Fixation methods for electron microscopy of human and other liver

E. Eddie Wisse*, F. Braet, H. Duimel, C. Vreuls, G.H. Koek, S. Olde Damink, M.A. van den Broek, B. De Geest, C.H.C. Dejong, C. Tateno, P. Frederik

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


For an electron microscopic study of the liver, expertise and complicated, time-consuming processing of hepatic tissues and cells is needed. The interpretation of electron microscopy (EM) images requires knowledge of the liver fine structure and experience with the numerous artifacts in fixation, embedding, sectioning, contrast staining and microscopic imaging. Hence, the aim of this paper is to present a detailed summary of different methods for the preparation of hepatic cells and tissue, for the purpose of preserving long-standing expertise and to encourage new investigators and clinicians to include EM studies of liver cells and tissue in their projects.
Original languageEnglish
Pages (from-to)2851-66
JournalWorld Journal of Gastroenterology
Issue number23
Publication statusPublished - 1 Jan 2010

Cite this