Extended-spectrum beta-lactamase producing Enterobacteriaceae (ESBL-E) isolated from bean sprouts in the Netherlands

Pepijn Huizinga; Eefje Schrauwen; Silvia Garcia-Cobos; Ina Willemsen; Carlo Verhulst; Alexander W. Friedrich; Paul H. M. Savelkoul; John W. Rossen; Jan Kluytmans

doi:10.1371/journal.pone.0203338

Extended-spectrum beta-lactamase producing Enterobacteriaceae (ESBL-E) isolated from bean sprouts in the Netherlands

Pepijn Huizinga^*, Eefje Schrauwen, Silvia Garcia-Cobos, Ina Willemsen, Carlo Verhulst, Alexander W. Friedrich, Paul H. M. Savelkoul, John W. Rossen, Jan Kluytmans

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Community-acquired carriage and infections due to extended-spectrum beta-lactamase producing Enterobacteriaceae (ESBL-E) are increasing worldwide, resulting in increased morbidity, mortality and healthcare costs. The origins of community-acquired ESBL-E-carriage and infections remain unclear. Bean sprouts are a potential source of Enterobacteriaceae for the community, as illustrated by outbreaks of pathogenic Enterobacteriaceae in the past. The current study focuses on contamination of retail bean sprouts with ESBL-E in the Netherlands. Of 131 bean sprout samples purchased between 2013 and 2016, 25 (19%) were contaminated with ESBL-E. The detected isolates were almost exclusively Klebsiella spp. and co-resistance to other antibiotics was observed frequently. Over time there was substantial genetic diversity between isolates. On the other hand, isolates from samples closely matched in time were frequently clonally related, indicative of batch contamination. Remarkably, no Escherichia colt was found. In conclusion, bean sprouts frequently harbor ESBL-E, which is a potential source for consumers.

Original language	English
Article number	e0203338
Number of pages	13
Journal	PLOS ONE
Volume	13
Issue number	8
DOIs	https://doi.org/10.1371/journal.pone.0203338
Publication status	Published - 30 Aug 2018

Keywords

AMPC-PRODUCING ENTEROBACTERIACEAE
ESCHERICHIA-COLI
RISK-FACTORS
COMMUNITY PATIENTS
RESISTANCE GENES
DRINKING-WATER
CHICKEN MEAT
CTX-M
PREVALENCE
CARRIAGE

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https://doi.org/10.1371/journal.pone.0203338

Cite this

@article{4a8136f089b64e919685cddd1208443c,

title = "Extended-spectrum beta-lactamase producing Enterobacteriaceae (ESBL-E) isolated from bean sprouts in the Netherlands",

abstract = "Community-acquired carriage and infections due to extended-spectrum beta-lactamase producing Enterobacteriaceae (ESBL-E) are increasing worldwide, resulting in increased morbidity, mortality and healthcare costs. The origins of community-acquired ESBL-E-carriage and infections remain unclear. Bean sprouts are a potential source of Enterobacteriaceae for the community, as illustrated by outbreaks of pathogenic Enterobacteriaceae in the past. The current study focuses on contamination of retail bean sprouts with ESBL-E in the Netherlands. Of 131 bean sprout samples purchased between 2013 and 2016, 25 (19%) were contaminated with ESBL-E. The detected isolates were almost exclusively Klebsiella spp. and co-resistance to other antibiotics was observed frequently. Over time there was substantial genetic diversity between isolates. On the other hand, isolates from samples closely matched in time were frequently clonally related, indicative of batch contamination. Remarkably, no Escherichia colt was found. In conclusion, bean sprouts frequently harbor ESBL-E, which is a potential source for consumers.",

keywords = "AMPC-PRODUCING ENTEROBACTERIACEAE, ESCHERICHIA-COLI, RISK-FACTORS, COMMUNITY PATIENTS, RESISTANCE GENES, DRINKING-WATER, CHICKEN MEAT, CTX-M, PREVALENCE, CARRIAGE",

author = "Pepijn Huizinga and Eefje Schrauwen and Silvia Garcia-Cobos and Ina Willemsen and Carlo Verhulst and Friedrich, {Alexander W.} and Savelkoul, {Paul H. M.} and Rossen, {John W.} and Jan Kluytmans",

year = "2018",

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doi = "10.1371/journal.pone.0203338",

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issn = "1932-6203",

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AU - Huizinga, Pepijn

AU - Schrauwen, Eefje

AU - Garcia-Cobos, Silvia

AU - Willemsen, Ina

AU - Verhulst, Carlo

AU - Friedrich, Alexander W.

AU - Savelkoul, Paul H. M.

AU - Rossen, John W.

AU - Kluytmans, Jan

PY - 2018/8/30

Y1 - 2018/8/30

N2 - Community-acquired carriage and infections due to extended-spectrum beta-lactamase producing Enterobacteriaceae (ESBL-E) are increasing worldwide, resulting in increased morbidity, mortality and healthcare costs. The origins of community-acquired ESBL-E-carriage and infections remain unclear. Bean sprouts are a potential source of Enterobacteriaceae for the community, as illustrated by outbreaks of pathogenic Enterobacteriaceae in the past. The current study focuses on contamination of retail bean sprouts with ESBL-E in the Netherlands. Of 131 bean sprout samples purchased between 2013 and 2016, 25 (19%) were contaminated with ESBL-E. The detected isolates were almost exclusively Klebsiella spp. and co-resistance to other antibiotics was observed frequently. Over time there was substantial genetic diversity between isolates. On the other hand, isolates from samples closely matched in time were frequently clonally related, indicative of batch contamination. Remarkably, no Escherichia colt was found. In conclusion, bean sprouts frequently harbor ESBL-E, which is a potential source for consumers.

AB - Community-acquired carriage and infections due to extended-spectrum beta-lactamase producing Enterobacteriaceae (ESBL-E) are increasing worldwide, resulting in increased morbidity, mortality and healthcare costs. The origins of community-acquired ESBL-E-carriage and infections remain unclear. Bean sprouts are a potential source of Enterobacteriaceae for the community, as illustrated by outbreaks of pathogenic Enterobacteriaceae in the past. The current study focuses on contamination of retail bean sprouts with ESBL-E in the Netherlands. Of 131 bean sprout samples purchased between 2013 and 2016, 25 (19%) were contaminated with ESBL-E. The detected isolates were almost exclusively Klebsiella spp. and co-resistance to other antibiotics was observed frequently. Over time there was substantial genetic diversity between isolates. On the other hand, isolates from samples closely matched in time were frequently clonally related, indicative of batch contamination. Remarkably, no Escherichia colt was found. In conclusion, bean sprouts frequently harbor ESBL-E, which is a potential source for consumers.

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KW - DRINKING-WATER

KW - CHICKEN MEAT

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KW - PREVALENCE

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