Functional mucous layer and healing of proximal colonic anastomoses in an experimental model

J. W. A. M. Bosmans*, A. C. H. M. Jongen, G. M. H. Birchenough, E. E. L. Nystrom, M. J. J. Gijbels, J. P. M. Derikx, N. D. Bouvy, G. C. Hansson

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Web of Science)

Abstract

BackgroundAnastomotic leakage (AL) is the most dreaded complication after colorectal surgery, causing high morbidity and mortality. Mucus is a first line of defence against external factors in the gastrointestinal tract. In this study, the structural mucus protein Muc2 was depleted in genetically engineered mice and the effect on healing of colonic anastomoses studied in an experimental model.

MethodsMice of different Muc2 genotypes were used in a proximal colonic AL model. Tissues were scored histologically for inflammation, bacterial translocation was determined by quantitative PCR of bacterial 16S ribosomal DNA, and epithelial cell damage was determined by assessing serum levels of intestinal fatty acid-binding protein.

ResultsOf 22 Muc2-deficient (Muc2(-/-)) mice, 20 developed AL, compared with seven of 22 control animals (P

ConclusionA functional mucous layer facilitates the healing of colonic anastomoses.

Mucus matters

Original languageEnglish
Pages (from-to)619-630
Number of pages12
JournalBritish Journal of Surgery
Volume104
Issue number5
DOIs
Publication statusPublished - Apr 2017

Keywords

  • RISK-FACTORS
  • GOBLET CELL
  • GASTROINTESTINAL-TRACT
  • ANTERIOR RESECTION
  • COLORECTAL-CANCER
  • INTESTINAL DAMAGE
  • MUCUS LAYERS
  • RAT COLON
  • LEAKAGE
  • SURGERY

Cite this