Functional annotation of heart enriched mitochondrial genes GBAS and CHCHD10 through guilt by association

Ruben S. R. M. Martherus, Willem Sluiter, Erika D. J. Timmer, Sabina J V Vanherle, Hubert J. M. Smeets, Torik A. Y. Ayoubi*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

28 Citations (Web of Science)

Abstract

Despite the mitochondria ubiquitous nature many of their components display divergences in their expression profile across different tissues. Using the bioinformatics-approach of guilt by association (GBA) we exploited these variations to predict the function of two so far poorly annotated genes: Coiled-coil-helix-coiled-coil-helix domain containing 10 (CHCHD10) and glioblastoma amplified sequence (GBAS). We predicted both genes to be involved in oxidative phosphorylation. Through in vitro experiments using gene-knockdown we could indeed confirm this and furthermore we asserted CHCHD 10 to play a role in complex IV activity.
Original languageEnglish
Pages (from-to)203-208
JournalBiochemical and Biophysical Research Communications
Volume402
Issue number2
DOIs
Publication statusPublished - 12 Nov 2010

Keywords

  • Functional prediction
  • GBAS
  • CHCHD10
  • Complex IV
  • Oxidative phosphorylation

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