Free radicals in exhaled breath condensate in cystic fibrosis and healthy subjects

P.P.R. Rosias; G.J. den Hartog; C.M. Robroeks; A. Bast; R.A. Donckerwolcke; J.W. Heynens; J. Suykerbuyk; H.J. Hendriks; Q. Jöbsis; E.D. Dompeling

doi:10.1080/10715760500522648

Free radicals in exhaled breath condensate in cystic fibrosis and healthy subjects

P.P.R. Rosias^*, G.J. den Hartog, C.M. Robroeks, A. Bast, R.A. Donckerwolcke, J.W. Heynens, J. Suykerbuyk, H.J. Hendriks, Q. Jöbsis, E.D. Dompeling

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Many markers of airway inflammation and oxidative stress can be measured non-invasively in exhaled breath condensate (EBC). However, no attempt has been made to directly detect free radicals using electron paramagnetic resonance (EPR) spectroscopy. Condensate was collected in 14 children with cystic fibrosis (CF) and seven healthy subjects. Free radicals were trapped by 5,5-dimethyl-1-pyrroline-N-oxide. EPR spectra were recorded using a Bruker EMX spectrometer. Secondly, to study the source of oxygen centered radical formation, catalase or hydrogen peroxide was added to the condensate. Radicals were detected in 18 out of 21 condensate samples. Analysis of spectra indicated that both oxygen and carbon centered radicals were trapped. Within-subject reproducibility was good in all but one subject. Quantitatively, there was a trend towards higher maximal peak heights of both oxygen and carbon centered radicals in the children with CF. Catalase completely suppressed the signals in condensate. Addition of hydrogen peroxide resulted in increased radical signal intensity. Detection of free radicals in EBC of children with CF and healthy subjects is feasible using EPR spectroscopy.

Original language	English
Pages (from-to)	901-909
Journal	Free Radical Research
Volume	40
Issue number	9
DOIs	https://doi.org/10.1080/10715760500522648
Publication status	Published - 1 Jan 2006

Access to Document

10.1080/10715760500522648

Cite this

@article{5e43e661971a49dab6a9717ae8ae6002,

title = "Free radicals in exhaled breath condensate in cystic fibrosis and healthy subjects",

abstract = "Many markers of airway inflammation and oxidative stress can be measured non-invasively in exhaled breath condensate (EBC). However, no attempt has been made to directly detect free radicals using electron paramagnetic resonance (EPR) spectroscopy. Condensate was collected in 14 children with cystic fibrosis (CF) and seven healthy subjects. Free radicals were trapped by 5,5-dimethyl-1-pyrroline-N-oxide. EPR spectra were recorded using a Bruker EMX spectrometer. Secondly, to study the source of oxygen centered radical formation, catalase or hydrogen peroxide was added to the condensate. Radicals were detected in 18 out of 21 condensate samples. Analysis of spectra indicated that both oxygen and carbon centered radicals were trapped. Within-subject reproducibility was good in all but one subject. Quantitatively, there was a trend towards higher maximal peak heights of both oxygen and carbon centered radicals in the children with CF. Catalase completely suppressed the signals in condensate. Addition of hydrogen peroxide resulted in increased radical signal intensity. Detection of free radicals in EBC of children with CF and healthy subjects is feasible using EPR spectroscopy.",

author = "P.P.R. Rosias and {den Hartog}, G.J. and C.M. Robroeks and A. Bast and R.A. Donckerwolcke and J.W. Heynens and J. Suykerbuyk and H.J. Hendriks and Q. J{\"o}bsis and E.D. Dompeling",

year = "2006",

month = jan,

day = "1",

doi = "10.1080/10715760500522648",

language = "English",

volume = "40",

pages = "901--909",

journal = "Free Radical Research",

issn = "1071-5762",

publisher = "Informa Healthcare",

number = "9",

}

TY - JOUR

T1 - Free radicals in exhaled breath condensate in cystic fibrosis and healthy subjects

AU - Rosias, P.P.R.

AU - den Hartog, G.J.

AU - Robroeks, C.M.

AU - Bast, A.

AU - Donckerwolcke, R.A.

AU - Heynens, J.W.

AU - Suykerbuyk, J.

AU - Hendriks, H.J.

AU - Jöbsis, Q.

AU - Dompeling, E.D.

PY - 2006/1/1

Y1 - 2006/1/1

N2 - Many markers of airway inflammation and oxidative stress can be measured non-invasively in exhaled breath condensate (EBC). However, no attempt has been made to directly detect free radicals using electron paramagnetic resonance (EPR) spectroscopy. Condensate was collected in 14 children with cystic fibrosis (CF) and seven healthy subjects. Free radicals were trapped by 5,5-dimethyl-1-pyrroline-N-oxide. EPR spectra were recorded using a Bruker EMX spectrometer. Secondly, to study the source of oxygen centered radical formation, catalase or hydrogen peroxide was added to the condensate. Radicals were detected in 18 out of 21 condensate samples. Analysis of spectra indicated that both oxygen and carbon centered radicals were trapped. Within-subject reproducibility was good in all but one subject. Quantitatively, there was a trend towards higher maximal peak heights of both oxygen and carbon centered radicals in the children with CF. Catalase completely suppressed the signals in condensate. Addition of hydrogen peroxide resulted in increased radical signal intensity. Detection of free radicals in EBC of children with CF and healthy subjects is feasible using EPR spectroscopy.

AB - Many markers of airway inflammation and oxidative stress can be measured non-invasively in exhaled breath condensate (EBC). However, no attempt has been made to directly detect free radicals using electron paramagnetic resonance (EPR) spectroscopy. Condensate was collected in 14 children with cystic fibrosis (CF) and seven healthy subjects. Free radicals were trapped by 5,5-dimethyl-1-pyrroline-N-oxide. EPR spectra were recorded using a Bruker EMX spectrometer. Secondly, to study the source of oxygen centered radical formation, catalase or hydrogen peroxide was added to the condensate. Radicals were detected in 18 out of 21 condensate samples. Analysis of spectra indicated that both oxygen and carbon centered radicals were trapped. Within-subject reproducibility was good in all but one subject. Quantitatively, there was a trend towards higher maximal peak heights of both oxygen and carbon centered radicals in the children with CF. Catalase completely suppressed the signals in condensate. Addition of hydrogen peroxide resulted in increased radical signal intensity. Detection of free radicals in EBC of children with CF and healthy subjects is feasible using EPR spectroscopy.

U2 - 10.1080/10715760500522648

DO - 10.1080/10715760500522648

M3 - Article

SN - 1071-5762

VL - 40

SP - 901

EP - 909

JO - Free Radical Research

JF - Free Radical Research

IS - 9

ER -