Abstract
Despite the mitochondria ubiquitous nature many of their components display divergences in their expression profile across different tissues. Using the bioinformatics-approach of guilt by association (GBA) we exploited these variations to predict the function of two so far poorly annotated genes: Coiled-coil-helix-coiled-coil-helix domain containing 10 (CHCHD10) and glioblastoma amplified sequence (GBAS). We predicted both genes to be involved in oxidative phosphorylation. Through in vitro experiments using gene-knockdown we could indeed confirm this and furthermore we asserted CHCHD 10 to play a role in complex IV activity.
Original language | English |
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Pages (from-to) | 203-208 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 402 |
Issue number | 2 |
DOIs | |
Publication status | Published - 12 Nov 2010 |
Keywords
- Functional prediction
- GBAS
- CHCHD10
- Complex IV
- Oxidative phosphorylation