Fundamentals of light-cell-polymer interactions in photo-cross-linking based bioprinting

Daniel Nieto*, Juan Antonio Marchal Corrales, Alberto Jorge de Mora, Lorenzo Moroni

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

16 Citations (Web of Science)
48 Downloads (Pure)

Abstract

Biofabrication technologies that use light for polymerization of biomaterials have made significant progress in the quality, resolution, and generation of precise complex tissue structures. In recent years, the evolution of these technologies has been growing along with the development of new photocurable resins and photoinitiators that are biocompatible and biodegradable with bioactive properties. Such evolution has allowed the progress of a large number of tissue engineering applications. Flexibility in the design, scale, and resolution and wide applicability of technologies are strongly dependent on the understanding of the biophysics involved in the biofabrication process. In particular, understanding cell-light interactions is crucial when bioprinting using cell-laden biomaterials. Here, we summarize some theoretical mechanisms, which condition cell response during bioprinting using light based technologies. We take a brief look at the light-biomaterial interaction for a better understanding of how linear effects (refraction, reflection, absorption, emission, and scattering) and nonlinear effects (two-photon absorption) influence the biofabricated tissue structures and identify the different parameters essential for maintaining cell viability during and after bioprinting. (C) 2020 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

Original languageEnglish
Article number041502
Number of pages14
JournalAPL Bioengineering
Volume4
Issue number4
DOIs
Publication statusPublished - 1 Dec 2020

Keywords

  • INDUCED DNA-DAMAGE
  • 2-PHOTON POLYMERIZATION
  • VISIBLE-LIGHT
  • 3-DIMENSIONAL MICROFABRICATION
  • STEM-CELLS
  • TISSUE
  • STEREOLITHOGRAPHY
  • HYDROGELS
  • FABRICATION
  • PHOTOPOLYMERIZATION

Cite this