Feasibility of a new method to collect exhaled breath condensate in pre-school children.

P.P.R. Rosias, C.M. Robroeks, K.D.G. van de Kant, G.T. Rijkers, L.J.I. Zimmermann, C.P. van Schayck, Q. Jöbsis, E.D. Dompeling

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Abstract

Exhaled breath condensate (EBC) is a promising non-invasive method to assess respiratory inflammation in adults and children with lung disease. Especially in pre-school children, condensate collection is hampered by long sampling times because of open-ended collection systems. We aimed to assess the feasibility of condensate collection in pre-school children using a closed glass condenser with breath recirculation system, which also collects the residual non-condensed exhaled breath, and subsequently recirculates it back into the condenser. Condensate was collected before and after breath recirculation in 70 non-sedated pre-school children with and without recurrent wheeze. Cytokines (IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-13, TNF-alpha) were measured in 50 mu l samples using ultrasensitive multiplexed liquid bead array. The success rate of condensate collection increased from 64% (without recirculation) to 83% (after breath recirculation), and mean condensate volume from 214 to 465 mu l respectively. Detection of cytokines was successful in 95-100% of samples. Cytokine concentrations before and after breath recirculation were not different (p > 0.232). In asthmatic children, only TNF-a concentrations were significantly decreased, compared to non-asthmatics. In pre-school children, the collection of EBC is feasible using a new closed glass condenser with breath recirculation system. This new method may help to assess - non-invasively - cytokine profiles in asthmatic and non-asthmatic pre-school children.

Original languageEnglish
Pages (from-to)E235-E244
Number of pages10
JournalPediatric Allergy and Immunology
Volume21
Issue number1
DOIs
Publication statusPublished - Feb 2010

Keywords

  • asthma
  • cytokines
  • exhaled breath condensate
  • muliplex bead array
  • pre-school children
  • wheezing
  • HYDROGEN-PEROXIDE
  • CYSTIC-FIBROSIS
  • ASTHMA
  • CYTOKINES
  • BIOMARKERS
  • IMMUNOASSAY
  • EICOSANOIDS

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