Flow cytometric mepacrine fluorescence can be used for the exclusion of platelet dense granule deficiency

I. van Asten, M. Blaauwgeers, L. Granneman, H.F.G. Heijnen, M.J.H.A. Kruip, E.A.M. Beckers, M. Coppens, J. Eikenboom, R.Y.J. Tamminga, G. Pasterkamp, A. Huisman, K.P.M. van Galen, S.J.A. Korporaal, R.E.G. Schutgens, R.T. Urbanus*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Background delta-storage pool disease (delta-SPD) is a bleeding disorder characterized by a reduced number of platelet-dense granules. The diagnosis of delta-SPD depends on the measurement of platelet ADP content, but this test is time consuming and requires a relatively large blood volume. Flow cytometric analysis of platelet mepacrine uptake is a potential alternative, but this approach lacks validation, which precludes its use in a diagnostic setting.Objectives To evaluate the performance of platelet mepacrine uptake as a diagnostic test for delta-SPD.Patients/Methods Mepacrine fluorescence was determined with flow cytometry before and after platelet activation in 156 patients with a suspected platelet function disorder and compared with platelet ADP content as a reference test. Performance was analyzed with a receiver operating characteristic (ROC) curve.Results Eleven of 156 patients had delta-SPD based on platelet ADP content. Mepacrine fluorescence was inferior to platelet ADP content in identifying patients with delta-SPD, but both mepacrine uptake (area under the ROC curve [AUC] 0.87) and mepacrine release after platelet activation (AUC 0.80) had good discriminative ability. In our tertiary reference center, mepacrine uptake showed high negative predicitive value (97%) with low positive predictive value (35%). Combined with a negative likelihood ratio of 0.1, these data indicate that mepacrine uptake can be used to exclude delta-SPD in patients with a bleeding tendency.Conclusion Mepacrine fluorescence can be used as a screening tool to exclude delta-SPD in a large number of patients with a suspected platelet function disorder.
Original languageEnglish
Pages (from-to)706-713
Number of pages8
JournalJournal of Thrombosis and Haemostasis
Volume18
Issue number3
DOIs
Publication statusPublished - 1 Mar 2020

Keywords

  • assay
  • blood platelets
  • diagnosis
  • disorders
  • flow cytometry
  • platelet function testing
  • platelet storage pool deficiency
  • quinacrine
  • storage pool deficiency
  • STORAGE POOL DEFICIENCY
  • DIAGNOSIS
  • ASSAY
  • DISORDERS

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