Gunther, K., Waider, J., Ziegler, G. C., Forero, A., Kollert, S., Svirin, E., Puhringer, D., Kwok, C. K., Ullmann, R., Maierhofer, A., Flunkert, J., Haaf, T., Edenhofer, F., & Lesch, K.-P. (2018). Generation of a human induced pluripotent stem cell (iPSC) line from a 51-year-old female with attention-deficit/hyperactivity disorder (ADHD) carrying a duplication of SLC2A3. Stem Cell Research, 28, 136-140. https://doi.org/10.1016/j.scr.2018.02.005

@article{ebeb381607404f8b8c05dff09197987b,

title = "Generation of a human induced pluripotent stem cell (iPSC) line from a 51-year-old female with attention-deficit/hyperactivity disorder (ADHD) carrying a duplication of SLC2A3",

abstract = "Fibroblasts were isolated from a skin biopsy of a clinically diagnosed 51-year-old female attention-deficit/hyperactivity disorder (ADHD) patient carrying a duplication of SLC2A3, a gene encoding neuronal glucose transporter-3 (GLUT3). Patient fibroblasts were infected with Sendai virus, a single-stranded RNA virus, to generate transgene-free human induced pluripotent stem cells (iPSCs). SLC2A3-D2-iPSCs showed expression of pluripotency-associated markers, were able to differentiate into cells of the three germ layers in vitro and had a normal female karyotype. This in vitro cellular model can be used to study the role of risk genes in the pathogenesis of ADHD, in a patient-specific manner. (c) 2018 Published by Elsevier B.V.",

author = "Charline Jansch and Katharina Gunther and Jonas Waider and Ziegler, \{Georg C.\} and Andrea Forero and Sina Kollert and Evgeniy Svirin and Dirk Puhringer and Kwok, \{Chee Keong\} and Reinhard Ullmann and Anna Maierhofer and Julia Flunkert and Thomas Haaf and Frank Edenhofer and Klaus-Peter Lesch",

year = "2018",

month = apr,

day = "1",

doi = "10.1016/j.scr.2018.02.005",

language = "English",

volume = "28",

pages = "136--140",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier B.V.",

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Jansch, C, Gunther, K, Waider, J, Ziegler, GC, Forero, A, Kollert, S, Svirin, E, Puhringer, D, Kwok, CK, Ullmann, R, Maierhofer, A, Flunkert, J, Haaf, T, Edenhofer, F & Lesch, K-P 2018, 'Generation of a human induced pluripotent stem cell (iPSC) line from a 51-year-old female with attention-deficit/hyperactivity disorder (ADHD) carrying a duplication of SLC2A3', Stem Cell Research, vol. 28, pp. 136-140. https://doi.org/10.1016/j.scr.2018.02.005

Generation of a human induced pluripotent stem cell (iPSC) line from a 51-year-old female with attention-deficit/hyperactivity disorder (ADHD) carrying a duplication of SLC2A3. / Jansch, Charline; Gunther, Katharina; Waider, Jonas et al.
In: Stem Cell Research, Vol. 28, 01.04.2018, p. 136-140.

Research output: Contribution to journal › Editorial › Academic › peer-review

TY - JOUR

T1 - Generation of a human induced pluripotent stem cell (iPSC) line from a 51-year-old female with attention-deficit/hyperactivity disorder (ADHD) carrying a duplication of SLC2A3

AU - Jansch, Charline

AU - Gunther, Katharina

AU - Waider, Jonas

AU - Ziegler, Georg C.

AU - Forero, Andrea

AU - Kollert, Sina

AU - Svirin, Evgeniy

AU - Puhringer, Dirk

AU - Kwok, Chee Keong

AU - Ullmann, Reinhard

AU - Maierhofer, Anna

AU - Flunkert, Julia

AU - Haaf, Thomas

AU - Edenhofer, Frank

AU - Lesch, Klaus-Peter

PY - 2018/4/1

Y1 - 2018/4/1

N2 - Fibroblasts were isolated from a skin biopsy of a clinically diagnosed 51-year-old female attention-deficit/hyperactivity disorder (ADHD) patient carrying a duplication of SLC2A3, a gene encoding neuronal glucose transporter-3 (GLUT3). Patient fibroblasts were infected with Sendai virus, a single-stranded RNA virus, to generate transgene-free human induced pluripotent stem cells (iPSCs). SLC2A3-D2-iPSCs showed expression of pluripotency-associated markers, were able to differentiate into cells of the three germ layers in vitro and had a normal female karyotype. This in vitro cellular model can be used to study the role of risk genes in the pathogenesis of ADHD, in a patient-specific manner. (c) 2018 Published by Elsevier B.V.

AB - Fibroblasts were isolated from a skin biopsy of a clinically diagnosed 51-year-old female attention-deficit/hyperactivity disorder (ADHD) patient carrying a duplication of SLC2A3, a gene encoding neuronal glucose transporter-3 (GLUT3). Patient fibroblasts were infected with Sendai virus, a single-stranded RNA virus, to generate transgene-free human induced pluripotent stem cells (iPSCs). SLC2A3-D2-iPSCs showed expression of pluripotency-associated markers, were able to differentiate into cells of the three germ layers in vitro and had a normal female karyotype. This in vitro cellular model can be used to study the role of risk genes in the pathogenesis of ADHD, in a patient-specific manner. (c) 2018 Published by Elsevier B.V.

U2 - 10.1016/j.scr.2018.02.005

DO - 10.1016/j.scr.2018.02.005

M3 - Editorial

C2 - 29477591

SN - 1873-5061

VL - 28

SP - 136

EP - 140

JO - Stem Cell Research

JF - Stem Cell Research

ER -

Generation of a human induced pluripotent stem cell (iPSC) line from a 51-year-old female with attention-deficit/hyperactivity disorder (ADHD) carrying a duplication of SLC2A3

Abstract

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