Evaluation of the Immunoquick+4 malaria rapid diagnostic test in a non-endemic setting

D. P. J. van Dijk, P. Gillet*, E. Vlieghe, Lieselotte Cnops, Marjan van Esbroeck, J. Jacobs

*Corresponding author for this work

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The aim of this retrospective study was to evaluate the Immunoquick+4 (BioSynex, Strasbourg, France), a three-band malaria rapid diagnostic test (MRDT) targeting histidine-rich protein-2 (HRP-2) and pan Plasmodium-specific parasite lactate dehydrogenase, in a non-endemic reference setting. Stored whole-blood samples (n = 613) from international travellers suspected of malaria were used, with microscopy corrected by polymerase chain reaction (PCR) as the reference method. Samples infected by P. falciparum (n = 323), P. vivax (n = 97), P. ovale (n = 73) and P. malariae (n = 25) were selected, as well as 95 malaria-negative samples. The overall sensitivities of the Immunoquick+4 for the diagnosis of P. falciparum, P. vivax, P. malariae and P. ovale were 88.9, 75.3, 56.0 and 19.2%, respectively. Sensitivity was significantly related to parasite density for P. falciparum (93.6% versus 71.4% at parasite densities > 100/A mu l and a parts per thousand currency sign100/A mu l, respectively) and P. vivax (86.8% versus 48.3% at parasite densities > 500/A mu l and a parts per thousand currency sign500/A mu l, respectively). The Immunoquick+4 showed good reproducibility and reliability for both test results and line intensities. The Immunoquick+4 performed well for the detection of P. falciparum and P. vivax.
Original languageEnglish
Pages (from-to)577-583
JournalEuropean Journal of Clinical Microbiology & Infectious Diseases
Issue number5
Publication statusPublished - May 2010

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