TY - JOUR
T1 - Fabrication of hybrid scaffolds obtained from combinations of PCL with gelatin or collagen via electrospinning for skeletal muscle tissue engineering
AU - Perez-Puyana, Victor
AU - Wieringa, Paul
AU - Yuste, Yaiza
AU - de la Portilla, Fernando
AU - Guererro, Antonio
AU - Romero, Alberto
AU - Moroni, Lorenzo
N1 - Funding Information:
This work is part of a research project sponsored by “Ministerio de Economía y Competitividad” (MINECO/FEDER, EU) from the Spanish Government (Ref. CTQ2015‐71164‐P). The authors gratefully acknowledge their financial support. The authors also acknowledge Universidad de Sevilla for the VPPI‐US grant of Victor M. Perez‐Puyana. Part of this work was carried out at the Department of Complex Tissue Regeneration (MERLN Institute for Technology‐Inspired Regenerative Medicine, Maastricht University) and with financial support from the program from the Universidad de Sevilla. This research project was made possible by the Dutch Province of Limburg (LINK program). “Estancias breves en España y en el extranjero para beneficiarios de Becas predoctorales o PIF de la US y de Becas de la Fundación Cámara”
Funding Information:
This work is part of a research project sponsored by ?Ministerio de Econom?a y Competitividad? (MINECO/FEDER, EU) from the Spanish Government (Ref. CTQ2015-71164-P). The authors gratefully acknowledge their financial support. The authors also acknowledge Universidad de Sevilla for the VPPI-US grant of Victor M. Perez-Puyana. Part of this work was carried out at the Department of Complex Tissue Regeneration (MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University) and with financial support from the program ?Estancias breves en Espa?a y en el extranjero para beneficiarios de Becas predoctorales o PIF de la US y de Becas de la Fundaci?n C?mara? from the Universidad de Sevilla. This research project was made possible by the Dutch Province of Limburg (LINK program).
Publisher Copyright:
© 2021 The Authors. Journal of Biomedical Materials Research Part A published by Wiley Periodicals LLC.
PY - 2021/9
Y1 - 2021/9
N2 - The creation of skeletal muscle tissue in vitro is a major topic of interest today in the field of biomedical research, due to the lack of treatments for muscle loss due to traumatic accidents or disease. For this reason, the intrinsic properties of nanofibrillar structures to promote cell adhesion, proliferation, and cell alignment presents an attractive tool for regenerative medicine to recreate organized tissues such as muscle. Electrospinning is one of the processing techniques often used for the fabrication of these nanofibrous structures and the combination of synthetic and natural polymers is often required to achieve optimal mechanical and physiochemical properties. Here, polycaprolactone (PCL) is selected as a synthetic polymer used for the fabrication of scaffolds, and the effect of protein addition on the final scaffolds' properties is studied. Collagen and gelatin were the proteins selected and two different concentrations were analyzed (2 and 4 wt/vol%). Different PCL/protein systems were prepared, and a structural, mechanical and functional characterization was performed. The influence of fiber alignment on the properties of the final scaffolds was assessed through morphological, mechanical and biological evaluations. A bioreactor was used to promote cell proliferation and differentiation within the scaffolds. The results revealed that protein addition produced a decrease in the fiber size of the membranes, an increase in their hydrophilicity, and a softening of their mechanical properties. The biological study showed the ability of the selected systems to harbor cells, allow their growth and, potentially, develop musculoskeletal tissues.
AB - The creation of skeletal muscle tissue in vitro is a major topic of interest today in the field of biomedical research, due to the lack of treatments for muscle loss due to traumatic accidents or disease. For this reason, the intrinsic properties of nanofibrillar structures to promote cell adhesion, proliferation, and cell alignment presents an attractive tool for regenerative medicine to recreate organized tissues such as muscle. Electrospinning is one of the processing techniques often used for the fabrication of these nanofibrous structures and the combination of synthetic and natural polymers is often required to achieve optimal mechanical and physiochemical properties. Here, polycaprolactone (PCL) is selected as a synthetic polymer used for the fabrication of scaffolds, and the effect of protein addition on the final scaffolds' properties is studied. Collagen and gelatin were the proteins selected and two different concentrations were analyzed (2 and 4 wt/vol%). Different PCL/protein systems were prepared, and a structural, mechanical and functional characterization was performed. The influence of fiber alignment on the properties of the final scaffolds was assessed through morphological, mechanical and biological evaluations. A bioreactor was used to promote cell proliferation and differentiation within the scaffolds. The results revealed that protein addition produced a decrease in the fiber size of the membranes, an increase in their hydrophilicity, and a softening of their mechanical properties. The biological study showed the ability of the selected systems to harbor cells, allow their growth and, potentially, develop musculoskeletal tissues.
KW - collagen
KW - electrospinning
KW - PCL
KW - scaffolds
KW - skeletal muscle cells
U2 - 10.1002/jbm.a.37156
DO - 10.1002/jbm.a.37156
M3 - Article
C2 - 33665968
SN - 1549-3296
VL - 109
SP - 1600
EP - 1612
JO - Journal of Biomedical Materials Research Part A
JF - Journal of Biomedical Materials Research Part A
IS - 9
ER -