@article{26c15f4b89284961b19e7afb75d0e5c6,
title = "FT-ICR Mass Spectrometry Imaging at Extreme Mass Resolving Power Using a Dynamically Harmonized ICR Cell with 1 omega or 2 omega Detection",
abstract = "ABSTRACT: MALDI mass spectrometry imaging (MALDI MSI) is a powerful analytical method for achieving 2D localization of compounds from thin sections of typically but not exclusively biological samples. The dynamically harmonized ICR cell (ParaCell) was recently introduced to achieve extreme spectral resolution capable of providing the isotopic fine structure of ions detected in complex samples. The latest improvement in the ICR technology also includes 2 omega detection, which significantly reduces the transient time while preserving the nominal mass resolving power of the ICR cell. High-resolution MS images acquired on FT-ICR instruments equipped with 7T and 9.4T superconducting magnets and the dynamically harmonized ICR cell operating at suboptimal parameters suffered severely from the pixel-to-pixel shifting of m/z peaks due to space-charge effects. The resulting profile average mass spectra have depreciated mass measurement accuracy and mass resolving power under the instrument specifications that affect the confidence level of the identified ions. Here, we propose an analytical workflow based on the monitoring of the total ion current to restrain the pixel-to-pixel m/z shift. Adjustment of the laser parameters is proposed to maintain high spectral resolution and mass accuracy measurement within the instrument specifications during MSI analyses. The optimized method has been successfully employed in replicates to perform high-quality MALDI MS images at resolving power (FWHM) above 1,000,000 in the lipid mass range across the whole image for superconducting magnets of 7T and 9.4T using 1 and 2 omega detection. Our data also compare favorably with MALDI MSI experiments performed on higher-magnetic-field superconducting magnets, including the 21T MALDI FT-ICR prototype instrument of the NHMFL group at Tallahassee, Florida.",
keywords = "SAMPLE PREPARATION, ELECTRIC-FIELD, SPACE-CHARGE, ACCURACY, CALIBRATION, MOTION",
author = "M. Tiquet and {La Rocca}, R. and S. Kirnbauer and S. Zoratto and {Van Kruining}, D. and L. Quinton and G. Eppe and P. Martinez-Martinez and M. Marchetti-Deschmann and {De Pauw}, E. and J. Far",
note = "Funding Information: This work was supported by the Eurlipids Interreg V-A Euregio Meuse-Rhine with support from the European Fund for Regional Development of the European Union (www. eurlipids.com). This work was also a contribution to the EU Horizon 2020 research and Innovation program under grant agreement no. 731077 and the European Project EU_FTICR_MS (H2020 INFRAIA-02-2017). The MALDI-ToF rapifleX and the MALDI FT-ICR solariX XR were co-funded by FEDER BIOMED HUB Technology Support (number 2.2.1/996). This work was also a contribution to ZonMw Memorabel program (number: 733050105), Cost action under grant CA16122 − BIONECA and Hersenstichting (number: DR-2018-00274). The scimaX 7T 2XR was funded by TU Wien and the Federal Ministery Republic of Austria for Education, Science and Research (HRSM2016). This work was also a contribution to the Christian Doppler Laboratory for Skin Multimodal Imaging of Aging and Senescence (SkinMAGINE) and the Austrian Science Foundation project ChemTalk (P32179-B). All experiments were performed with permission from the Committee on Animal Welfare of Maastricht University and the Committee on Animal Welfare of Liege University, according to Dutch or Belgian governmental legislation, respectively. Funding Information: This work was supported by the Eurlipids Interreg V-A Euregio Meuse-Rhine with support from the European Fund for Regional Development of the European Union (www. eurlipids.com). This work was also a contribution to the EU Horizon 2020 research and Innovation program under grant agreement no. 731077 and the European Project EU_FT-ICR_MS (H2020 INFRAIA-02-2017). The MALDI-ToF rapifleX and the MALDI FT-ICR solariX XR were co-funded by FEDER BIOMED HUB Technology Support (number 2.2.1/996). This work was also a contribution to ZonMw Memorabel program (number: 733050105), Cost action under grant CA16122 − BIONECA and Hersenstichting (number: DR-2018-00274). The scimaX 7T 2XR was funded by TU Wien and the Federal Ministery Republic of Austria for Education, Science and Research (HRSM2016). This work was also a contribution to the Christian Doppler Laboratory for Skin Multimodal Imaging of Aging and Senescence (SkinMAGINE) and the Austrian Science Foundation project ChemTalk (P32179-B). All experiments were performed with permission from the Committee on Animal Welfare of Maastricht University and the Committee on Animal Welfare of Liege University, according to Dutch or Belgian governmental legislation, respectively. Publisher Copyright: {\textcopyright} 2022 The Authors. Published by American Chemical Society.",
year = "2022",
month = jul,
day = "5",
doi = "10.1021/acs.analchem.2c00754",
language = "English",
volume = "94",
pages = "9316--9326",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "26",
}