Exploitation of the ribosomal protein L10 R98S mutation to enhance recombinant protein production in mammalian cells

B. Verbelen; T. Girardi; S.O. Sulima; S. Vereecke; P. Verstraete; J. Verbeeck; J. Royaert; S. Cinque; L. Montanaro; M. Penzo; M. Imbrechts; N. Geukens; T. Geuens; K. Dierckx; D. Pepe; K. Kampen; K. De Keersmaecker

doi:10.1002/elsc.202100124

Exploitation of the ribosomal protein L10 R98S mutation to enhance recombinant protein production in mammalian cells

B. Verbelen, T. Girardi, S.O. Sulima, S. Vereecke, P. Verstraete, J. Verbeeck, J. Royaert, S. Cinque, L. Montanaro, M. Penzo, M. Imbrechts, N. Geukens, T. Geuens, K. Dierckx, D. Pepe, K. Kampen, K. De Keersmaecker^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Original language	English
Pages (from-to)	100-114
Number of pages	15
Journal	Engineering in Life Sciences
Volume	22
Issue number	2
Early online date	14 Jan 2022
DOIs	https://doi.org/10.1002/elsc.202100124
Publication status	Published - Feb 2022

Keywords

genome engineering
recombinant protein production
ribosomal protein mutation
RPL10
GENE KNOCKOUT
CHO-CELLS
NUCLEASE

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10.1002/elsc.202100124Licence: CC BY

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Verbelen, B., Girardi, T., Sulima, S. O., Vereecke, S., Verstraete, P., Verbeeck, J., Royaert, J., Cinque, S., Montanaro, L., Penzo, M., Imbrechts, M., Geukens, N., Geuens, T., Dierckx, K., Pepe, D., Kampen, K., & De Keersmaecker, K. (2022). Exploitation of the ribosomal protein L10 R98S mutation to enhance recombinant protein production in mammalian cells. Engineering in Life Sciences, 22(2), 100-114. https://doi.org/10.1002/elsc.202100124

Verbelen, B. ; Girardi, T. ; Sulima, S.O. ; Vereecke, S. ; Verstraete, P. ; Verbeeck, J. ; Royaert, J. ; Cinque, S. ; Montanaro, L. ; Penzo, M. ; Imbrechts, M. ; Geukens, N. ; Geuens, T. ; Dierckx, K. ; Pepe, D. ; Kampen, K. ; De Keersmaecker, K. / Exploitation of the ribosomal protein L10 R98S mutation to enhance recombinant protein production in mammalian cells. In: Engineering in Life Sciences. 2022 ; Vol. 22, No. 2. pp. 100-114.

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title = "Exploitation of the ribosomal protein L10 R98S mutation to enhance recombinant protein production in mammalian cells",

keywords = "genome engineering, recombinant protein production, ribosomal protein mutation, RPL10, GENE KNOCKOUT, CHO-CELLS, NUCLEASE",

author = "B. Verbelen and T. Girardi and S.O. Sulima and S. Vereecke and P. Verstraete and J. Verbeeck and J. Royaert and S. Cinque and L. Montanaro and M. Penzo and M. Imbrechts and N. Geukens and T. Geuens and K. Dierckx and D. Pepe and K. Kampen and {De Keersmaecker}, K.",

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doi = "10.1002/elsc.202100124",

language = "English",

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Verbelen, B, Girardi, T, Sulima, SO, Vereecke, S, Verstraete, P, Verbeeck, J, Royaert, J, Cinque, S, Montanaro, L, Penzo, M, Imbrechts, M, Geukens, N, Geuens, T, Dierckx, K, Pepe, D, Kampen, K & De Keersmaecker, K 2022, 'Exploitation of the ribosomal protein L10 R98S mutation to enhance recombinant protein production in mammalian cells', Engineering in Life Sciences, vol. 22, no. 2, pp. 100-114. https://doi.org/10.1002/elsc.202100124

Exploitation of the ribosomal protein L10 R98S mutation to enhance recombinant protein production in mammalian cells. / Verbelen, B.; Girardi, T.; Sulima, S.O.; Vereecke, S.; Verstraete, P.; Verbeeck, J.; Royaert, J.; Cinque, S.; Montanaro, L.; Penzo, M.; Imbrechts, M.; Geukens, N.; Geuens, T.; Dierckx, K.; Pepe, D.; Kampen, K.; De Keersmaecker, K.

In: Engineering in Life Sciences, Vol. 22, No. 2, 02.2022, p. 100-114.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Exploitation of the ribosomal protein L10 R98S mutation to enhance recombinant protein production in mammalian cells

AU - Verbelen, B.

AU - Girardi, T.

AU - Sulima, S.O.

AU - Vereecke, S.

AU - Verstraete, P.

AU - Verbeeck, J.

AU - Royaert, J.

AU - Cinque, S.

AU - Montanaro, L.

AU - Penzo, M.

AU - Imbrechts, M.

AU - Geukens, N.

AU - Geuens, T.

AU - Dierckx, K.

AU - Pepe, D.

AU - Kampen, K.

AU - De Keersmaecker, K.

PY - 2022/2

Y1 - 2022/2

KW - genome engineering

KW - recombinant protein production

KW - ribosomal protein mutation

KW - RPL10

KW - GENE KNOCKOUT

KW - CHO-CELLS

KW - NUCLEASE

U2 - 10.1002/elsc.202100124

DO - 10.1002/elsc.202100124

M3 - Article

C2 - 35140557

VL - 22

SP - 100

EP - 114

JO - Engineering in Life Sciences

JF - Engineering in Life Sciences

SN - 1618-0240

IS - 2

ER -