TY - JOUR
T1 - Expression of perilipins in human skeletal muscle in vitro and in vivo in relation to diet, exercise and energy balance.
AU - Gjelstad, I.M.
AU - Haugen, F.
AU - Gulseth, H.L.
AU - Norheim, F.
AU - Jans, A.
AU - Bakke, S.S.
AU - Raastad, T.
AU - Tjonna, A.E.
AU - Wisloff, U.
AU - Blaak, E.E.
AU - Riserus, U.
AU - Gaster, M.
AU - Roche, H.M.
AU - Birkeland, K.I.
AU - Drevon, C.A.
PY - 2012/1/1
Y1 - 2012/1/1
N2 - The perilipin proteins enclose intracellular lipid droplets. We describe the mRNA expression of the five perilipins in human skeletal muscle in relation to fatty acid supply, exercise and energy balance. We observed that all perilipins were expressed in skeletal muscle biopsies with the highest mRNA levels of perilipin 2, 4 and 5. Cultured myotubes predominantly expressed perilipin 2 and 3. In vitro, incubation of myotubes with fatty acids enhanced mRNA expression of perilipin 1, 2 and 4. In vivo, low fat diet increased mRNA levels of perilipin 3 and 4. Endurance training, but not strength training, enhanced the expression of perilipin 2 and 3. Perilipin 1 mRNA correlated positively with body fat mass, whereas none of the perilipins were associated with insulin sensitivity. In conclusion, all perilipins mRNAs were expressed in human skeletal muscle. Diet as well as endurance exercise modulated the expression of perilipins.
AB - The perilipin proteins enclose intracellular lipid droplets. We describe the mRNA expression of the five perilipins in human skeletal muscle in relation to fatty acid supply, exercise and energy balance. We observed that all perilipins were expressed in skeletal muscle biopsies with the highest mRNA levels of perilipin 2, 4 and 5. Cultured myotubes predominantly expressed perilipin 2 and 3. In vitro, incubation of myotubes with fatty acids enhanced mRNA expression of perilipin 1, 2 and 4. In vivo, low fat diet increased mRNA levels of perilipin 3 and 4. Endurance training, but not strength training, enhanced the expression of perilipin 2 and 3. Perilipin 1 mRNA correlated positively with body fat mass, whereas none of the perilipins were associated with insulin sensitivity. In conclusion, all perilipins mRNAs were expressed in human skeletal muscle. Diet as well as endurance exercise modulated the expression of perilipins.
U2 - 10.3109/13813455.2011.630009
DO - 10.3109/13813455.2011.630009
M3 - Article
C2 - 22117101
SN - 1381-3455
VL - 118
SP - 22
EP - 30
JO - Archives of Physiology and Biochemistry
JF - Archives of Physiology and Biochemistry
IS - 1
ER -