Functionalized Collagen I Membranes as a Bruch's Membrane Mimetic for Outer Retinal In Vitro Models

Ashley R. Murphy, Xuen Jen Ng, Grace Lidgerwood, Alice Pebay, Yen B. Truong, Carmel M. O'Brien, Veronica Glattauer*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Physiologically relevant in vitro models of the human outer retina are required to better elucidate the complex interplay of retinal tissue layers and investigate their role in retinal degenerative disorders. Materials currently used to mimic the function of Bruch's membrane fail to replicate a range of important structural, mechanical, and biochemical properties. Here, we detail the fabrication of a surface-functionalized, fibrous collagen I membrane. We demonstrate its ability to better replicate a range of important material properties akin to the function of human Bruch's membrane when compared with a commonly utilized synthetic polyethylene terephthalate alternative. We further reveal the ability of this membrane to support the culture of the ARPE-19 cell line, as well as human pluripotent stem cell-derived RPE-like cells and human umbilical vein endothelial cells. This material could provide greater physiological relevance to the native Bruch's membrane than current synthetic materials and further improve the outcomes of in vitro outer retinal models.
Original languageEnglish
Pages (from-to)5653-5665
Number of pages13
JournalACS Biomaterial Science and Engineering
Volume10
Issue number9
DOIs
Publication statusPublished - 9 Sept 2024
Externally publishedYes

Keywords

  • Bruch's membrane
  • retina
  • retinal pigmentepithelium
  • collagen
  • in vitro model
  • PIGMENT EPITHELIUM
  • MACROMOLECULAR DIFFUSION
  • AGE
  • CELLS
  • RPE

Fingerprint

Dive into the research topics of 'Functionalized Collagen I Membranes as a Bruch's Membrane Mimetic for Outer Retinal In Vitro Models'. Together they form a unique fingerprint.

Cite this