Evaluation of Delta(9)-tetrahydrocannabinol detection using DrugWipe5S((R)) screening and oral fluid quantification after Quantisal (TM) collection for roadside drug detection via a controlled study with chronic cannabis users

Oral fluid (OF) is potentially useful to detect driving under the influence of drugs because of its ease of sampling. While cannabis is the most prevalent drug in Europe, sensitivity issues for (9)-tetrahydrocannabinol (THC) screening and problems during OF collection are observed. The ability of a recently improved OF screening device - the DrugWipe5S((R)), to detect recent THC use in chronic cannabis smokers, was studied. Ten subjects participated in a double-blind placebo-controlled study. The subjects smoked two subsequent doses of THC; 300 mu g/kg and 150 mu g/kg with a pause of 75min using a Volcano vapourizer. DrugWipe5S((R)) screening and OF collection using the Quantisal device were performed at baseline, 5min after each administration and 80min after the last inhalation. Blood samples were drawn simultaneously. The screening devices (n=80) were evaluated visually after 8min, while the corresponding OF and serum samples were analyzed respectively with ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) or gas chromatography-mass spectrometry (GC-MS). Neat OF THC concentrations ranged from 12 361ng/g 5min after smoking down to 34ng/g 80min later. Under placebo conditions, a median THC concentration of 8ng/g OF (0-746ng/g) and