TY - JOUR
T1 - NAD+-Precursor Supplementation With L-Tryptophan, Nicotinic Acid, and Nicotinamide Does Not Affect Mitochondrial Function or Skeletal Muscle Function in Physically Compromised Older Adults
AU - Connell, N.J.
AU - Grevendonk, L.
AU - Fealy, C.E.
AU - Moonen-Kornips, E.
AU - Bruls, Y.M.H.
AU - Schrauwen-Hinderling, V.B.
AU - de Vogel, J.
AU - Hageman, R.
AU - Geurts, J.
AU - Zapata-Perez, R.
AU - Houtkooper, R.H.
AU - Havekes, B.
AU - Hoeks, J.
AU - Schrauwen, P.
N1 - Funding Information:
This work was supported by the Top Institute Food and Nutrition (TIFN) research program on Mitochondrial Health under grant number 16NH01.
Publisher Copyright:
© The Author(s) 2021.
PY - 2021/10/1
Y1 - 2021/10/1
N2 - Background: Boosting NAD(+) via supplementation with niacin equivalents has been proposed as a potential modality capable of promoting healthy aging and negating age-dependent declines of skeletal muscle mass and function.Objectives: We investigated the efficacy of NAD(+)-precursor supplementation (tryptophan, nicotinic acid, and nicotinamide) on skeletal muscle mitochondrial function in physically compromised older adults.Methods: A randomized, double-blind, controlled trial was conducted in 14 (female/male: 4/10) community-dwelling, older adults with impaired physical function [age, 72.9 +/- 4.0 years; BMI, 25.2 +/- 2.3 kg/m(2)]. Participants were supplemented with 207.5 mg niacin equivalents/day [intervention (INT)] and a control product (CON) that did not contain niacin equivalents, each for 32 days. The primary outcomes tested were mitochondrial oxidative capacity and exercise efficiency, analyzed by means of paired Student's t-tests. Secondary outcomes, such as NAD(+) concentrations, were analyzed accordingly.Results: Following supplementation, skeletal muscle NAD(+) concentrations [7.5 +/- 1.9 compared with 7.9 +/- 1.6 AU, respectively] in INT compared with CON conditions were not significantly different compared to the control condition, whereas skeletal muscle methyl-nicotinamide levels were significantly higher under NAD(+)-precursor supplementation [INT, 0.098 +/- 0.063 compared with CON, 0.025 +/- 0.014; P = 0.001], suggesting an increased NAD(+) metabolism. Conversely, neither ADP-stimulated [INT, 82.1 +/- 19.0 compared with CON, 84.0 +/- 19.2; P = 0.716] nor maximally uncoupled mitochondrial respiration [INT, 103.4 +/- 30.7 compared with CON, 108.7 +/- 33.4; P = 0.495] improved under NAD(+)-precursor supplementation, nor did net exercise efficiency during the submaximal cycling test [INT, 20.2 +/- 2.77 compared with CON, 20.8 +/- 2.88; P = 0.342].Conclusions: Our findings are consistent with previous findings on NAD(+) efficacy in humans, and we show in community-dwelling, older adults with impaired physical function that NAD(+)-precursor supplementation through L-tryptophan, nicotinic acid, and nicotinamide does not improve mitochondrial or skeletal muscle function.
AB - Background: Boosting NAD(+) via supplementation with niacin equivalents has been proposed as a potential modality capable of promoting healthy aging and negating age-dependent declines of skeletal muscle mass and function.Objectives: We investigated the efficacy of NAD(+)-precursor supplementation (tryptophan, nicotinic acid, and nicotinamide) on skeletal muscle mitochondrial function in physically compromised older adults.Methods: A randomized, double-blind, controlled trial was conducted in 14 (female/male: 4/10) community-dwelling, older adults with impaired physical function [age, 72.9 +/- 4.0 years; BMI, 25.2 +/- 2.3 kg/m(2)]. Participants were supplemented with 207.5 mg niacin equivalents/day [intervention (INT)] and a control product (CON) that did not contain niacin equivalents, each for 32 days. The primary outcomes tested were mitochondrial oxidative capacity and exercise efficiency, analyzed by means of paired Student's t-tests. Secondary outcomes, such as NAD(+) concentrations, were analyzed accordingly.Results: Following supplementation, skeletal muscle NAD(+) concentrations [7.5 +/- 1.9 compared with 7.9 +/- 1.6 AU, respectively] in INT compared with CON conditions were not significantly different compared to the control condition, whereas skeletal muscle methyl-nicotinamide levels were significantly higher under NAD(+)-precursor supplementation [INT, 0.098 +/- 0.063 compared with CON, 0.025 +/- 0.014; P = 0.001], suggesting an increased NAD(+) metabolism. Conversely, neither ADP-stimulated [INT, 82.1 +/- 19.0 compared with CON, 84.0 +/- 19.2; P = 0.716] nor maximally uncoupled mitochondrial respiration [INT, 103.4 +/- 30.7 compared with CON, 108.7 +/- 33.4; P = 0.495] improved under NAD(+)-precursor supplementation, nor did net exercise efficiency during the submaximal cycling test [INT, 20.2 +/- 2.77 compared with CON, 20.8 +/- 2.88; P = 0.342].Conclusions: Our findings are consistent with previous findings on NAD(+) efficacy in humans, and we show in community-dwelling, older adults with impaired physical function that NAD(+)-precursor supplementation through L-tryptophan, nicotinic acid, and nicotinamide does not improve mitochondrial or skeletal muscle function.
KW - NAD plus -precursors
KW - skeletal muscle
KW - mitochondria! function
KW - older adults
KW - metabolism
KW - muscle health
KW - FATTY LIVER-DISEASE
KW - ADENINE-DINUCLEOTIDE
KW - OXIDATIVE CAPACITY
KW - THERAPEUTIC TARGET
KW - NAD(+) DEFICIENCY
KW - LIFE-SPAN
KW - METABOLISM
KW - PERFORMANCE
KW - RIBOSIDE
KW - MONONUCLEOTIDE
U2 - 10.1093/jn/nxab193
DO - 10.1093/jn/nxab193
M3 - Article
C2 - 34191033
SN - 0022-3166
VL - 151
SP - 2917
EP - 2931
JO - Journal of Nutrition
JF - Journal of Nutrition
IS - 10
ER -