Ex Vivo Culture Models to Indicate Therapy Response in Head and Neck Squamous Cell Carcinoma

Imke Demers, Johan Donkers, Bernd Kremer, Ernst Jan Speel*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

6 Citations (Web of Science)

Abstract

Head and neck squamous cell carcinoma (HNSCC) is characterized by a poor 5 year survival and varying response rates to both standard-of-care and new treatments. Despite advances in medicine and treatment methods, mortality rates have hardly decreased in recent decades. Reliable patient-derived tumor models offer the chance to predict therapy response in a personalized setting, thereby improving treatment efficacy by identifying the most appropriate treatment regimen for each patient. Furthermore, ex vivo tumor models enable testing of novel therapies before introduction in clinical practice. A literature search was performed to identify relevant literature describing three-dimensional ex vivo culture models of HNSCC to examine sensitivity to chemotherapy, radiotherapy, immunotherapy and targeted therapy. We provide a comprehensive overview of the currently used three-dimensional ex vivo culture models for HNSCC with their advantages and limitations, including culture success percentage and comparison to the original tumor. Furthermore, we evaluate the potential of these models to predict patient therapy response.

Original languageEnglish
Article number2527
Number of pages27
JournalCells
Volume9
Issue number11
DOIs
Publication statusPublished - Nov 2020

Keywords

  • head and neck cancer
  • primary cell culture
  • 3D cell culture
  • personalized therapy
  • preclinical prediction model
  • sensitivity testing
  • ex vivo model
  • drug response
  • organoid
  • histoculture
  • EPIDERMAL-GROWTH-FACTOR
  • IN-VITRO CHEMOSENSITIVITY
  • CANCER STEM-CELLS
  • DRUG-RESPONSE
  • AUTOLOGOUS MONOCYTES
  • CLINICAL-RESPONSE
  • SECRETE IL-6
  • TUMOR
  • CHEMOTHERAPY
  • ACTIVATION

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