Fixation methods for electron microscopy of human and other liver

E. Eddie Wisse; F. Braet; H. Duimel; C. Vreuls; G.H. Koek; S. Olde Damink; M.A. van den Broek; B. De Geest; C.H.C. Dejong; C. Tateno; P. Frederik

doi:10.3748/wjg.v16.i23.2851

Fixation methods for electron microscopy of human and other liver

E. Eddie Wisse^*, F. Braet, H. Duimel, C. Vreuls, G.H. Koek, S. Olde Damink, M.A. van den Broek, B. De Geest, C.H.C. Dejong, C. Tateno, P. Frederik

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

For an electron microscopic study of the liver, expertise and complicated, time-consuming processing of hepatic tissues and cells is needed. The interpretation of electron microscopy (EM) images requires knowledge of the liver fine structure and experience with the numerous artifacts in fixation, embedding, sectioning, contrast staining and microscopic imaging. Hence, the aim of this paper is to present a detailed summary of different methods for the preparation of hepatic cells and tissue, for the purpose of preserving long-standing expertise and to encourage new investigators and clinicians to include EM studies of liver cells and tissue in their projects.

Original language	English
Pages (from-to)	2851-66
Journal	World Journal of Gastroenterology
Volume	16
Issue number	23
DOIs	https://doi.org/10.3748/wjg.v16.i23.2851
Publication status	Published - 1 Jan 2010

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10.3748/wjg.v16.i23.2851Licence: CC BY-NC

Cite this

@article{ab005a91bc7d4ac088b8a714fd701078,

title = "Fixation methods for electron microscopy of human and other liver",

abstract = "For an electron microscopic study of the liver, expertise and complicated, time-consuming processing of hepatic tissues and cells is needed. The interpretation of electron microscopy (EM) images requires knowledge of the liver fine structure and experience with the numerous artifacts in fixation, embedding, sectioning, contrast staining and microscopic imaging. Hence, the aim of this paper is to present a detailed summary of different methods for the preparation of hepatic cells and tissue, for the purpose of preserving long-standing expertise and to encourage new investigators and clinicians to include EM studies of liver cells and tissue in their projects.",

author = "{Eddie Wisse}, E. and F. Braet and H. Duimel and C. Vreuls and G.H. Koek and {Olde Damink}, S. and {van den Broek}, M.A. and {De Geest}, B. and C.H.C. Dejong and C. Tateno and P. Frederik",

year = "2010",

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doi = "10.3748/wjg.v16.i23.2851",

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TY - JOUR

T1 - Fixation methods for electron microscopy of human and other liver

AU - Eddie Wisse, E.

AU - Braet, F.

AU - Duimel, H.

AU - Vreuls, C.

AU - Koek, G.H.

AU - Olde Damink, S.

AU - van den Broek, M.A.

AU - De Geest, B.

AU - Dejong, C.H.C.

AU - Tateno, C.

AU - Frederik, P.

PY - 2010/1/1

Y1 - 2010/1/1

N2 - For an electron microscopic study of the liver, expertise and complicated, time-consuming processing of hepatic tissues and cells is needed. The interpretation of electron microscopy (EM) images requires knowledge of the liver fine structure and experience with the numerous artifacts in fixation, embedding, sectioning, contrast staining and microscopic imaging. Hence, the aim of this paper is to present a detailed summary of different methods for the preparation of hepatic cells and tissue, for the purpose of preserving long-standing expertise and to encourage new investigators and clinicians to include EM studies of liver cells and tissue in their projects.

AB - For an electron microscopic study of the liver, expertise and complicated, time-consuming processing of hepatic tissues and cells is needed. The interpretation of electron microscopy (EM) images requires knowledge of the liver fine structure and experience with the numerous artifacts in fixation, embedding, sectioning, contrast staining and microscopic imaging. Hence, the aim of this paper is to present a detailed summary of different methods for the preparation of hepatic cells and tissue, for the purpose of preserving long-standing expertise and to encourage new investigators and clinicians to include EM studies of liver cells and tissue in their projects.

U2 - 10.3748/wjg.v16.i23.2851

DO - 10.3748/wjg.v16.i23.2851

M3 - Article

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SN - 1007-9327

VL - 16

SP - 2851

EP - 2866

JO - World Journal of Gastroenterology

JF - World Journal of Gastroenterology

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