Functional annotation of heart enriched mitochondrial genes GBAS and CHCHD10 through guilt by association

Ruben S. R. M. Martherus; Willem Sluiter; Erika D. J. Timmer; Sabina J V Vanherle; Hubert J. M. Smeets; Torik A. Y. Ayoubi

doi:10.1016/j.bbrc.2010.09.109

Functional annotation of heart enriched mitochondrial genes GBAS and CHCHD10 through guilt by association

Ruben S. R. M. Martherus, Willem Sluiter, Erika D. J. Timmer, Sabina J V Vanherle, Hubert J. M. Smeets, Torik A. Y. Ayoubi^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

28 Citations (Web of Science)

Abstract

Despite the mitochondria ubiquitous nature many of their components display divergences in their expression profile across different tissues. Using the bioinformatics-approach of guilt by association (GBA) we exploited these variations to predict the function of two so far poorly annotated genes: Coiled-coil-helix-coiled-coil-helix domain containing 10 (CHCHD10) and glioblastoma amplified sequence (GBAS). We predicted both genes to be involved in oxidative phosphorylation. Through in vitro experiments using gene-knockdown we could indeed confirm this and furthermore we asserted CHCHD 10 to play a role in complex IV activity.

Original language	English
Pages (from-to)	203-208
Journal	Biochemical and Biophysical Research Communications
Volume	402
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2010.09.109
Publication status	Published - 12 Nov 2010

Keywords

Functional prediction
GBAS
CHCHD10
Complex IV
Oxidative phosphorylation

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10.1016/j.bbrc.2010.09.109

Cite this

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title = "Functional annotation of heart enriched mitochondrial genes GBAS and CHCHD10 through guilt by association",

abstract = "Despite the mitochondria ubiquitous nature many of their components display divergences in their expression profile across different tissues. Using the bioinformatics-approach of guilt by association (GBA) we exploited these variations to predict the function of two so far poorly annotated genes: Coiled-coil-helix-coiled-coil-helix domain containing 10 (CHCHD10) and glioblastoma amplified sequence (GBAS). We predicted both genes to be involved in oxidative phosphorylation. Through in vitro experiments using gene-knockdown we could indeed confirm this and furthermore we asserted CHCHD 10 to play a role in complex IV activity. ",

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T1 - Functional annotation of heart enriched mitochondrial genes GBAS and CHCHD10 through guilt by association

AU - Martherus, Ruben S. R. M.

AU - Sluiter, Willem

AU - Timmer, Erika D. J.

AU - Vanherle, Sabina J V

AU - Smeets, Hubert J. M.

AU - Ayoubi, Torik A. Y.

PY - 2010/11/12

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AB - Despite the mitochondria ubiquitous nature many of their components display divergences in their expression profile across different tissues. Using the bioinformatics-approach of guilt by association (GBA) we exploited these variations to predict the function of two so far poorly annotated genes: Coiled-coil-helix-coiled-coil-helix domain containing 10 (CHCHD10) and glioblastoma amplified sequence (GBAS). We predicted both genes to be involved in oxidative phosphorylation. Through in vitro experiments using gene-knockdown we could indeed confirm this and furthermore we asserted CHCHD 10 to play a role in complex IV activity.

KW - Functional prediction

KW - GBAS

KW - CHCHD10

KW - Complex IV

KW - Oxidative phosphorylation

U2 - 10.1016/j.bbrc.2010.09.109

DO - 10.1016/j.bbrc.2010.09.109

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VL - 402

SP - 203

EP - 208

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

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