Flow cytometric analysis of myelodysplasia: Pre-analytical and technical issues-Recommendations from the European LeukemiaNet

V.H.J. van der Velden*, F. Preijers, U. Johansson, T.M. Westers, A. Dunlop, A. Porwit, M.C. Bene, P. Valent, J. te Marvelde, O. Wagner-Ballon, U. Oelschlaegel, L. Saft, S. Kordasti, R. Ireland, E. Cremers, C. Alhan, C. Duetz, W. Hobo, N. Chapuis, M. FontenayP. Bettelheim, L. Eidenshink-Brodersen, P. Font, M.R. Loken, S. Matarraz, K. Ogata, A. Orfao, K. Psarra, D. Subira, D.A. Wells, M.G. Della Porta, K. Burbury, F. Bellos, E. Weiss, W. Kern, A. van de Loosdrecht

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Background Flow cytometry (FCM) aids the diagnosis and prognostic stratification of patients with suspected or confirmed myelodysplastic syndrome (MDS). Over the past few years, significant progress has been made in the FCM field concerning technical issues (including software and hardware) and pre-analytical procedures. Methods Recommendations are made based on the data and expert discussions generated from 13 yearly meetings of the European LeukemiaNet international MDS Flow working group. Results We report here on the experiences and recommendations concerning (1) the optimal methods of sample processing and handling, (2) antibody panels and fluorochromes, and (3) current hardware technologies. Conclusions These recommendations will support and facilitate the appropriate application of FCM assays in the diagnostic workup of MDS patients. Further standardization and harmonization will be required to integrate FCM in MDS diagnostic evaluations in daily practice.
Original languageEnglish
Pages (from-to)15-26
Number of pages12
JournalCytometry Part B-clinical Cytometry
Volume104
Issue number1
Early online date11 Dec 2021
DOIs
Publication statusPublished - Jan 2023

Keywords

  • ELN
  • flow cytometry
  • MDS
  • pre-analytic issues
  • BONE-MARROW
  • REFRACTORY CYTOPENIA
  • CONSENSUS STATEMENTS
  • DIAGNOSTIC-CRITERIA
  • LOW-GRADE
  • CLASSIFICATION
  • MASTOCYTOSIS
  • STANDARDS
  • QUANTIFICATION

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