Full-field strain distribution in hierarchical electrospun nanofibrous poly-L(lactic) acid/collagen scaffolds for tendon and ligament regeneration: A multiscale study

Alberto Sensini; Olga Stamati; Gregorio Marchiori; Nicola Sancisi; Carlo Gotti; Gianluca Giavaresi; Luca Cristofolini; Maria Letizia Focarete; Andrea Zucchelli; Gianluca Tozzi

doi:10.1016/j.heliyon.2024.e26796

Full-field strain distribution in hierarchical electrospun nanofibrous poly-L(lactic) acid/collagen scaffolds for tendon and ligament regeneration: A multiscale study

Alberto Sensini^*
, Olga Stamati
, Gregorio Marchiori
, Nicola Sancisi
, Carlo Gotti
, Gianluca Giavaresi
, Luca Cristofolini
, Maria Letizia Focarete
, Andrea Zucchelli^*
, Gianluca Tozzi^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Regeneration of injured tendons and ligaments (T/L) is a worldwide need. In this study electrospun hierarchical scaffolds made of a poly-L (lactic) acid/collagen blend were developed reproducing all the multiscale levels of aggregation of these tissues. Scanning electron microscopy, microCT and tensile mechanical tests were carried out, including a multiscale digital volume correlation analysis to measure the full-field strain distribution of electrospun structures. The principal strains (ε _p1 and ε _p3) described the pattern of strains caused by the nanofibers rearrangement, while the deviatoric strains (ε _D) revealed the related internal sliding of nanofibers and bundles. The results of this study confirmed the biomimicry of such electrospun hierarchical scaffolds, paving the way to further tissue engineering and clinical applications.

Original language	English
Article number	e26796
Journal	Heliyon
Volume	10
Issue number	5
Early online date	15 Mar 2024
DOIs	https://doi.org/10.1016/j.heliyon.2024.e26796
Publication status	Published - 15 Mar 2024

Keywords

Digital volume correlation
Electrospinning
Hierarchical scaffolds
In situ mechanical tests
MicroCT
Tendons and ligaments

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10.1016/j.heliyon.2024.e26796Licence: CC BY

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Sensini, A., Stamati, O., Marchiori, G., Sancisi, N., Gotti, C., Giavaresi, G., Cristofolini, L., Focarete, M. L., Zucchelli, A., & Tozzi, G. (2024). Full-field strain distribution in hierarchical electrospun nanofibrous poly-L(lactic) acid/collagen scaffolds for tendon and ligament regeneration: A multiscale study. Heliyon, 10(5), Article e26796. https://doi.org/10.1016/j.heliyon.2024.e26796

@article{a7a81f28f6a541c2b49bc89a2ae568b0,

title = "Full-field strain distribution in hierarchical electrospun nanofibrous poly-L(lactic) acid/collagen scaffolds for tendon and ligament regeneration: A multiscale study",

abstract = "Regeneration of injured tendons and ligaments (T/L) is a worldwide need. In this study electrospun hierarchical scaffolds made of a poly-L (lactic) acid/collagen blend were developed reproducing all the multiscale levels of aggregation of these tissues. Scanning electron microscopy, microCT and tensile mechanical tests were carried out, including a multiscale digital volume correlation analysis to measure the full-field strain distribution of electrospun structures. The principal strains (ε p1 and ε p3) described the pattern of strains caused by the nanofibers rearrangement, while the deviatoric strains (ε D) revealed the related internal sliding of nanofibers and bundles. The results of this study confirmed the biomimicry of such electrospun hierarchical scaffolds, paving the way to further tissue engineering and clinical applications.",

keywords = "Digital volume correlation, Electrospinning, Hierarchical scaffolds, In situ mechanical tests, MicroCT, Tendons and ligaments",

author = "Alberto Sensini and Olga Stamati and Gregorio Marchiori and Nicola Sancisi and Carlo Gotti and Gianluca Giavaresi and Luca Cristofolini and Focarete, \{Maria Letizia\} and Andrea Zucchelli and Gianluca Tozzi",

note = "Funding Information: The University of Bologna Proof Of Concept Grant and the Horizon Europe Marie Sklodowska Curie Postdoctoral Fellowship 3NTHESES (Grant No. 101061826) are greatly acknowledged. Type I collagen was kindly provided by Kensey Nash Corporation d/b/a DSM Biomedical (Exton, USA). Edward And{\`o} is acknowledged for the useful suggestions in the use of spam software. Ilaria Fuoco and Gaia Prezioso are acknowledged for the help in samples preparation, in the experimental part and data analysis. Publisher Copyright: {\textcopyright} 2024 The Authors",

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doi = "10.1016/j.heliyon.2024.e26796",

language = "English",

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Sensini, A, Stamati, O, Marchiori, G, Sancisi, N, Gotti, C, Giavaresi, G, Cristofolini, L, Focarete, ML, Zucchelli, A & Tozzi, G 2024, 'Full-field strain distribution in hierarchical electrospun nanofibrous poly-L(lactic) acid/collagen scaffolds for tendon and ligament regeneration: A multiscale study', Heliyon, vol. 10, no. 5, e26796. https://doi.org/10.1016/j.heliyon.2024.e26796

TY - JOUR

T1 - Full-field strain distribution in hierarchical electrospun nanofibrous poly-L(lactic) acid/collagen scaffolds for tendon and ligament regeneration

T2 - A multiscale study

AU - Sensini, Alberto

AU - Stamati, Olga

AU - Marchiori, Gregorio

AU - Sancisi, Nicola

AU - Gotti, Carlo

AU - Giavaresi, Gianluca

AU - Cristofolini, Luca

AU - Focarete, Maria Letizia

AU - Zucchelli, Andrea

AU - Tozzi, Gianluca

N1 - Funding Information: The University of Bologna Proof Of Concept Grant and the Horizon Europe Marie Sklodowska Curie Postdoctoral Fellowship 3NTHESES (Grant No. 101061826) are greatly acknowledged. Type I collagen was kindly provided by Kensey Nash Corporation d/b/a DSM Biomedical (Exton, USA). Edward Andò is acknowledged for the useful suggestions in the use of spam software. Ilaria Fuoco and Gaia Prezioso are acknowledged for the help in samples preparation, in the experimental part and data analysis. Publisher Copyright: © 2024 The Authors

PY - 2024/3/15

Y1 - 2024/3/15

N2 - Regeneration of injured tendons and ligaments (T/L) is a worldwide need. In this study electrospun hierarchical scaffolds made of a poly-L (lactic) acid/collagen blend were developed reproducing all the multiscale levels of aggregation of these tissues. Scanning electron microscopy, microCT and tensile mechanical tests were carried out, including a multiscale digital volume correlation analysis to measure the full-field strain distribution of electrospun structures. The principal strains (ε p1 and ε p3) described the pattern of strains caused by the nanofibers rearrangement, while the deviatoric strains (ε D) revealed the related internal sliding of nanofibers and bundles. The results of this study confirmed the biomimicry of such electrospun hierarchical scaffolds, paving the way to further tissue engineering and clinical applications.

AB - Regeneration of injured tendons and ligaments (T/L) is a worldwide need. In this study electrospun hierarchical scaffolds made of a poly-L (lactic) acid/collagen blend were developed reproducing all the multiscale levels of aggregation of these tissues. Scanning electron microscopy, microCT and tensile mechanical tests were carried out, including a multiscale digital volume correlation analysis to measure the full-field strain distribution of electrospun structures. The principal strains (ε p1 and ε p3) described the pattern of strains caused by the nanofibers rearrangement, while the deviatoric strains (ε D) revealed the related internal sliding of nanofibers and bundles. The results of this study confirmed the biomimicry of such electrospun hierarchical scaffolds, paving the way to further tissue engineering and clinical applications.

KW - Digital volume correlation

KW - Electrospinning

KW - Hierarchical scaffolds

KW - In situ mechanical tests

KW - MicroCT

KW - Tendons and ligaments

U2 - 10.1016/j.heliyon.2024.e26796

DO - 10.1016/j.heliyon.2024.e26796

M3 - Article

SN - 2405-8440

VL - 10

JO - Heliyon

JF - Heliyon

IS - 5

M1 - e26796

ER -

Full-field strain distribution in hierarchical electrospun nanofibrous poly-L(lactic) acid/collagen scaffolds for tendon and ligament regeneration: A multiscale study

Abstract

Keywords

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