Functional mucous layer and healing of proximal colonic anastomoses in an experimental model

J. W. A. M. Bosmans; A. C. H. M. Jongen; G. M. H. Birchenough; E. E. L. Nystrom; M. J. J. Gijbels; J. P. M. Derikx; N. D. Bouvy; G. C. Hansson

doi:10.1002/bjs.10456

Functional mucous layer and healing of proximal colonic anastomoses in an experimental model

J. W. A. M. Bosmans^*, A. C. H. M. Jongen, G. M. H. Birchenough, E. E. L. Nystrom, M. J. J. Gijbels, J. P. M. Derikx, N. D. Bouvy, G. C. Hansson

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

BackgroundAnastomotic leakage (AL) is the most dreaded complication after colorectal surgery, causing high morbidity and mortality. Mucus is a first line of defence against external factors in the gastrointestinal tract. In this study, the structural mucus protein Muc2 was depleted in genetically engineered mice and the effect on healing of colonic anastomoses studied in an experimental model.

MethodsMice of different Muc2 genotypes were used in a proximal colonic AL model. Tissues were scored histologically for inflammation, bacterial translocation was determined by quantitative PCR of bacterial 16S ribosomal DNA, and epithelial cell damage was determined by assessing serum levels of intestinal fatty acid-binding protein.

ResultsOf 22 Muc2-deficient (Muc2(-/-)) mice, 20 developed AL, compared with seven of 22 control animals (P

ConclusionA functional mucous layer facilitates the healing of colonic anastomoses.

Mucus matters

Original language	English
Pages (from-to)	619-630
Number of pages	12
Journal	British Journal of Surgery
Volume	104
Issue number	5
DOIs	https://doi.org/10.1002/bjs.10456
Publication status	Published - Apr 2017

Keywords

RISK-FACTORS
GOBLET CELL
GASTROINTESTINAL-TRACT
ANTERIOR RESECTION
COLORECTAL-CANCER
INTESTINAL DAMAGE
MUCUS LAYERS
RAT COLON
LEAKAGE
SURGERY

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10.1002/bjs.10456

Cite this

@article{1690fe7ee62e46eeb11c734bb53680f6,

title = "Functional mucous layer and healing of proximal colonic anastomoses in an experimental model",

abstract = "BackgroundAnastomotic leakage (AL) is the most dreaded complication after colorectal surgery, causing high morbidity and mortality. Mucus is a first line of defence against external factors in the gastrointestinal tract. In this study, the structural mucus protein Muc2 was depleted in genetically engineered mice and the effect on healing of colonic anastomoses studied in an experimental model.MethodsMice of different Muc2 genotypes were used in a proximal colonic AL model. Tissues were scored histologically for inflammation, bacterial translocation was determined by quantitative PCR of bacterial 16S ribosomal DNA, and epithelial cell damage was determined by assessing serum levels of intestinal fatty acid-binding protein.ResultsOf 22 Muc2-deficient (Muc2(-/-)) mice, 20 developed AL, compared with seven of 22 control animals (PConclusionA functional mucous layer facilitates the healing of colonic anastomoses.Mucus matters",

keywords = "RISK-FACTORS, GOBLET CELL, GASTROINTESTINAL-TRACT, ANTERIOR RESECTION, COLORECTAL-CANCER, INTESTINAL DAMAGE, MUCUS LAYERS, RAT COLON, LEAKAGE, SURGERY",

author = "Bosmans, {J. W. A. M.} and Jongen, {A. C. H. M.} and Birchenough, {G. M. H.} and Nystrom, {E. E. L.} and Gijbels, {M. J. J.} and Derikx, {J. P. M.} and Bouvy, {N. D.} and Hansson, {G. C.}",

year = "2017",

month = apr,

doi = "10.1002/bjs.10456",

language = "English",

volume = "104",

pages = "619--630",

journal = "British Journal of Surgery",

issn = "0007-1323",

publisher = "Wiley",

number = "5",

}

TY - JOUR

T1 - Functional mucous layer and healing of proximal colonic anastomoses in an experimental model

AU - Bosmans, J. W. A. M.

AU - Jongen, A. C. H. M.

AU - Birchenough, G. M. H.

AU - Nystrom, E. E. L.

AU - Gijbels, M. J. J.

AU - Derikx, J. P. M.

AU - Bouvy, N. D.

AU - Hansson, G. C.

PY - 2017/4

Y1 - 2017/4

N2 - BackgroundAnastomotic leakage (AL) is the most dreaded complication after colorectal surgery, causing high morbidity and mortality. Mucus is a first line of defence against external factors in the gastrointestinal tract. In this study, the structural mucus protein Muc2 was depleted in genetically engineered mice and the effect on healing of colonic anastomoses studied in an experimental model.MethodsMice of different Muc2 genotypes were used in a proximal colonic AL model. Tissues were scored histologically for inflammation, bacterial translocation was determined by quantitative PCR of bacterial 16S ribosomal DNA, and epithelial cell damage was determined by assessing serum levels of intestinal fatty acid-binding protein.ResultsOf 22 Muc2-deficient (Muc2(-/-)) mice, 20 developed AL, compared with seven of 22 control animals (PConclusionA functional mucous layer facilitates the healing of colonic anastomoses.Mucus matters

AB - BackgroundAnastomotic leakage (AL) is the most dreaded complication after colorectal surgery, causing high morbidity and mortality. Mucus is a first line of defence against external factors in the gastrointestinal tract. In this study, the structural mucus protein Muc2 was depleted in genetically engineered mice and the effect on healing of colonic anastomoses studied in an experimental model.MethodsMice of different Muc2 genotypes were used in a proximal colonic AL model. Tissues were scored histologically for inflammation, bacterial translocation was determined by quantitative PCR of bacterial 16S ribosomal DNA, and epithelial cell damage was determined by assessing serum levels of intestinal fatty acid-binding protein.ResultsOf 22 Muc2-deficient (Muc2(-/-)) mice, 20 developed AL, compared with seven of 22 control animals (PConclusionA functional mucous layer facilitates the healing of colonic anastomoses.Mucus matters

KW - RISK-FACTORS

KW - GOBLET CELL

KW - GASTROINTESTINAL-TRACT

KW - ANTERIOR RESECTION

KW - COLORECTAL-CANCER

KW - INTESTINAL DAMAGE

KW - MUCUS LAYERS

KW - RAT COLON

KW - LEAKAGE

KW - SURGERY

U2 - 10.1002/bjs.10456

DO - 10.1002/bjs.10456

M3 - Article

SN - 0007-1323

VL - 104

SP - 619

EP - 630

JO - British Journal of Surgery

JF - British Journal of Surgery

IS - 5

ER -