Generation of a human induced pluripotent stem cell (iPSC) line from a 51-year-old female with attention-deficit/hyperactivity disorder (ADHD) carrying a duplication of SLC2A3

Charline Jansch; Katharina Gunther; Jonas Waider; Georg C. Ziegler; Andrea Forero; Sina Kollert; Evgeniy Svirin; Dirk Puhringer; Chee Keong Kwok; Reinhard Ullmann; Anna Maierhofer; Julia Flunkert; Thomas Haaf; Frank Edenhofer; Klaus-Peter Lesch

doi:10.1016/j.scr.2018.02.005

Generation of a human induced pluripotent stem cell (iPSC) line from a 51-year-old female with attention-deficit/hyperactivity disorder (ADHD) carrying a duplication of SLC2A3

Charline Jansch, Katharina Gunther, Jonas Waider, Georg C. Ziegler, Andrea Forero, Sina Kollert, Evgeniy Svirin, Dirk Puhringer, Chee Keong Kwok, Reinhard Ullmann, Anna Maierhofer, Julia Flunkert, Thomas Haaf, Frank Edenhofer^*, Klaus-Peter Lesch^*

^*Corresponding author for this work

Research output: Contribution to journal › Editorial › Academic › peer-review

Abstract

Fibroblasts were isolated from a skin biopsy of a clinically diagnosed 51-year-old female attention-deficit/hyperactivity disorder (ADHD) patient carrying a duplication of SLC2A3, a gene encoding neuronal glucose transporter-3 (GLUT3). Patient fibroblasts were infected with Sendai virus, a single-stranded RNA virus, to generate transgene-free human induced pluripotent stem cells (iPSCs). SLC2A3-D2-iPSCs showed expression of pluripotency-associated markers, were able to differentiate into cells of the three germ layers in vitro and had a normal female karyotype. This in vitro cellular model can be used to study the role of risk genes in the pathogenesis of ADHD, in a patient-specific manner. (c) 2018 Published by Elsevier B.V.

Original language	English
Pages (from-to)	136-140
Number of pages	5
Journal	Stem Cell Research
Volume	28
DOIs	https://doi.org/10.1016/j.scr.2018.02.005
Publication status	Published - 1 Apr 2018

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Jansch, C., Gunther, K., Waider, J., Ziegler, G. C., Forero, A., Kollert, S., Svirin, E., Puhringer, D., Kwok, C. K., Ullmann, R., Maierhofer, A., Flunkert, J., Haaf, T., Edenhofer, F., & Lesch, K.-P. (2018). Generation of a human induced pluripotent stem cell (iPSC) line from a 51-year-old female with attention-deficit/hyperactivity disorder (ADHD) carrying a duplication of SLC2A3. Stem Cell Research, 28, 136-140. https://doi.org/10.1016/j.scr.2018.02.005

@article{ebeb381607404f8b8c05dff09197987b,

title = "Generation of a human induced pluripotent stem cell (iPSC) line from a 51-year-old female with attention-deficit/hyperactivity disorder (ADHD) carrying a duplication of SLC2A3",

abstract = "Fibroblasts were isolated from a skin biopsy of a clinically diagnosed 51-year-old female attention-deficit/hyperactivity disorder (ADHD) patient carrying a duplication of SLC2A3, a gene encoding neuronal glucose transporter-3 (GLUT3). Patient fibroblasts were infected with Sendai virus, a single-stranded RNA virus, to generate transgene-free human induced pluripotent stem cells (iPSCs). SLC2A3-D2-iPSCs showed expression of pluripotency-associated markers, were able to differentiate into cells of the three germ layers in vitro and had a normal female karyotype. This in vitro cellular model can be used to study the role of risk genes in the pathogenesis of ADHD, in a patient-specific manner. (c) 2018 Published by Elsevier B.V.",

author = "Charline Jansch and Katharina Gunther and Jonas Waider and Ziegler, {Georg C.} and Andrea Forero and Sina Kollert and Evgeniy Svirin and Dirk Puhringer and Kwok, {Chee Keong} and Reinhard Ullmann and Anna Maierhofer and Julia Flunkert and Thomas Haaf and Frank Edenhofer and Klaus-Peter Lesch",

year = "2018",

month = apr,

day = "1",

doi = "10.1016/j.scr.2018.02.005",

language = "English",

volume = "28",

pages = "136--140",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier",

}

Jansch, C, Gunther, K, Waider, J, Ziegler, GC, Forero, A, Kollert, S, Svirin, E, Puhringer, D, Kwok, CK, Ullmann, R, Maierhofer, A, Flunkert, J, Haaf, T, Edenhofer, F & Lesch, K-P 2018, 'Generation of a human induced pluripotent stem cell (iPSC) line from a 51-year-old female with attention-deficit/hyperactivity disorder (ADHD) carrying a duplication of SLC2A3', Stem Cell Research, vol. 28, pp. 136-140. https://doi.org/10.1016/j.scr.2018.02.005

Generation of a human induced pluripotent stem cell (iPSC) line from a 51-year-old female with attention-deficit/hyperactivity disorder (ADHD) carrying a duplication of SLC2A3. / Jansch, Charline; Gunther, Katharina; Waider, Jonas et al.
In: Stem Cell Research, Vol. 28, 01.04.2018, p. 136-140.

Research output: Contribution to journal › Editorial › Academic › peer-review

TY - JOUR

T1 - Generation of a human induced pluripotent stem cell (iPSC) line from a 51-year-old female with attention-deficit/hyperactivity disorder (ADHD) carrying a duplication of SLC2A3

AU - Jansch, Charline

AU - Gunther, Katharina

AU - Waider, Jonas

AU - Ziegler, Georg C.

AU - Forero, Andrea

AU - Kollert, Sina

AU - Svirin, Evgeniy

AU - Puhringer, Dirk

AU - Kwok, Chee Keong

AU - Ullmann, Reinhard

AU - Maierhofer, Anna

AU - Flunkert, Julia

AU - Haaf, Thomas

AU - Edenhofer, Frank

AU - Lesch, Klaus-Peter

PY - 2018/4/1

Y1 - 2018/4/1

N2 - Fibroblasts were isolated from a skin biopsy of a clinically diagnosed 51-year-old female attention-deficit/hyperactivity disorder (ADHD) patient carrying a duplication of SLC2A3, a gene encoding neuronal glucose transporter-3 (GLUT3). Patient fibroblasts were infected with Sendai virus, a single-stranded RNA virus, to generate transgene-free human induced pluripotent stem cells (iPSCs). SLC2A3-D2-iPSCs showed expression of pluripotency-associated markers, were able to differentiate into cells of the three germ layers in vitro and had a normal female karyotype. This in vitro cellular model can be used to study the role of risk genes in the pathogenesis of ADHD, in a patient-specific manner. (c) 2018 Published by Elsevier B.V.

AB - Fibroblasts were isolated from a skin biopsy of a clinically diagnosed 51-year-old female attention-deficit/hyperactivity disorder (ADHD) patient carrying a duplication of SLC2A3, a gene encoding neuronal glucose transporter-3 (GLUT3). Patient fibroblasts were infected with Sendai virus, a single-stranded RNA virus, to generate transgene-free human induced pluripotent stem cells (iPSCs). SLC2A3-D2-iPSCs showed expression of pluripotency-associated markers, were able to differentiate into cells of the three germ layers in vitro and had a normal female karyotype. This in vitro cellular model can be used to study the role of risk genes in the pathogenesis of ADHD, in a patient-specific manner. (c) 2018 Published by Elsevier B.V.

U2 - 10.1016/j.scr.2018.02.005

DO - 10.1016/j.scr.2018.02.005

M3 - Editorial

C2 - 29477591

SN - 1873-5061

VL - 28

SP - 136

EP - 140

JO - Stem Cell Research

JF - Stem Cell Research

ER -

Generation of a human induced pluripotent stem cell (iPSC) line from a 51-year-old female with attention-deficit/hyperactivity disorder (ADHD) carrying a duplication of SLC2A3

Abstract

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