TY - JOUR
T1 - Evaluation of Anti-PLA2R1 as Measured by a Novel ELISA in Patients With Idiopathic Membranous Nephropathy A Cohort Study
AU - Timmermans, Sjoerd A. M. E. G.
AU - Damoiseaux, Jan G. M. C.
AU - Heerings-Rewinkel, Petra T. J.
AU - Ayalon, Rivka
AU - Beck, Laurence H., Jr.
AU - Schlumberger, Wolfgang
AU - Salant, David J.
AU - van Paassen, Pieter
AU - Tervaert, Jan Willem Cohen
PY - 2014/7
Y1 - 2014/7
N2 - Objectives: Autoantibodies against the M-type phospholipase A2 receptor 1 (anti-PLA2R1) have been demonstrated to be very specific for idiopathic membranous nephropathy (MN). We studied a novel enzyme-linked immunosorbent assay (ELISA) and compared results with results obtained using an indirect immunofluorescence (IF) and a Western blotting test (WB). Methods: One-hundred nine patients with idiopathic MN were recruited between November 1979 and March 2011. The control cohort comprised serum samples from patients with secondary MN (n = 16) and nephrotic controls (n = 17). The presence of anti-PLA2R1 in serum samples obtained at the time of renal biopsy was determined using ELISA, IIF, and WB. Results: With similar specificity (>= 97%), sensitivity varied from 68% (IIF) to 72% (ELISA, WB). Remarkably, patients who were seronegative for anti-PLA2R1 more often entered spontaneous remission (P = .038), whereas seropositive patients were more frequently treated with immunosuppressive agents (P <.001). Conclusions: ELISA performs excellently in differentiating idiopathic from secondary MN. Furthermore, ELISA shared high agreement with WB and IIF.
AB - Objectives: Autoantibodies against the M-type phospholipase A2 receptor 1 (anti-PLA2R1) have been demonstrated to be very specific for idiopathic membranous nephropathy (MN). We studied a novel enzyme-linked immunosorbent assay (ELISA) and compared results with results obtained using an indirect immunofluorescence (IF) and a Western blotting test (WB). Methods: One-hundred nine patients with idiopathic MN were recruited between November 1979 and March 2011. The control cohort comprised serum samples from patients with secondary MN (n = 16) and nephrotic controls (n = 17). The presence of anti-PLA2R1 in serum samples obtained at the time of renal biopsy was determined using ELISA, IIF, and WB. Results: With similar specificity (>= 97%), sensitivity varied from 68% (IIF) to 72% (ELISA, WB). Remarkably, patients who were seronegative for anti-PLA2R1 more often entered spontaneous remission (P = .038), whereas seropositive patients were more frequently treated with immunosuppressive agents (P <.001). Conclusions: ELISA performs excellently in differentiating idiopathic from secondary MN. Furthermore, ELISA shared high agreement with WB and IIF.
KW - Membranous nephropathy
KW - Phospholipase A2 receptor 1
KW - Nephrotic syndrome
KW - ELISA
KW - Sensitivity and specificity
U2 - 10.1309/AJCP8QMOY5GLRSFP
DO - 10.1309/AJCP8QMOY5GLRSFP
M3 - Article
C2 - 24926082
SN - 0002-9173
VL - 142
SP - 29
EP - 34
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
IS - 1
ER -