Exosomal microRNA miR-92a concentration in serum reflects human brown fat activity

Yong Chen; Joschka J. Buyel; Mark J. W. Hanssen; Franziska Siegel; Ruping Pan; Jennifer Naumann; Michael Schell; Anouk van der Lans; Christian Schlein; Holger Froehlich; Joerg Heeren; Kirsi A. Virtanen; Wouter van Marken Lichtenbelt; Alexander Pfeifer

doi:10.1038/ncomms11420

Exosomal microRNA miR-92a concentration in serum reflects human brown fat activity

Yong Chen, Joschka J. Buyel, Mark J. W. Hanssen, Franziska Siegel, Ruping Pan, Jennifer Naumann, Michael Schell, Anouk van der Lans, Christian Schlein, Holger Froehlich, Joerg Heeren, Kirsi A. Virtanen, Wouter van Marken Lichtenbelt, Alexander Pfeifer^*

^*Corresponding author for this work

Humane Biologie

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Brown adipose tissue (BAT) dissipates energy and its activity correlates with leanness in human adults. F-18-fluorodeoxyglucose (F-18-FDG) positron emission tomography coupled with computer tomography (PET/CT) is still the standard for measuring BAT activity, but exposes subjects to ionizing radiation. To study BAT function in large human cohorts, novel diagnostic tools are needed. Here we show that brown adipocytes release exosomes and that BAT activation increases exosome release. Profiling miRNAs in exosomes released from brown adipocytes, and in exosomes isolated from mouse serum, we show that levels of miRNAs change after BAT activation in vitro and in vivo. One of these exosomal miRNAs, miR-92a, is also present in human serum exosomes. Importantly, serum concentrations of exosomal miR-92a inversely correlate with human BAT activity measured by F-18-FDG PET/CT in two unique and independent cohorts comprising 41 healthy individuals. Thus, exosomal miR-92a represents a potential serum biomarker for BAT activity in mice and humans.

Original language	English
Article number	11420
Journal	Nature Communications
Volume	7
DOIs	https://doi.org/10.1038/ncomms11420
Publication status	Published - Apr 2016

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Chen, Y., Buyel, J. J., Hanssen, M. J. W., Siegel, F., Pan, R., Naumann, J., Schell, M., van der Lans, A., Schlein, C., Froehlich, H., Heeren, J., Virtanen, K. A., van Marken Lichtenbelt, W., & Pfeifer, A. (2016). Exosomal microRNA miR-92a concentration in serum reflects human brown fat activity. Nature Communications, 7, Article 11420. https://doi.org/10.1038/ncomms11420

@article{d7cd130430e34a5bae8493d7f9950446,

title = "Exosomal microRNA miR-92a concentration in serum reflects human brown fat activity",

abstract = "Brown adipose tissue (BAT) dissipates energy and its activity correlates with leanness in human adults. F-18-fluorodeoxyglucose (F-18-FDG) positron emission tomography coupled with computer tomography (PET/CT) is still the standard for measuring BAT activity, but exposes subjects to ionizing radiation. To study BAT function in large human cohorts, novel diagnostic tools are needed. Here we show that brown adipocytes release exosomes and that BAT activation increases exosome release. Profiling miRNAs in exosomes released from brown adipocytes, and in exosomes isolated from mouse serum, we show that levels of miRNAs change after BAT activation in vitro and in vivo. One of these exosomal miRNAs, miR-92a, is also present in human serum exosomes. Importantly, serum concentrations of exosomal miR-92a inversely correlate with human BAT activity measured by F-18-FDG PET/CT in two unique and independent cohorts comprising 41 healthy individuals. Thus, exosomal miR-92a represents a potential serum biomarker for BAT activity in mice and humans.",

author = "Yong Chen and Buyel, {Joschka J.} and Hanssen, {Mark J. W.} and Franziska Siegel and Ruping Pan and Jennifer Naumann and Michael Schell and {van der Lans}, Anouk and Christian Schlein and Holger Froehlich and Joerg Heeren and Virtanen, {Kirsi A.} and {van Marken Lichtenbelt}, Wouter and Alexander Pfeifer",

year = "2016",

month = apr,

doi = "10.1038/ncomms11420",

language = "English",

volume = "7",

journal = "Nature Communications",

issn = "2041-1723",

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T1 - Exosomal microRNA miR-92a concentration in serum reflects human brown fat activity

AU - Chen, Yong

AU - Buyel, Joschka J.

AU - Hanssen, Mark J. W.

AU - Siegel, Franziska

AU - Pan, Ruping

AU - Naumann, Jennifer

AU - Schell, Michael

AU - van der Lans, Anouk

AU - Schlein, Christian

AU - Froehlich, Holger

AU - Heeren, Joerg

AU - Virtanen, Kirsi A.

AU - van Marken Lichtenbelt, Wouter

AU - Pfeifer, Alexander

PY - 2016/4

Y1 - 2016/4

N2 - Brown adipose tissue (BAT) dissipates energy and its activity correlates with leanness in human adults. F-18-fluorodeoxyglucose (F-18-FDG) positron emission tomography coupled with computer tomography (PET/CT) is still the standard for measuring BAT activity, but exposes subjects to ionizing radiation. To study BAT function in large human cohorts, novel diagnostic tools are needed. Here we show that brown adipocytes release exosomes and that BAT activation increases exosome release. Profiling miRNAs in exosomes released from brown adipocytes, and in exosomes isolated from mouse serum, we show that levels of miRNAs change after BAT activation in vitro and in vivo. One of these exosomal miRNAs, miR-92a, is also present in human serum exosomes. Importantly, serum concentrations of exosomal miR-92a inversely correlate with human BAT activity measured by F-18-FDG PET/CT in two unique and independent cohorts comprising 41 healthy individuals. Thus, exosomal miR-92a represents a potential serum biomarker for BAT activity in mice and humans.

AB - Brown adipose tissue (BAT) dissipates energy and its activity correlates with leanness in human adults. F-18-fluorodeoxyglucose (F-18-FDG) positron emission tomography coupled with computer tomography (PET/CT) is still the standard for measuring BAT activity, but exposes subjects to ionizing radiation. To study BAT function in large human cohorts, novel diagnostic tools are needed. Here we show that brown adipocytes release exosomes and that BAT activation increases exosome release. Profiling miRNAs in exosomes released from brown adipocytes, and in exosomes isolated from mouse serum, we show that levels of miRNAs change after BAT activation in vitro and in vivo. One of these exosomal miRNAs, miR-92a, is also present in human serum exosomes. Importantly, serum concentrations of exosomal miR-92a inversely correlate with human BAT activity measured by F-18-FDG PET/CT in two unique and independent cohorts comprising 41 healthy individuals. Thus, exosomal miR-92a represents a potential serum biomarker for BAT activity in mice and humans.

U2 - 10.1038/ncomms11420

DO - 10.1038/ncomms11420

M3 - Article

C2 - 27117818

SN - 2041-1723

VL - 7

JO - Nature Communications

JF - Nature Communications

M1 - 11420

ER -

Exosomal microRNA miR-92a concentration in serum reflects human brown fat activity

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