Exploration of the Esophageal Mucosal Barrier in Non-Erosive Reflux Disease

Nicolaas F. Rinsma, Ricard Farre, Fred J. Troost, Montserrat Elizalde, Daniel Keszthelyi, Zsuzsanna Helyes, Ad A. Masclee, Jose M. Conchillo*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Web of Science)

Abstract

In the absence of visible mucosal damage, it is hypothesized that the esophageal mucosal barrier is functionally impaired in patients with non-erosive reflux disease (NERD). The aim of the present study was to perform an exploratory analysis of the mucosal barrier in NERD compared to erosive esophagitis (EE) and controls. A second aim was to explore TRPV1 gene transcription in relation to the mucosal barrier function and heartburn symptoms. In this prospective study, 10 NERD patients, 11 patients with active erosive esophagitis and 10 healthy volunteers were included. Biopsies from non-eroded mucosa were obtained for (1) ex vivo analyses (Ussing chamber) of transepithelial electrical resistance (TEER) and permeability (2) gene transcription of tight-junction proteins and transient receptor potential vanilloid subfamily member 1 (TRPV1). No differences in TEER or permeability were found between NERD and healthy volunteers, whereas TEER was lower in patients with erosive esophagitis. TRPV1 gene transcription was not significantly different between EE, NERD and controls. Conclusions: esophageal mucosal barrier function and TRPV1 transcription is not significantly altered in NERD patients. Future research is needed to explore other potential mechanisms that may account for the high symptom burden in these patients.

Original languageEnglish
Article number1091
Number of pages12
JournalInternational journal of molecular sciences
Volume18
Issue number5
DOIs
Publication statusPublished - May 2017

Keywords

  • mucosal integrity
  • Ussing chamber
  • erosive esophagitis
  • non-erosive reflux disease
  • transepithelial electrical resistance
  • TRPV1
  • heartburn
  • DILATED INTERCELLULAR SPACES
  • BASE-LINE IMPEDANCE
  • CAPSAICIN RECEPTOR TRPV1
  • IRRITABLE-BOWEL-SYNDROME
  • GASTROESOPHAGEAL-REFLUX
  • VISCERAL HYPERSENSITIVITY
  • FUNCTIONAL HEARTBURN
  • ACID EXPOSURE
  • GERD PATIENTS
  • NERVE-FIBERS

Cite this