Flow cytometric analysis of platelet function to improve the recognition of thrombocytopathy

Dana Huskens; Li Li; Lisa Florin; Pieter de Kesel; Bas de Laat; Mark Roest; Katrien M. J. Devreese

doi:10.1016/j.thromres.2020.06.037

Flow cytometric analysis of platelet function to improve the recognition of thrombocytopathy

Dana Huskens^*, Li Li, Lisa Florin, Pieter de Kesel, Bas de Laat, Mark Roest, Katrien M. J. Devreese

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Introduction: Light transmission aggregometry (LTA) is the gold standard for diagnosing bleeding disorders. Although LTA is laborious, requires large volumes of blood and is relatively insensitive to small changes in platelet function, there is still no competing alternative approach to replace LTA for the diagnosis of platelet bleeding disorders.

Materials and methods: This study investigates the correlation between flow cytometry-based whole blood platelet activation test (WB-PACT) and LTA and whether WB-PACT is of additional value for the identification of bleeding disorders. In total, 161 patients with suspected bleeding diathesis were tested.

Results: A correlation of 0.41 between LTA and WB-PACT was found, and there was agreement between tests in 62% of cases (kappa = 0.23). The WB-PACT is of additional value to LTA to detect platelet function disorders (PFD) as 10 patients with elevated bleeding score (BS) were detected with WB-PACT, 4 with LTA and 7 patients were positive with both tests. Interestingly, in contrast to LTA, WB-PACT has an additional option to detect VWF disfunctions.

Conclusion: WB-PACT may have added value for the routine diagnostic work-up in patients who need to have platelet function tested.

Original language	English
Pages (from-to)	183-189
Number of pages	7
Journal	Thrombosis Research
Volume	194
DOIs	https://doi.org/10.1016/j.thromres.2020.06.037
Publication status	Published - Oct 2020

Keywords

Platelet function defect
Flow cytometry
Aggregometry
BLEEDING ASSESSMENT-TOOL
FUNCTION DISORDERS
GLYCOPROTEIN-VI
THROMBUS FORMATION
AGGREGOMETRY
VALIDATION
DIAGNOSIS
CONSENSUS

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@article{39f5862e499d4f18bf59b5b7641e8143,

title = "Flow cytometric analysis of platelet function to improve the recognition of thrombocytopathy",

abstract = "Introduction: Light transmission aggregometry (LTA) is the gold standard for diagnosing bleeding disorders. Although LTA is laborious, requires large volumes of blood and is relatively insensitive to small changes in platelet function, there is still no competing alternative approach to replace LTA for the diagnosis of platelet bleeding disorders.Materials and methods: This study investigates the correlation between flow cytometry-based whole blood platelet activation test (WB-PACT) and LTA and whether WB-PACT is of additional value for the identification of bleeding disorders. In total, 161 patients with suspected bleeding diathesis were tested.Results: A correlation of 0.41 between LTA and WB-PACT was found, and there was agreement between tests in 62% of cases (kappa = 0.23). The WB-PACT is of additional value to LTA to detect platelet function disorders (PFD) as 10 patients with elevated bleeding score (BS) were detected with WB-PACT, 4 with LTA and 7 patients were positive with both tests. Interestingly, in contrast to LTA, WB-PACT has an additional option to detect VWF disfunctions.Conclusion: WB-PACT may have added value for the routine diagnostic work-up in patients who need to have platelet function tested.",

keywords = "Platelet function defect, Flow cytometry, Aggregometry, BLEEDING ASSESSMENT-TOOL, FUNCTION DISORDERS, GLYCOPROTEIN-VI, THROMBUS FORMATION, AGGREGOMETRY, VALIDATION, DIAGNOSIS, CONSENSUS",

author = "Dana Huskens and Li Li and Lisa Florin and Kesel, {Pieter de} and Laat, {Bas de} and Mark Roest and Devreese, {Katrien M. J.}",

note = "Funding Information: L.L. was supported by the China Scholarship Council (CSC) via the State Scholarship Fund (File No. 201706230245 ). Publisher Copyright: {\textcopyright} 2020 The Author(s)",

year = "2020",

month = oct,

doi = "10.1016/j.thromres.2020.06.037",

language = "English",

volume = "194",

pages = "183--189",

journal = "Thrombosis Research",

issn = "0049-3848",

publisher = "Elsevier Science",

}

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T1 - Flow cytometric analysis of platelet function to improve the recognition of thrombocytopathy

AU - Huskens, Dana

AU - Li, Li

AU - Florin, Lisa

AU - Kesel, Pieter de

AU - Laat, Bas de

AU - Roest, Mark

AU - Devreese, Katrien M. J.

PY - 2020/10

Y1 - 2020/10

N2 - Introduction: Light transmission aggregometry (LTA) is the gold standard for diagnosing bleeding disorders. Although LTA is laborious, requires large volumes of blood and is relatively insensitive to small changes in platelet function, there is still no competing alternative approach to replace LTA for the diagnosis of platelet bleeding disorders.Materials and methods: This study investigates the correlation between flow cytometry-based whole blood platelet activation test (WB-PACT) and LTA and whether WB-PACT is of additional value for the identification of bleeding disorders. In total, 161 patients with suspected bleeding diathesis were tested.Results: A correlation of 0.41 between LTA and WB-PACT was found, and there was agreement between tests in 62% of cases (kappa = 0.23). The WB-PACT is of additional value to LTA to detect platelet function disorders (PFD) as 10 patients with elevated bleeding score (BS) were detected with WB-PACT, 4 with LTA and 7 patients were positive with both tests. Interestingly, in contrast to LTA, WB-PACT has an additional option to detect VWF disfunctions.Conclusion: WB-PACT may have added value for the routine diagnostic work-up in patients who need to have platelet function tested.

AB - Introduction: Light transmission aggregometry (LTA) is the gold standard for diagnosing bleeding disorders. Although LTA is laborious, requires large volumes of blood and is relatively insensitive to small changes in platelet function, there is still no competing alternative approach to replace LTA for the diagnosis of platelet bleeding disorders.Materials and methods: This study investigates the correlation between flow cytometry-based whole blood platelet activation test (WB-PACT) and LTA and whether WB-PACT is of additional value for the identification of bleeding disorders. In total, 161 patients with suspected bleeding diathesis were tested.Results: A correlation of 0.41 between LTA and WB-PACT was found, and there was agreement between tests in 62% of cases (kappa = 0.23). The WB-PACT is of additional value to LTA to detect platelet function disorders (PFD) as 10 patients with elevated bleeding score (BS) were detected with WB-PACT, 4 with LTA and 7 patients were positive with both tests. Interestingly, in contrast to LTA, WB-PACT has an additional option to detect VWF disfunctions.Conclusion: WB-PACT may have added value for the routine diagnostic work-up in patients who need to have platelet function tested.

KW - Platelet function defect

KW - Flow cytometry

KW - Aggregometry

KW - BLEEDING ASSESSMENT-TOOL

KW - FUNCTION DISORDERS

KW - GLYCOPROTEIN-VI

KW - THROMBUS FORMATION

KW - AGGREGOMETRY

KW - VALIDATION

KW - DIAGNOSIS

KW - CONSENSUS

U2 - 10.1016/j.thromres.2020.06.037

DO - 10.1016/j.thromres.2020.06.037

M3 - Article

C2 - 32788114

SN - 0049-3848

VL - 194

SP - 183

EP - 189

JO - Thrombosis Research

JF - Thrombosis Research

ER -

Flow cytometric analysis of platelet function to improve the recognition of thrombocytopathy

Abstract

Keywords

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