Expression of retinoid X receptor beta is induced in astrocytes during corpus callosum demyelination

Rene Koenig, Milena Stifried, Philipp Aperdannier, Tim Clarner, Cordian Beyer, Markus Kipp, Joerg Mey*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Web of Science)

Abstract

The experimental activation of retinoid receptors reduces pathological symptoms in animal models of multiple sclerosis. In order to assess the involvement of endogenous retinoid signaling during the process of demyelination we investigated retinoic acid synthesizing enzymes and nuclear receptors using the mouse model of cuprizone toxicity. The initiation of myelin degradation in the corpus callosum was accompanied with a local increase of retinaldehyde dehydrogenase (RALDH) immunoreactivity. On the level of receptors we observed a striking increase in protein expression of the retinoid X receptor (RXR)-? in the affected corpus callosum. The RXR? immunoreactivity appeared exclusively in astrocytes, where it reached a maximum at five weeks of treatment, following the RALDH response. In the cerebral cortex and basal ganglia of affected mice RXR? was also observed in neurons. Among nuclear receptor antigens RAR? showed a cuprizone associated increase in the corpus callosum. Quantitative RT-PCR revealed strong basal expression of RXR? and a significant, over 20-fold upregulation of the peroxisome proliferator-activated receptor-? during demyelination. The results indicate that compensatory mechanisms during central demyelination may engage nuclear receptor dimers with an RXR? partner.
Original languageEnglish
Pages (from-to)120-132
JournalJournal of Chemical Neuroanatomy
Volume43
Issue number2
DOIs
Publication statusPublished - Mar 2012

Keywords

  • Cuprizone
  • Retinoic acid
  • Nuclear receptor
  • Astrocyte
  • Myelin
  • Oligodendrocyte

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